T23 2010 1.indd
Increase of intracellular calcium concentration and tyrosine kinase involvement are pivotal in sperm capacitation. The aim was to determine the involvement of intracellular calcium and tyrosine kinases activity in frozen-thawed spermatozoa capacitated with heparin or quercetin. Genistein or PP2 (4-a...
| Autores principales: | , |
|---|---|
| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2010
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| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4990 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4990.dir/4990.PDF |
| Aporte de: |
| id |
I28-R145-HWA_4990 |
|---|---|
| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
calcio capacitación bovina SRC tirosina quinasa calcium bovine capacitation SRC tyrosine kinases |
| spellingShingle |
calcio capacitación bovina SRC tirosina quinasa calcium bovine capacitation SRC tyrosine kinases Satorre, M. Córdoba, M. T23 2010 1.indd |
| topic_facet |
calcio capacitación bovina SRC tirosina quinasa calcium bovine capacitation SRC tyrosine kinases |
| description |
Increase of intracellular calcium concentration and tyrosine kinase involvement are pivotal in sperm capacitation. The aim was to determine the involvement of intracellular calcium and tyrosine kinases activity in frozen-thawed spermatozoa capacitated with heparin or quercetin. Genistein or PP2 (4-amino 5-(4-chlorophenyl)-7-(t-butyl)pyrazolo3,4, pyrimidine) were used to inhibit tyrosine kinases, and methoxyverapamil to inhibit voltage dependent calcium channels. Capacitation was determined by chlortetracycline and calcium by fluorescence spectrophotometry. Protein tyrosine-phosphorylation was determined by western blot. Pooled frozen semen samples from four bulls were used. In presence of heparin or quercetin capacitated spermatozoa percentage and intracellular calcium were greater (P<0.05) than in controls. Genistein, or PP2 addition in heparin treated samples decreased (P<0.05) capacitation and calcium, but not in quercetin treated samples. A differential inhibition of protein phosphorylation pattern was observed in heparin or quercetin treated suspension in presence of genistein or PP2. Methoxyverapamil blocked calcium increase in heparin, but not in quercetin treated samples. Capacitated spermatozoa percentage and calcium increase were similar with both inductors suggesting that they are capable to provoke capacitation by different mechanisms that dependent or not dependent on voltage dependent calcium channels, thus different intracellular compartments may be involved. In cryopreserved bovine spermatozoa tyrosine kinases including SRC-isoform modulate capacitation. |
| format |
Artículo Artículo publishedVersion |
| author |
Satorre, M. Córdoba, M. |
| author_facet |
Satorre, M. Córdoba, M. |
| author_sort |
Satorre, M. |
| title |
T23 2010 1.indd |
| title_short |
T23 2010 1.indd |
| title_full |
T23 2010 1.indd |
| title_fullStr |
T23 2010 1.indd |
| title_full_unstemmed |
T23 2010 1.indd |
| title_sort |
t23 2010 1.indd |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2010 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4990 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4990.dir/4990.PDF |
| work_keys_str_mv |
AT satorrem t2320101indd AT cordobam t2320101indd AT satorrem involvementofintracellularcalciumandsrctyrosinekinaseincapacitationofcryopreservedbovinespermatozoa AT cordobam involvementofintracellularcalciumandsrctyrosinekinaseincapacitationofcryopreservedbovinespermatozoa AT satorrem participaciondelcalciointracelularysrctirosinaquinasasenlacapacitaciondelespermatozoidebovinocriopreservado AT cordobam participaciondelcalciointracelularysrctirosinaquinasasenlacapacitaciondelespermatozoidebovinocriopreservado |
| _version_ |
1824359772183330816 |
| spelling |
I28-R145-HWA_49902024-11-05 T23 2010 1.indd Increase of intracellular calcium concentration and tyrosine kinase involvement are pivotal in sperm capacitation. The aim was to determine the involvement of intracellular calcium and tyrosine kinases activity in frozen-thawed spermatozoa capacitated with heparin or quercetin. Genistein or PP2 (4-amino 5-(4-chlorophenyl)-7-(t-butyl)pyrazolo3,4, pyrimidine) were used to inhibit tyrosine kinases, and methoxyverapamil to inhibit voltage dependent calcium channels. Capacitation was determined by chlortetracycline and calcium by fluorescence spectrophotometry. Protein tyrosine-phosphorylation was determined by western blot. Pooled frozen semen samples from four bulls were used. In presence of heparin or quercetin capacitated spermatozoa percentage and intracellular calcium were greater (P<0.05) than in controls. Genistein, or PP2 addition in heparin treated samples decreased (P<0.05) capacitation and calcium, but not in quercetin treated samples. A differential inhibition of protein phosphorylation pattern was observed in heparin or quercetin treated suspension in presence of genistein or PP2. Methoxyverapamil blocked calcium increase in heparin, but not in quercetin treated samples. Capacitated spermatozoa percentage and calcium increase were similar with both inductors suggesting that they are capable to provoke capacitation by different mechanisms that dependent or not dependent on voltage dependent calcium channels, thus different intracellular compartments may be involved. In cryopreserved bovine spermatozoa tyrosine kinases including SRC-isoform modulate capacitation. Fil: Satorre, M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Química Biológica. Buenos Aires, Argentina Fil: Córdoba, M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Química Biológica. Buenos Aires, Argentina Satorre, M. Córdoba, M. 2010 El incremento de calcio intracelular [Ca]i y la participación de tirosina quinasa son pasos cruciales en la inducción de la capacitación. El objetivo fue determinar en espermatozoides criopreservados, la variación [Ca]i y la actividad de tirosina quinasa en la capacitación con heparina o quercetina. Genisteina o PP2(4-amino-5-(4-clorofenil)-7-(t-butil)pirazolo3,4,d pirimidina) son inhibidores de tirosinas quinasas y de la isoforma SRC, respectivamente. Metoxiverapamil es un inhibidor de canales de calcio voltaje dependiente (CCVD). La capacitación, [Ca]i y fosforilación en tirosina se evaluaron con clorotetraciclina, espectrofl uorometría y western blot, respectivamente. El porcentaje de espermatozoides capacitados y [Ca]i fueron mayores (P<0.05) en muestras con heparina o quercetina respecto a sus controles. Genisteina o PP2 disminuyeron (P<0.05) la capacitación y el incremento de [Ca]i en las muestras con heparina pero no en las tratadas con quercetina. Genisteina o PP2 inhibió diferencialmente la fosforilación de proteínas espermáticas con ambos inductores. Methoxiverapamil bloqueó el incremento de [Ca]i sólo en la muestras con heparina. Siendo los espermatozoides capacitados y [Ca]i similares con heparina o quercetina, sugiere que la capacitación con ambos inductores es inducida por mecanismos dependientes y no dependientes de CCVD, involucrando así diferentes compartimientos intracelulares. En bovino, las tirosinas quinasas, incluída la isoforma SRC, modulan la inducción de la capacitación. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) calcio capacitación bovina SRC tirosina quinasa calcium bovine capacitation SRC tyrosine kinases eng Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 12, nº1 Involvement of intracellular calcium and src tyrosine-kinase in capacitation of cryopreserved bovine spermatozoa Participación del calcio intracelular y src tirosina quinasas en la capacitación del espermatozoide bovino criopreservado. info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4990 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4990.dir/4990.PDF |