legales2011 1.indd
The aim of this work were to determine the spontaneous and induced frequency of transplacental micronucleis in Sprague Dawley rats, demonstrating the clastogenic or aneugenic effects in the fetus through the maternal exposure, and their linking with the genotoxic and reproductive effect. Four experi...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2011
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3817 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3817.dir/3817.PDF |
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I28-R145-HWA_38172024-11-05 legales2011 1.indd The aim of this work were to determine the spontaneous and induced frequency of transplacental micronucleis in Sprague Dawley rats, demonstrating the clastogenic or aneugenic effects in the fetus through the maternal exposure, and their linking with the genotoxic and reproductive effect. Four experimental groups were formed, 1) negative control (mockery), 2) solvent control NaCl (0,9 %), 3) cyclophosphamide to 50 mg/kg, and 4) bleomycin to 30 mg/kg. All the groups were administered the 14, 15 and 16 days of the gestation by intraperitoneal route. 48 h to the last inoculation was preceded to the euthanasia, being obtained the maternal bone marrow and fetal liver samples. Bigger induction of damage was observed in fetal hepatic cells when it was compared with maternal bone marrow cells. It was demonstrated that the cyclophosphamide is able to induce bigger citotoxicity and genotoxicity that the bleomycin in both cellular types, demonstrating the transplacental clastogenic potential of both mutagens linking this genotoxicity assay to the reproduction. These results are used in the new drugs evaluation with antigenotoxic effects by transplacental route. Fil: Arencibia, D.F. Instituto Finlay. La Habana, Cuba Fil: Rosario, L.A. Universidad de La Habana. Instituto de Farmacia y Alimentos. La Habana, Cuba Fil: Suárez, Y.E. Universidad Agraria de La Habana. Mayabeque, Cuba Fil: Delgado, L. Universidad de La Habana. Instituto de Farmacia y Alimentos. Centro de Estudios para las Investigaciones y Evaluaciones Biológicas. La Habana, Cuba Arencibia, D.F. Rosario, L.A. Suárez, Y.E. Delgado, L. 2011 El presente trabajo tuvo como objetivo determinar la frecuencia espontánea e inducida de micronúcleos transplacentarios en ratas Sprague Dawley, demostrando el efecto clastogénico o aneugénico en el feto a través de la exposición materna y su vinculación con el efecto genotóxico y reproductivo. Se formaron 4 grupos experimentales, 1) control negativo (simulacro), 2) control solvente NaCl (0,9%), 3) ciclofosfamida 50 mg/kg, y 4) bleomicina 30 mg/kg. Todos los grupos se administraron por vía intraperitoneal a los días 14, 15 y 16 de la gestación. A las 48 h posteriores a la última inoculación se procedió a realizar la eutanasia obteniéndose muestras de médula ósea materna y de hígado fetal. Se observó mayor inducción de daño en células hepáticas fetales que en médula ósea materna. Se demostró que la ciclofosfamida es capaz de inducir mayor citotoxicidad y genotoxicidad que la bleomicina en ambos tipos celulares, demostrando el poder clastogénico transplacentario de ambos mutágenos vinculando este ensayo de genotoxicidad a la reproducción. Además estos resultados se podrán utilizar en la evaluación de nuevas drogas con carácter antigenotóxico por vía transplacentaria. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) Micronúcleos transplacentarios Ratas Sprague Dawley Genotoxicidad Embarazo Transplacental micronucleus Sprague Dawley rats Genotoxicity Pregnancy spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 13, nº1 Frecuencia espontánea en inducida de micronúcleos transplacentarios en ratas Sprague Dawley Spontaneous and induced frequency of transplacental micronucleus in Sprague Dawley rats info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3817 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3817.dir/3817.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Micronúcleos transplacentarios Ratas Sprague Dawley Genotoxicidad Embarazo Transplacental micronucleus Sprague Dawley rats Genotoxicity Pregnancy |
| spellingShingle |
Micronúcleos transplacentarios Ratas Sprague Dawley Genotoxicidad Embarazo Transplacental micronucleus Sprague Dawley rats Genotoxicity Pregnancy Arencibia, D.F. Rosario, L.A. Suárez, Y.E. Delgado, L. legales2011 1.indd |
| topic_facet |
Micronúcleos transplacentarios Ratas Sprague Dawley Genotoxicidad Embarazo Transplacental micronucleus Sprague Dawley rats Genotoxicity Pregnancy |
| description |
The aim of this work were to determine the spontaneous and induced frequency of transplacental micronucleis in Sprague Dawley rats, demonstrating the clastogenic or aneugenic effects in the fetus through the maternal exposure, and their linking with the genotoxic and reproductive effect. Four experimental groups were formed, 1) negative control (mockery), 2) solvent control NaCl (0,9 %), 3) cyclophosphamide to 50 mg/kg, and 4) bleomycin to 30 mg/kg. All the groups were administered the 14, 15 and 16 days of the gestation by intraperitoneal route. 48 h to the last inoculation was preceded to the euthanasia, being obtained the maternal bone marrow and fetal liver samples. Bigger induction of damage was observed in fetal hepatic cells when it was compared with maternal bone marrow cells. It was demonstrated that the cyclophosphamide is able to induce bigger citotoxicity and genotoxicity that the bleomycin in both cellular types, demonstrating the transplacental clastogenic potential of both mutagens linking this genotoxicity assay to the reproduction. These results are used in the new drugs evaluation with antigenotoxic effects by transplacental route. |
| format |
Artículo Artículo publishedVersion |
| author |
Arencibia, D.F. Rosario, L.A. Suárez, Y.E. Delgado, L. |
| author_facet |
Arencibia, D.F. Rosario, L.A. Suárez, Y.E. Delgado, L. |
| author_sort |
Arencibia, D.F. |
| title |
legales2011 1.indd |
| title_short |
legales2011 1.indd |
| title_full |
legales2011 1.indd |
| title_fullStr |
legales2011 1.indd |
| title_full_unstemmed |
legales2011 1.indd |
| title_sort |
legales2011 1.indd |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2011 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3817 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3817.dir/3817.PDF |
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