InVet. 2020, 22 (1): 5-10 ISSN 1514-6634 (impreso) PRUEBA IN VITRO DE...
Cefovecin is a semisynthetic extended-spectrum antimicrobial for veterinary use that belongs to the third generation cephalosporins. It is sold as a lyophilized powder and, once it is reconstituted, the solution has a 28-day expiration conserved at 4ºC. The objective of the present work was to deter...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2020
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3707 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3707.dir/3707.PDF |
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I28-R145-HWA_3707 |
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I28-R145-HWA_37072024-11-05 InVet. 2020, 22 (1): 5-10 ISSN 1514-6634 (impreso) PRUEBA IN VITRO DE... Cefovecin is a semisynthetic extended-spectrum antimicrobial for veterinary use that belongs to the third generation cephalosporins. It is sold as a lyophilized powder and, once it is reconstituted, the solution has a 28-day expiration conserved at 4ºC. The objective of the present work was to determine the presence of modifications over time in the antibacterial potency of the reconstituted and stored cefovecin in three different ways: 4ºC, -20ºC and -70ºC. The first three months after reconstitution, the potency of cefovecin was compared between the conserved dilutions at 4 ºC, -20 ºC and -70 ºC; and, for the fourth to twelfth months after reconstitution, it was compared between conserved dilutions at -20ºC and -70 ºC. The in vitro antimicrobial activity of the antibiotic was evaluated by plaque diffusion microbiological assay. The parameters evaluated (R2, slope and ordered at the origin) were not statistically different (P> 0.05) when they were compared for the different conservation temperatures throughout the entire study. It can be concluded that the reconstituted cefovecin did not lose its antibiotic potency when it was subjected to freezing neither during a period of 12 months nor during the 3 months that it was evaluated preserved in the refrigerator.\n Fil: Doxandabarat, X.D. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Farmacología. Buenos Aires, Argentina Fil: Paes Rodríguez, J.D. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Farmacología. Buenos Aires, Argentina Fil: Albarellos, G.A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Farmacología. Buenos Aires, Argentina Doxandabarat, X.D. Paes Rodríguez, J.D. Albarellos, G.A. 2020 El cefovecin es un antimicrobiano de espectro extendido de uso veterinario que pertenece a la tercera generación de cefalosporinas. Se expende como un liofilizado y la solución reconstituida tiene un vencimiento de 28 días conservado a 4ºC. El objetivo del presente trabajo fue determinar la presencia de modificaciones a lo largo del tiempo en la potencia antibacteriana del cefovecin reconstituido y almacenado de tres formas diferentes: 4 ºC, -20 ºC y -70 ºC. Los tres primeros meses se comparó la potencia de cefovecin conservado a 4 ºC, -20 ºC y -70 ºC, y entre los meses cuarto a duodécimo se comparó a -20 ºC y -70 ºC. La actividad antimicrobiana in vitro del antibiótico se evaluó mediante el método microbiológico de difusión en placa. Los parámetros evaluados (R2, pendiente y ordenada al origen) no fueron estadísticamente diferentes (P>0,05) cuando se compararon para las distintas temperaturas de conservación a lo largo de todo el estudio. Se puede concluir que el cefovecin reconstituido no perdió su potencia antibiótica cuando fue sometido a congelación durante un período de 12 meses ni tampoco durante los 3 meses que se evaluó conservado en heladera. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) Betalactámico Potencia Conservación Antibiótico Cefalosporina Beta-lactams Potency Conservation Antibiotic Cephalosporins spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 22, nº1 Prueba in vitro de potencia de cefovecin luego de su conservación a distintas temperaturas In vitro potency test of cefovecin after its conservation at different temperatures info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3707 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3707.dir/3707.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Betalactámico Potencia Conservación Antibiótico Cefalosporina Beta-lactams Potency Conservation Antibiotic Cephalosporins |
| spellingShingle |
Betalactámico Potencia Conservación Antibiótico Cefalosporina Beta-lactams Potency Conservation Antibiotic Cephalosporins Doxandabarat, X.D. Paes Rodríguez, J.D. Albarellos, G.A. InVet. 2020, 22 (1): 5-10 ISSN 1514-6634 (impreso) PRUEBA IN VITRO DE... |
| topic_facet |
Betalactámico Potencia Conservación Antibiótico Cefalosporina Beta-lactams Potency Conservation Antibiotic Cephalosporins |
| description |
Cefovecin is a semisynthetic extended-spectrum antimicrobial for veterinary use that belongs to the third generation cephalosporins. It is sold as a lyophilized powder and, once it is reconstituted, the solution has a 28-day expiration conserved at 4ºC. The objective of the present work was to determine the presence of modifications over time in the antibacterial potency of the reconstituted and stored cefovecin in three different ways: 4ºC, -20ºC and -70ºC. The first three months after reconstitution, the potency of cefovecin was compared between the conserved dilutions at 4 ºC, -20 ºC and -70 ºC; and, for the fourth to twelfth months after reconstitution, it was compared between conserved dilutions at -20ºC and -70 ºC. The in vitro antimicrobial activity of the antibiotic was evaluated by plaque diffusion microbiological assay. The parameters evaluated (R2, slope and ordered at the origin) were not statistically different (P> 0.05) when they were compared for the different conservation temperatures throughout the entire study. It can be concluded that the reconstituted cefovecin did not lose its antibiotic potency when it was subjected to freezing neither during a period of 12 months nor during the 3 months that it was evaluated preserved in the refrigerator.\n |
| format |
Artículo Artículo publishedVersion |
| author |
Doxandabarat, X.D. Paes Rodríguez, J.D. Albarellos, G.A. |
| author_facet |
Doxandabarat, X.D. Paes Rodríguez, J.D. Albarellos, G.A. |
| author_sort |
Doxandabarat, X.D. |
| title |
InVet. 2020, 22 (1): 5-10 ISSN 1514-6634 (impreso) PRUEBA IN VITRO DE... |
| title_short |
InVet. 2020, 22 (1): 5-10 ISSN 1514-6634 (impreso) PRUEBA IN VITRO DE... |
| title_full |
InVet. 2020, 22 (1): 5-10 ISSN 1514-6634 (impreso) PRUEBA IN VITRO DE... |
| title_fullStr |
InVet. 2020, 22 (1): 5-10 ISSN 1514-6634 (impreso) PRUEBA IN VITRO DE... |
| title_full_unstemmed |
InVet. 2020, 22 (1): 5-10 ISSN 1514-6634 (impreso) PRUEBA IN VITRO DE... |
| title_sort |
invet. 2020, 22 (1): 5-10 issn 1514-6634 (impreso) prueba in vitro de... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2020 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3707 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3707.dir/3707.PDF |
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