InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in...
Bovine herpesvirus type 1 (BoHV-1) is the etiological agent of Infectious Bovine Rhinotracheitis (IBR). Depending on the genotype, it produces a wide variety of clinical syndromes, such as conjunctivitis, abortion and balanoposthitis. Vaccination against BoHV-1 is a common practice, so in order to d...
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| Autores principales: | , , , , , , , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2012
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3807 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3807.dir/3807.PDF |
| Aporte de: |
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I28-R145-HWA_3807 |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
UL49 VP22 Herpesvirus Bovino Uruguay UL49 Variability BoHV isolates Uruguay |
| spellingShingle |
UL49 VP22 Herpesvirus Bovino Uruguay UL49 Variability BoHV isolates Uruguay Puentes, R. Llambí, S. Andrés Iriarte, A. Furtado, A. Franco, G. Maisonnave, J. Cristina, J. Murakami, K. Esteves, P.A. InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in... |
| topic_facet |
UL49 VP22 Herpesvirus Bovino Uruguay UL49 Variability BoHV isolates Uruguay |
| description |
Bovine herpesvirus type 1 (BoHV-1) is the etiological agent of Infectious Bovine Rhinotracheitis (IBR). Depending on the genotype, it produces a wide variety of clinical syndromes, such as conjunctivitis, abortion and balanoposthitis. Vaccination against BoHV-1 is a common practice, so in order to differentiate infected from vaccinated animals, many BoHV-1 proteins were studied to develop marked vaccines. VP22, the major tegument protein codified by UL49 gene, is a non essential protein for virus multiplication, but induces a relevant immune response in the host. Therefore the variability of the gene UL49 of three BoHV-1 Uruguayan isolates (Uy1999, Uy2002 and Uy2004), was analyzed. Recombinant VP22 was expressed in a prokaryotic system and was recognized by sera of BoHV-1 immunized rabbits by Western blot analysis. Uy1999 (BoHV-1.1), Uy2002 and Uy2004 (BoHV-1.2), have 93.8%, 95% and 88.2% nucleotide similarity to the international Los Angeles BoHV-1.1 reference strain. The phylogenetic analysis showed that Uy1999 is grouped with BoHV-1.1 strains, while Uy2002 and Uy2004 isolates are grouped distant from other BoHV-1.1. The use of VP22 deleted vaccines and a serological technique able to detect antibodies against it, is an excellent alternative to be used in bovine vaccination programmes. |
| format |
Artículo Artículo publishedVersion |
| author |
Puentes, R. Llambí, S. Andrés Iriarte, A. Furtado, A. Franco, G. Maisonnave, J. Cristina, J. Murakami, K. Esteves, P.A. |
| author_facet |
Puentes, R. Llambí, S. Andrés Iriarte, A. Furtado, A. Franco, G. Maisonnave, J. Cristina, J. Murakami, K. Esteves, P.A. |
| author_sort |
Puentes, R. |
| title |
InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in... |
| title_short |
InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in... |
| title_full |
InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in... |
| title_fullStr |
InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in... |
| title_full_unstemmed |
InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in... |
| title_sort |
invet. 2012, 14(2): 163-171 issn 1514-6634 (impreso) variability of ul49 in... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2012 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3807 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3807.dir/3807.PDF |
