Electrophoretic proteinogram reference interval from Argentina Northeastern captive caimans (crocodylia: Alligatoridae)

The purpose of the study was to establish the reference interval for serum electrophoretic proteinogram from captive Northeastern Argentinean caimans, as well as to detect physiological variations attributable to species, sex, age, feeding and year season. Samples from 223 healthy sub-adults, both s...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Coppo, J.A., Mussart, N.B., Barboza, N.N., Zeinsteger, P.A., Prado, W.S.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. 2006
Materias:
Caiman latirostris
Caiman yacare
proteinograma electroforético
intervalo de referencia
variaciones fisiológicas
electrophoretic proteinogram
reference interval
physiological variations
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4682
http://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4682.dir/4682.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_4682
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Inglés
orig_language_str_mv eng
topic Caiman latirostris
Caiman yacare
proteinograma electroforético
intervalo de referencia
variaciones fisiológicas
Caiman latirostris
Caiman yacare
electrophoretic proteinogram
reference interval
physiological variations
spellingShingle Caiman latirostris
Caiman yacare
proteinograma electroforético
intervalo de referencia
variaciones fisiológicas
Caiman latirostris
Caiman yacare
electrophoretic proteinogram
reference interval
physiological variations
Coppo, J.A.
Mussart, N.B.
Barboza, N.N.
Zeinsteger, P.A.
Prado, W.S.
Electrophoretic proteinogram reference interval from Argentina Northeastern captive caimans (crocodylia: Alligatoridae)
topic_facet Caiman latirostris
Caiman yacare
proteinograma electroforético
intervalo de referencia
variaciones fisiológicas
Caiman latirostris
Caiman yacare
electrophoretic proteinogram
reference interval
physiological variations
description The purpose of the study was to establish the reference interval for serum electrophoretic proteinogram from captive Northeastern Argentinean caimans, as well as to detect physiological variations attributable to species, sex, age, feeding and year season. Samples from 223 healthy sub-adults, both sexes specimens of Caiman latirostris (n=109) and Caiman yacare (n=114), were obtained. Total protein (4.01±0.61 g/dl) was determined by spectrophotometry. Values from albumin (0.96±0.20 g/dl) and globulins alpha (0.74±0.16 g/dl), beta (0.87±0.18 g/dl) and gamma (1.39±0.27 g/dl), were obtained by electrophoresis on cellulose acetate and densitometric valuation. Presence of pre-albumin on band comprised between 20-66 kDa, were detected by electrophoresis on polyacrilamide gel. Reptile albumin registered molecular weight from 69 kDa. Protein values were higher in males than in females, being significantly higher (p<0.05) for gamma globulin. All the studied parameters were lower in C. latirostris than in C. yacare. Growing produced significant increase of total protein, albumin, and albumin/globulin ratio. On the contrary, these parameters were lower during winter. Handling and feeding systems used with farmed reptiles caused total protein and albumin values higher than those registered in zoo specimens. Obtained data are applicable for caiman?s nutritional control, as well as for diagnosis and illness prevention.
format Artículo
Artículo
publishedVersion
author Coppo, J.A.
Mussart, N.B.
Barboza, N.N.
Zeinsteger, P.A.
Prado, W.S.
author_facet Coppo, J.A.
Mussart, N.B.
Barboza, N.N.
Zeinsteger, P.A.
Prado, W.S.
author_sort Coppo, J.A.
title Electrophoretic proteinogram reference interval from Argentina Northeastern captive caimans (crocodylia: Alligatoridae)
title_short Electrophoretic proteinogram reference interval from Argentina Northeastern captive caimans (crocodylia: Alligatoridae)
title_full Electrophoretic proteinogram reference interval from Argentina Northeastern captive caimans (crocodylia: Alligatoridae)
title_fullStr Electrophoretic proteinogram reference interval from Argentina Northeastern captive caimans (crocodylia: Alligatoridae)
title_full_unstemmed Electrophoretic proteinogram reference interval from Argentina Northeastern captive caimans (crocodylia: Alligatoridae)
title_sort electrophoretic proteinogram reference interval from argentina northeastern captive caimans (crocodylia: alligatoridae)
publisher Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
publishDate 2006
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4682
http://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4682.dir/4682.PDF
work_keys_str_mv AT coppoja electrophoreticproteinogramreferenceintervalfromargentinanortheasterncaptivecaimanscrocodyliaalligatoridae
AT mussartnb electrophoreticproteinogramreferenceintervalfromargentinanortheasterncaptivecaimanscrocodyliaalligatoridae
AT barbozann electrophoreticproteinogramreferenceintervalfromargentinanortheasterncaptivecaimanscrocodyliaalligatoridae
AT zeinstegerpa electrophoreticproteinogramreferenceintervalfromargentinanortheasterncaptivecaimanscrocodyliaalligatoridae
