SINTITUL-4

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Leather industry produces potentially hazardous waste which depends of the unhairing process employed by the tannery.To diminish pollution, modified unhairing systems had been developed enzymatic among them. It is important to know the processes enzymes have to go trough, to reach its site of action...

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Autores principales:	Garro M.L., Barbeito C., Mario R.C., Galarza B.C., Cantera C.S.
Formato:	Artículo publishedVersion
Lenguaje:	Español
Publicado:	Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. 2008
Materias:	enzimas depilatorias histología epidermis Histology Depilatory enzymes
Acceso en línea:	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4925 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4925.dir/4925.PDF
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-HWA_4925
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
language	Español
orig_language_str_mv	spa
topic	enzimas depilatorias histología epidermis Histology Depilatory enzymes epidermis
spellingShingle	enzimas depilatorias histología epidermis Histology Depilatory enzymes epidermis Garro M.L. Barbeito C. Mario R.C. Galarza B.C. Cantera C.S. SINTITUL-4
topic_facet	enzimas depilatorias histología epidermis Histology Depilatory enzymes epidermis
description	Leather industry produces potentially hazardous waste which depends of the unhairing process employed by the tannery.To diminish pollution, modified unhairing systems had been developed enzymatic among them. It is important to know the processes enzymes have to go trough, to reach its site of action. In the epidermis at stratum corneum, corneocytes are surrounded by lipids which plays a crucial role as hydrophobic barrier. This barrier must be transformed to allow the enzymes reach its site of action: the follicle-hair and basal membrane. That structures must be eliminated without collagen damage. Collagen is the principal protein related with leather quality. In this study, the effect of different treatments to allow enzyme depilatory passage through epidermis, was analyzed in bovine skin pieces. First, surfactants and sodium sulphite were employed as penetration enhancers followed by and alkaline and pancreatic proteases. Bicarbonate buffer was used as control. Samples were treated with histological techniques; Hematoxilin and Eosin and Masson's Trichrome stain. In the samples under enzymatic treatment, the changes that we have found, could be responsible of leather alteration. Histological study allows to evaluate the changes produced by the process of transforming bovine skin in to leather.\n
format	Artículo Artículo publishedVersion
author	Garro M.L. Barbeito C. Mario R.C. Galarza B.C. Cantera C.S.
author_facet	Garro M.L. Barbeito C. Mario R.C. Galarza B.C. Cantera C.S.
author_sort	Garro M.L.
title	SINTITUL-4
title_short	SINTITUL-4
title_full	SINTITUL-4
title_fullStr	SINTITUL-4
title_full_unstemmed	SINTITUL-4
title_sort	sintitul-4
publisher	Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
publishDate	2008
url	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4925 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4925.dir/4925.PDF
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spelling	I28-R145-HWA_49252024-11-05 SINTITUL-4 Leather industry produces potentially hazardous waste which depends of the unhairing process employed by the tannery.To diminish pollution, modified unhairing systems had been developed enzymatic among them. It is important to know the processes enzymes have to go trough, to reach its site of action. In the epidermis at stratum corneum, corneocytes are surrounded by lipids which plays a crucial role as hydrophobic barrier. This barrier must be transformed to allow the enzymes reach its site of action: the follicle-hair and basal membrane. That structures must be eliminated without collagen damage. Collagen is the principal protein related with leather quality. In this study, the effect of different treatments to allow enzyme depilatory passage through epidermis, was analyzed in bovine skin pieces. First, surfactants and sodium sulphite were employed as penetration enhancers followed by and alkaline and pancreatic proteases. Bicarbonate buffer was used as control. Samples were treated with histological techniques; Hematoxilin and Eosin and Masson's Trichrome stain. In the samples under enzymatic treatment, the changes that we have found, could be responsible of leather alteration. Histological study allows to evaluate the changes produced by the process of transforming bovine skin in to leather.\n Fil: Garro, M.L. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA). Centro de Investigación y Tecnología del Cuero (CITEC). Buenos Aires, Argentina Fil: Barbeito C. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Buenos Aires, Argentina Fil: Barbeito C. CONICET. Argentina Fil: Mario R.C. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Buenos Aires, Argentina Fil: Galarza B.C. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA). Centro de Investigación y Tecnología del Cuero (CITEC). Buenos Aires, Argentina Fil: Cantera C.S. Comisión de Investigaciones Científicas Provincia de Buenos Aires (CICPBA). Buenos Aires, Argentina Garro M.L. Barbeito C. Mario R.C. Galarza B.C. Cantera C.S. 2008 La industria curtidora genera una variedad de desechos potencialmente tóxicos que dependen del proceso de depilado utilizado. Para atenuar esta contaminación, se desarrollaron sistemas alternativos de depilado, entre ellos el enzimático. En la epidermis, a nivel del estrato corneo, se ubican lípidos que ocupan casi por completo el espacio intercelular entre los queratinocitos y desempeñan un importante papel como barrera hidrofóbica. Esta barrera debe transformarse para permitir el paso de las enzimas depilatorias hacia sus sitios de acción: folículo piloso/pelo y membrana basal. Las estructuras mencionadas deben eliminarse sin dañar el colágeno, proteína determinante de la calidad del cuero. Sobre trozos de piel bovina fueron analizados los efectos de diferentes pretratamientos tendientes a favorecer la penetración de enzimas depilatorias. Se utilizaron tensioactivos y sulfito de sodio como pretratamiento y posteriormente tratamientos con proteasas comerciales, una de origen pancréático y otra alcalina. El control fue tratado con buffer de bicarbonato de sodio. Las muestras se colorearon con Hematoxilina Eosina y Tricrómico de Masson. En los cortes sometidos a acción enzimática se encontraron cambios en el colágeno que podrían alterar la calidad del cuero.Se concluye que el estudio histológico de la piel permite evaluar los cambios que pueden ocasionar los tratamientos que se realizan para convertirla en cuero. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) enzimas depilatorias histología epidermis Histology Depilatory enzymes epidermis spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 10, nº2 Evaluación histológica del efecto de enzimas con actividad depilatoria sobre piel vacuna. Un estudio preliminar G Histologically evaluation of the action of depilatory enzimes on bovine skin. A prelimary study. info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4925 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4925.dir/4925.PDF

SINTITUL-4

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