InVet. 2020, 22 (1): 33-40 POLIMORFISMO ISSN 1514-6634 (impreso) DEL GEN...
The WDTC1 (WD and tetratricopeptide repeats 1) gene has an anti-adipogenic function, converting it into a candidate gene to explain the phenotypic variation in traits of economic importance for swine breeding. The objective of this work was to identify and evaluate the effect of single nucleotide po...
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| Autores principales: | , , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2020
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3711 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3711.dir/3711.PDF |
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I28-R145-HWA_37112024-11-05 InVet. 2020, 22 (1): 33-40 POLIMORFISMO ISSN 1514-6634 (impreso) DEL GEN... The WDTC1 (WD and tetratricopeptide repeats 1) gene has an anti-adipogenic function, converting it into a candidate gene to explain the phenotypic variation in traits of economic importance for swine breeding. The objective of this work was to identify and evaluate the effect of single nucleotide polymorphisms (SNPs) located in the 5`UTR region of WDTC1 and its association with backfat thickness (BFT) in 128 Landrace pigs. For the identification of SNPs, 808 bp (from 84,466,928 to 84,467,736 of sequence NC010448.4 of GenBank) were amplified, sequenced and analyzed by multiple alignments. The polymorphisms found were: SNP1 (T / C, 84.466.982), SNP2 (C / T, 84.467.066), SNP3 (T / C 84.467.131), SNP4 (C / T, 84.467.201), SNP5 (G / T, 84.467.368), SNP6 (C / A, 84.467.402) and SNP7 (G / A, 84.467.413). Statistical analysis was performed using ANOVA only for the SNP1. The differences were significant among genotypes of SNP 1 (p-value <0.05) for (BFT). These results indicate that the 5' UTR region of WDTC1 is polymorph and the SNP1 would be associated with the phenotypic expression of the character analyzed. Fil: Fassa, M.B. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Genética. Buenos Aires, Argentina Fil: Schor, A. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra Bovinos de Carne. Buenos Aires, Argentina Fil: Lloveras, M.R. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino (EEA). Buenos Aires, Argentina Fil: Marrube, G. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Genética. Buenos Aires, Argentina Fassa, M.B. Schor, A. Lloveras, M.R. Marrube, G. 2020 El gen WDTC1 (WD tetratricopeptido 1) tiene una función anti-adipogénica, convirtiéndose de esta manera en un gen candidato para explicar la variación fenotípica en caracteres de importancia económica de la producción porcina. El objetivo de este trabajo fue identificar polimorfismos de nucleótidos simples (SNP) en la región 5´ no traducida (UTR) del gen WDTC1 y evaluar su asociación con el espesor de grasa dorsal (EGD) en 128 cerdos de raza Landrace. Para la búsqueda de los SNPs se amplificaron y secuenciaron 808 pares de bases (pb) (posición 84.466.928 a 84.467.736 de la secuencia NC010448.4 del GenBank) en 48 cerdos, hallándose siete nuevos SNPs. Los polimorfismos hallados fueron: (i) SNP1 (T/C, posición 84.466.982); (ii) SNP2 (C/T, posición 84.467.066); (iii) SNP3 (T/C posición 84.467.131); (iv) SNP4 (C/T, posición 84.467.201); (v) SNP5 (G/T, posición 84.467.368); (vi) SNP6 (C/A, posición 84.467.402); y (vii) SNP7 (G/A, posición 84.467.413). El análisis estadístico se realizó mediante ANOVA solamente para el SNP1. Se encontraron diferencias significativas (p<0,05) en 128 cerdos, entre genotipos del SNP1 y EGD. Los resultados obtenidos sugieren que la región 5´UTR de WDTC1 es polimórfica y el SNP1 estaría asociado con la expresión fenotípica del carácter. application/pdf 1514-6634 (impreso) 1668-3498 (en l?nea) Porcinos Espesor de grasa dorsal Gen WDTC1 Porcine Backfat thickness WDTC1 gene spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 22, nº1 Polimorfismos en el extremo 5´ del gen WDTC1 y su asociación con el espesor de grasa dorsal en cerdos Landrace Polymorphysms in the 5´ end of WDTC1 gene and its association with backfat thickness in Landrace pigs info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3711 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3711.dir/3711.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
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Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Porcinos Espesor de grasa dorsal Gen WDTC1 Porcine Backfat thickness WDTC1 gene |
| spellingShingle |
Porcinos Espesor de grasa dorsal Gen WDTC1 Porcine Backfat thickness WDTC1 gene Fassa, M.B. Schor, A. Lloveras, M.R. Marrube, G. InVet. 2020, 22 (1): 33-40 POLIMORFISMO ISSN 1514-6634 (impreso) DEL GEN... |
| topic_facet |
Porcinos Espesor de grasa dorsal Gen WDTC1 Porcine Backfat thickness WDTC1 gene |
| description |
The WDTC1 (WD and tetratricopeptide repeats 1) gene has an anti-adipogenic function, converting it into a candidate gene to explain the phenotypic variation in traits of economic importance for swine breeding. The objective of this work was to identify and evaluate the effect of single nucleotide polymorphisms (SNPs) located in the 5`UTR region of WDTC1 and its association with backfat thickness (BFT) in 128 Landrace pigs. For the identification of SNPs, 808 bp (from 84,466,928 to 84,467,736 of sequence NC010448.4 of GenBank) were amplified, sequenced and analyzed by multiple alignments. The polymorphisms found were: SNP1 (T / C, 84.466.982), SNP2 (C / T, 84.467.066), SNP3 (T / C 84.467.131), SNP4 (C / T, 84.467.201), SNP5 (G / T, 84.467.368), SNP6 (C / A, 84.467.402) and SNP7 (G / A, 84.467.413). Statistical analysis was performed using ANOVA only for the SNP1. The differences were significant among genotypes of SNP 1 (p-value <0.05) for (BFT). These results indicate that the 5' UTR region of WDTC1 is polymorph and the SNP1 would be associated with the phenotypic expression of the character analyzed. |
| format |
Artículo Artículo publishedVersion |
| author |
Fassa, M.B. Schor, A. Lloveras, M.R. Marrube, G. |
| author_facet |
Fassa, M.B. Schor, A. Lloveras, M.R. Marrube, G. |
| author_sort |
Fassa, M.B. |
| title |
InVet. 2020, 22 (1): 33-40 POLIMORFISMO ISSN 1514-6634 (impreso) DEL GEN... |
| title_short |
InVet. 2020, 22 (1): 33-40 POLIMORFISMO ISSN 1514-6634 (impreso) DEL GEN... |
| title_full |
InVet. 2020, 22 (1): 33-40 POLIMORFISMO ISSN 1514-6634 (impreso) DEL GEN... |
| title_fullStr |
InVet. 2020, 22 (1): 33-40 POLIMORFISMO ISSN 1514-6634 (impreso) DEL GEN... |
| title_full_unstemmed |
InVet. 2020, 22 (1): 33-40 POLIMORFISMO ISSN 1514-6634 (impreso) DEL GEN... |
| title_sort |
invet. 2020, 22 (1): 33-40 polimorfismo issn 1514-6634 (impreso) del gen... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2020 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3711 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3711.dir/3711.PDF |
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