oriani2.p65

An investigation was carried out to measure the in vitro susceptibility of opportunistic mycobacteria to three antimicrobial agents. Field strains of nontuberculous mycobacteria were obtained of soil samples from La Pampa province (Argentina). Strains of the following species were tested: Mycobacter...

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Autores principales:	Oriani, D.S., Sagardoy, M.A.
Formato:	Artículo publishedVersion
Lenguaje:	Español
Publicado:	Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. 2005
Materias:	micobacterias atípicas agentes antimicrobianos atypical mycobacteria antimicrobial agents
Acceso en línea:	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4596 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4596.dir/4596.PDF
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-HWA_4596
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spelling	I28-R145-HWA_45962024-11-05 oriani2.p65 An investigation was carried out to measure the in vitro susceptibility of opportunistic mycobacteria to three antimicrobial agents. Field strains of nontuberculous mycobacteria were obtained of soil samples from La Pampa province (Argentina). Strains of the following species were tested: Mycobacterium fortuitum, M. phlei and M. kansasii. The antimicrobial agents were: 2% and 4% formaldehyde solutions, 3% and 5% phenol solutions and 70% alcohol solution. At 20o C, suspensions of the test strains were exposed to the germicides and samples were taken at defined intervals (25 min and 45 min) to determine the concentration of survivors. From these data, the decimal reduction times (D) were calculated for each test strain. The results obtained indicated that the nontuberculous mycobacteria were resistant to formaldehyde 2% during 25 min. The D values found revealed considerable differences in the chemical susceptibilities of the test strains. However, all the tested strains were susceptible to formaldehyde 4%, phenol solutions, and alcohol solution 70%. It is necessary to continue evaluating other germicide agents and their effects on Mycobacterium bovis in order to find effective disinfectants for animal waters and field facilities, thus contributing to the control of tuberculosis in cattle. Fil: Oriani, D.S. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. La Pampa, Argentina Fil: Sagardoy, M.A. Universidad Nacional del Sur. Departamento de Agronomía. Buenos Aires, Argentina Oriani, D.S. Sagardoy, M.A. 2005 El objetivo de este trabajo fue evaluar la susceptibilidad in vitro de nueve micobacterias no tuberculosas frente a tres agentes antimicrobianos de amplio uso. Las micobacterias fueron aisladas de muestras de suelos de la provincia de La Pampa (Argentina). Se utilizaron tres especies ambientales (Mycobacterium fortuitum, M. phlei y M. kansasii), tres agentes químicos (formaldehído 2% y 4 %, fenol 3% y 5% y alcohol 70%) y dos tiempos de exposición (25 min y 45 min). Las micobacterias eran sólo resistentes al formaldehído 2% después de 25 min de exposición. Los valores de reducción decimal (D) calculados con ese tratamiento mostraron que las cepas tenían grandes diferencias de susceptibilidad al formaldehído 2%. Sin embargo, todas las micobacterias fueron eliminadas cuando se usó formaldehído 4%, fenol 3% y 5 % y alcohol 70% (25 min). Es necesario continuar valorando otros agentes germicidas y sus efectos frente a Mycobacterium bovis con el objeto de encontrar desinfectantes efectivos para instalaciones y aguadas, contribuyendo de esta forma en el control de la tuberculosis de los rodeos. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) micobacterias atípicas agentes antimicrobianos atypical mycobacteria antimicrobial agents spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 07, nº1 Susceptibilidad de Mycobacterium fortuitum, Mycobacteriun phlei y Mycobacteriun kansasii frente a tres soluciones germicidas Susceptibility of Mycobacterium fortuitum, Mycobacterium phlei and Mycobacterium kansasii to three different germicide solutions info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4596 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4596.dir/4596.PDF
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
language	Español
orig_language_str_mv	spa
topic	micobacterias atípicas agentes antimicrobianos atypical mycobacteria antimicrobial agents
spellingShingle	micobacterias atípicas agentes antimicrobianos atypical mycobacteria antimicrobial agents Oriani, D.S. Sagardoy, M.A. oriani2.p65
topic_facet	micobacterias atípicas agentes antimicrobianos atypical mycobacteria antimicrobial agents
description	An investigation was carried out to measure the in vitro susceptibility of opportunistic mycobacteria to three antimicrobial agents. Field strains of nontuberculous mycobacteria were obtained of soil samples from La Pampa province (Argentina). Strains of the following species were tested: Mycobacterium fortuitum, M. phlei and M. kansasii. The antimicrobial agents were: 2% and 4% formaldehyde solutions, 3% and 5% phenol solutions and 70% alcohol solution. At 20o C, suspensions of the test strains were exposed to the germicides and samples were taken at defined intervals (25 min and 45 min) to determine the concentration of survivors. From these data, the decimal reduction times (D) were calculated for each test strain. The results obtained indicated that the nontuberculous mycobacteria were resistant to formaldehyde 2% during 25 min. The D values found revealed considerable differences in the chemical susceptibilities of the test strains. However, all the tested strains were susceptible to formaldehyde 4%, phenol solutions, and alcohol solution 70%. It is necessary to continue evaluating other germicide agents and their effects on Mycobacterium bovis in order to find effective disinfectants for animal waters and field facilities, thus contributing to the control of tuberculosis in cattle.
format	Artículo Artículo publishedVersion
author	Oriani, D.S. Sagardoy, M.A.
author_facet	Oriani, D.S. Sagardoy, M.A.
author_sort	Oriani, D.S.
title	oriani2.p65
title_short	oriani2.p65
title_full	oriani2.p65
title_fullStr	oriani2.p65
title_full_unstemmed	oriani2.p65
title_sort	oriani2.p65
publisher	Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
publishDate	2005
url	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_4596 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_4596.dir/4596.PDF
work_keys_str_mv	AT orianids oriani2p65 AT sagardoyma oriani2p65 AT orianids susceptibilidaddemycobacteriumfortuitummycobacteriunphleiymycobacteriunkansasiifrenteatressolucionesgermicidas AT sagardoyma susceptibilidaddemycobacteriumfortuitummycobacteriunphleiymycobacteriunkansasiifrenteatressolucionesgermicidas AT orianids susceptibilityofmycobacteriumfortuitummycobacteriumphleiandmycobacteriumkansasiitothreedifferentgermicidesolutions AT sagardoyma susceptibilityofmycobacteriumfortuitummycobacteriumphleiandmycobacteriumkansasiitothreedifferentgermicidesolutions
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oriani2.p65

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