InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in...

Bovine herpesvirus type 1 (BoHV-1) is the etiological agent of Infectious Bovine Rhinotracheitis (IBR). Depending on the genotype, it produces a wide variety of clinical syndromes, such as conjunctivitis, abortion and balanoposthitis. Vaccination against BoHV-1 is a common practice, so in order to d...

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Detalles Bibliográficos
Autores principales: Puentes, R., Llambí, S., Andrés Iriarte, A., Furtado, A., Franco, G., Maisonnave, J., Cristina, J., Murakami, K., Esteves, P.A.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. 2012
Materias:
UL49
VP22
Herpesvirus Bovino
Uruguay
Variability
BoHV isolates
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3807
https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3807.dir/3807.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Bovine herpesvirus type 1 (BoHV-1) is the etiological agent of Infectious Bovine Rhinotracheitis (IBR). Depending on the genotype, it produces a wide variety of clinical syndromes, such as conjunctivitis, abortion and balanoposthitis. Vaccination against BoHV-1 is a common practice, so in order to differentiate infected from vaccinated animals, many BoHV-1 proteins were studied to develop marked vaccines. VP22, the major tegument protein codified by UL49 gene, is a non essential protein for virus multiplication, but induces a relevant immune response in the host. Therefore the variability of the gene UL49 of three BoHV-1 Uruguayan isolates (Uy1999, Uy2002 and Uy2004), was analyzed. Recombinant VP22 was expressed in a prokaryotic system and was recognized by sera of BoHV-1 immunized rabbits by Western blot analysis. Uy1999 (BoHV-1.1), Uy2002 and Uy2004 (BoHV-1.2), have 93.8%, 95% and 88.2% nucleotide similarity to the international Los Angeles BoHV-1.1 reference strain. The phylogenetic analysis showed that Uy1999 is grouped with BoHV-1.1 strains, while Uy2002 and Uy2004 isolates are grouped distant from other BoHV-1.1. The use of VP22 deleted vaccines and a serological technique able to detect antibodies against it, is an excellent alternative to be used in bovine vaccination programmes.

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