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Proteins present in an extract of Echinococcus granulosus germinal layer were separated in hydrophobic and hydrophilic phases using Triton X-114. The characterization carried out by sodium dodecyl sulphate polyacrylamide gel electrophoresis showed that proteins of apparent molecular weights of 102,...

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Autores principales:	Prieto, L.H., Fernández, E.D.
Formato:	Artículo publishedVersion
Lenguaje:	Español
Publicado:	Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. 2006
Materias:	Echinococcus granulosus membrana germinal proteínas actividad antigénica germinal layer proteins antigenic activity
Acceso en línea:	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4675 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4675.dir/4675.PDF
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

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spelling	I28-R145-HWA_46752024-11-05 prieto.pmd Proteins present in an extract of Echinococcus granulosus germinal layer were separated in hydrophobic and hydrophilic phases using Triton X-114. The characterization carried out by sodium dodecyl sulphate polyacrylamide gel electrophoresis showed that proteins of apparent molecular weights of 102, 45, 28.5-28 and 15.5-15 kDa were present in the extract and in the aqueous phase (hydrophilic proteins). The immunological characterization of extract proteins performed by immunoblotting against to no immunized sheep serum, against to immunized with E. granulosus antigens sheep serum and against to anti-sheep immunoglobulin serum, showed similar reaction patterns. The dot immunobinding assay of extract proteins after Triton X-114 treatment against to human sera incubated in the presence of sodium dodecil sulphate ionic detergent, showed a positive reaction to cystic echinococcosis human sera. On the other hand, no reaction was observed to human sera without cystic echinococcosis and Mansonella ozzardi human infection serum. Fil: Prieto, L.H. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales. Chubut, Argentina Fil: Fernández, E.D. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales. Cátedra de Química Biológica. Chubut, Argentina Fil: Fernández, E.D. Hospital Zonal Trelew. Laboratorio de Bacteriología. Chubut, Argentina Prieto, L.H. Fernández, E.D. 2006 Proteínas presentes en un extracto de membrana germinal de Echinococcus granulosus fueron separadas en una fase acuosa y en una fase detergente mediante el tratamiento con Triton X-114. La caracterización mediante electroforesis en gel de poliacrilamida en presencia de dodecil sulfato de sodio, mostró que proteínas con pesos moleculares aproximados de 102, 45, 28.5-28 y 15.5-15 kDa se presentaron en el extracto y en la fase acuosa (proteínas hidrofílicas). La caracterización inmunológica de proteínas del extracto mediante inmunotransferencia frente a suero ovino no inmunizado, frente a suero ovino inmunizado con antígenos de E. granulosus y frente a suero anti-inmunoglobulina ovina, mostró patrones de reactividad similares. El ensayo inmunodetección en un punto, de proteínas del extracto previamente tratadas con Triton X-114 frente a sueros humanos incubados con el detergente iónico dodecil sulfato de sodio, mostró reactividad frente a sueros humanos con equinococosis quística y no mostró reactividad frente a sueros humanos sin equinococosis quística ni frente a un suero humano infectado con la filaria Mansonella ozzardi. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) Echinococcus granulosus membrana germinal proteínas actividad antigénica Echinococcus granulosus germinal layer proteins antigenic activity spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 08, nº1 Caracterización y análisis de la actividad antigénica de proteínas de la membrana germinal de Echinococcus granulosus Characterization and antigenic activity of Echinococcus granulosus germinal layer proteins info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4675 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4675.dir/4675.PDF
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
language	Español
orig_language_str_mv	spa
topic	Echinococcus granulosus membrana germinal proteínas actividad antigénica Echinococcus granulosus germinal layer proteins antigenic activity
spellingShingle	Echinococcus granulosus membrana germinal proteínas actividad antigénica Echinococcus granulosus germinal layer proteins antigenic activity Prieto, L.H. Fernández, E.D. prieto.pmd
topic_facet	Echinococcus granulosus membrana germinal proteínas actividad antigénica Echinococcus granulosus germinal layer proteins antigenic activity
description	Proteins present in an extract of Echinococcus granulosus germinal layer were separated in hydrophobic and hydrophilic phases using Triton X-114. The characterization carried out by sodium dodecyl sulphate polyacrylamide gel electrophoresis showed that proteins of apparent molecular weights of 102, 45, 28.5-28 and 15.5-15 kDa were present in the extract and in the aqueous phase (hydrophilic proteins). The immunological characterization of extract proteins performed by immunoblotting against to no immunized sheep serum, against to immunized with E. granulosus antigens sheep serum and against to anti-sheep immunoglobulin serum, showed similar reaction patterns. The dot immunobinding assay of extract proteins after Triton X-114 treatment against to human sera incubated in the presence of sodium dodecil sulphate ionic detergent, showed a positive reaction to cystic echinococcosis human sera. On the other hand, no reaction was observed to human sera without cystic echinococcosis and Mansonella ozzardi human infection serum.
format	Artículo Artículo publishedVersion
author	Prieto, L.H. Fernández, E.D.
author_facet	Prieto, L.H. Fernández, E.D.
author_sort	Prieto, L.H.
title	prieto.pmd
title_short	prieto.pmd
title_full	prieto.pmd
title_fullStr	prieto.pmd
title_full_unstemmed	prieto.pmd
title_sort	prieto.pmd
publisher	Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
publishDate	2006
url	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4675 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4675.dir/4675.PDF
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prieto.pmd

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