AT pradows electrophoreticproteinogramreferenceintervalfromargentinanortheasterncaptivecaimanscrocodyliaalligatoridae
AT coppoja intervalodereferenciaparaelproteinogramaelectroforeticodecaimanesdelnordesteargentinoencautiveriocrocodyliaalligatoridae
AT mussartnb intervalodereferenciaparaelproteinogramaelectroforeticodecaimanesdelnordesteargentinoencautiveriocrocodyliaalligatoridae
AT barbozann intervalodereferenciaparaelproteinogramaelectroforeticodecaimanesdelnordesteargentinoencautiveriocrocodyliaalligatoridae
AT zeinstegerpa intervalodereferenciaparaelproteinogramaelectroforeticodecaimanesdelnordesteargentinoencautiveriocrocodyliaalligatoridae
AT pradows intervalodereferenciaparaelproteinogramaelectroforeticodecaimanesdelnordesteargentinoencautiveriocrocodyliaalligatoridae
_version_ 1766022760293728256
spelling I28-R145-HWA_46822021-07-15 The purpose of the study was to establish the reference interval for serum electrophoretic proteinogram from captive Northeastern Argentinean caimans, as well as to detect physiological variations attributable to species, sex, age, feeding and year season. Samples from 223 healthy sub-adults, both sexes specimens of Caiman latirostris (n=109) and Caiman yacare (n=114), were obtained. Total protein (4.01±0.61 g/dl) was determined by spectrophotometry. Values from albumin (0.96±0.20 g/dl) and globulins alpha (0.74±0.16 g/dl), beta (0.87±0.18 g/dl) and gamma (1.39±0.27 g/dl), were obtained by electrophoresis on cellulose acetate and densitometric valuation. Presence of pre-albumin on band comprised between 20-66 kDa, were detected by electrophoresis on polyacrilamide gel. Reptile albumin registered molecular weight from 69 kDa. Protein values were higher in males than in females, being significantly higher (p<0.05) for gamma globulin. All the studied parameters were lower in C. latirostris than in C. yacare. Growing produced significant increase of total protein, albumin, and albumin/globulin ratio. On the contrary, these parameters were lower during winter. Handling and feeding systems used with farmed reptiles caused total protein and albumin values higher than those registered in zoo specimens. Obtained data are applicable for caiman?s nutritional control, as well as for diagnosis and illness prevention. Fil: Coppo, J.A. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Cátedra de Fisiología. Corrientes, Argentina Fil: Mussart, N.B. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Cátedra de Fisiología. Corrientes, Argentina Fil: Barboza, N.N. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Cátedra de Fisiología. Corrientes, Argentina Fil: Zeinsteger, P.A. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Cátedra de Fisiología. Corrientes, Argentina Fil: Prado, W.S. Fundación Vida Silvestre Argentina. Refugio «El Cachapé». Chaco, Argentina Coppo, J.A. Mussart, N.B. Barboza, N.N. Zeinsteger, P.A. Prado, W.S. 2006 El propósito del estudio fue establecer el intervalo de referencia para el proteinograma electroforético sérico de caimanes argentinos en cautiverio, así como detectar variaciones fisiológicas atribuibles a especie, sexo, edad, alimentación y estación del año. Se emplearon 223 ejemplares sanos, sub-adultos de ambos sexos, de las especies Caiman latirostris (n=109) y Caiman yacare (n=114). Las proteínas totales (4,01±0,61 g/dl) fueron determinadas por espectrofotometría. Mediante electroforesis en acetato de celulosa y valoración densitométrica, se obtuvieron valores para albúminas (0,96±0,20 g/dl) y globulinas alfa (0,74±0,16 g/dl), beta (0,87±0,18 g/dl) y gamma (1,39±0,27 g/dl). Por electroforesis en gel de poliacrilamida se verificaron los pesos moleculares de pre-albúminas (20-66 kDa) y albúminas (69 kDa). Los valores proteicos fueron mayores en machos que en hembras, significativamente para gamma globulinas (p<0,05). Todos los parámetros fueron más bajos en C. latirostris que en C. yacare. El crecimiento produjo aumentos significativos de proteínas totales, albúminas y relación albúminas/globulinas, parámetros que resultaron más bajos en invierno. Los sistemas de manejo y alimentación aplicados en un criadero produjeron valores de proteínas totales y albúminas superiores a los registrados en un zoológico. Los datos obtenidos son aplicables al control nutricional, así como al diagnóstico y prevención de enfermedades de estos reptiles. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) Caiman latirostris Caiman yacare proteinograma electroforético intervalo de referencia variaciones fisiológicas Caiman latirostris Caiman yacare electrophoretic proteinogram reference interval physiological variations eng Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 08, nº1 Electrophoretic proteinogram reference interval from Argentina Northeastern captive caimans (crocodylia: Alligatoridae) Intervalo de referencia para el proteinograma electroforético de caimanes del nordeste argentino en cautiverio (Crocodylia: Alligatoridae) info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4682 http://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4682.dir/4682.PDF

Ejemplares similares