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I28-R145-HWA_38072024-11-05 InVet. 2012, 14(2): 163-171 ISSN 1514-6634 (impreso) Variability of Ul49 in... Bovine herpesvirus type 1 (BoHV-1) is the etiological agent of Infectious Bovine Rhinotracheitis (IBR). Depending on the genotype, it produces a wide variety of clinical syndromes, such as conjunctivitis, abortion and balanoposthitis. Vaccination against BoHV-1 is a common practice, so in order to differentiate infected from vaccinated animals, many BoHV-1 proteins were studied to develop marked vaccines. VP22, the major tegument protein codified by UL49 gene, is a non essential protein for virus multiplication, but induces a relevant immune response in the host. Therefore the variability of the gene UL49 of three BoHV-1 Uruguayan isolates (Uy1999, Uy2002 and Uy2004), was analyzed. Recombinant VP22 was expressed in a prokaryotic system and was recognized by sera of BoHV-1 immunized rabbits by Western blot analysis. Uy1999 (BoHV-1.1), Uy2002 and Uy2004 (BoHV-1.2), have 93.8%, 95% and 88.2% nucleotide similarity to the international Los Angeles BoHV-1.1 reference strain. The phylogenetic analysis showed that Uy1999 is grouped with BoHV-1.1 strains, while Uy2002 and Uy2004 isolates are grouped distant from other BoHV-1.1. The use of VP22 deleted vaccines and a serological technique able to detect antibodies against it, is an excellent alternative to be used in bovine vaccination programmes. Fil: Puentes, R. Universidad de la República. Facultad de Veterinaria. Departamento de Ciencias Microbiológicas. Montevideo, Uruguay Fil: Llambí, S. Universidad de la República. Facultad de Veterinaria. Area de Genética. Montevideo, Uruguay Fil: Andrés Iriarte, A. Universidad de la República. Facultad de Veterinaria. Area de Genética. Montevideo, Uruguay Fil: Furtado, A. Universidad de la República. Facultad de Veterinaria. Departamento de Ciencias Microbiológicas. Montevideo, Uruguay Fil: Franco, G. Universidad de la República. Facultad de Veterinaria. Departamento de Ciencias Microbiológicas. Montevideo, Uruguay Fil: Maisonnave, J. Universidad de la República. Facultad de Veterinaria. Departamento de Ciencias Microbiológicas. Montevideo, Uruguay Fil: Cristina, J. Universidad de la República. Facultad de Veterinaria. Centro de Investigaciones Nucleares. Montevideo, Uruguay Fil: Murakami, K. National Institute of Animal Health. Kannondai, Japan Fil: Esteves, P.A. Empresa Brasileira de Pesquisa Agropecuária. Concórdia, Santa Catarina, Brasil Puentes, R. Llambí, S. Andrés Iriarte, A. Furtado, A. Franco, G. Maisonnave, J. Cristina, J. Murakami, K. Esteves, P.A. 2012 Herpesvirus bovino tipo 1 (BoHV-1) es el agente responsable de la Rinotraqueitis Infecciosa Bovina (IBR) y de un amplia variedad de síndromes, como conjuntivitis, aborto y balanopostitis, según el genotipo que se encuentre presente. La vacunación contra BoHV-1 es una tarea de rutina en el manejo de ganado, por lo tanto se han estudiado varias proteínas de BoHV-1 con el objetivo de desarrollar vacunas marcadas que permitan la diferenciación entre animales vacunados e infectados. VP22 es la proteína principal del tegumento del virus y es codificada por el gen UL49. No es esencial para el ciclo de replicación viral pero induce una respuesta inmune significativa en el huésped. Por consiguiente se analizó la variabilidad del gen UL49 en tres aislamientos uruguayos de BoHV-1 (Uy1999, Uy2002 y Uy2004), se expresó una VP22 recombinante que fue reconocida por sueros de conejos inmunizados con BoHV-1 mediante su análisis por Western Blot. Los resultados obtenidos muestran que Uy1999 (BoHV-1.1), Uy2002 y Uy2004 (BoHV-1.2), tienen 93.8%, 95% y 88.2% de similitud en la secuencia de nucleótidos al compararlos con la cepa de referencia internacional Los Ángeles BoHV-1.1. El análisis filogenético mostró que Uy1999 está agrupado con cepas BoHV-1.1, mientras que Uy2002 y Uy2004 están agrupadas distantes de los otros BoHV-1.1. El uso conjunto de vacunas a las cuales se les ha eliminado VP22 y de técnicas capaces de detectar anticuerpos contra la proteína eliminada es una excelente alternativa a usar en los programas de vacunación bovina. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) UL49 VP22 Herpesvirus Bovino Uruguay UL49 Variability BoHV isolates Uruguay eng Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 14, nº2 Variability of UL49 in bovine herpesvirus type 1 (BoHV-1) from Uruguayan isolates Variabilidad de UL49 en herpesvirus bovino tipo 1 (BoHV-1) en aislamientos de Uruguay info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3807 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3807.dir/3807.PDF |