InVet. 2019, 21 (1): 5-16 ISSN 1514-6634 (impreso) ANTIOXIDANT METFORMIN...
Hyperandrogenization is one of the main clinical features of the polycystic ovarian syndrome (PCOS). Metformin (M) is a non-hormonal treatment used in PCOS even during pregnancy. The objective was to study the effects of M on the redox balance and the nitric oxide (NO) system in a murine model of ea...
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| Autores principales: | , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2019
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3676 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3676.dir/3676.PDF |
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I28-R145-HWA_3676 |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
DHEA Estrés oxidativo ON Síndrome de ovario poliquístico Sitios de implantación DHEA Oxidative stress NO Polycystic ovary syndrome Implantation sites |
| spellingShingle |
DHEA Estrés oxidativo ON Síndrome de ovario poliquístico Sitios de implantación DHEA Oxidative stress NO Polycystic ovary syndrome Implantation sites Luchetti, C.G. Motta, A.B. Lombardo, D.M. InVet. 2019, 21 (1): 5-16 ISSN 1514-6634 (impreso) ANTIOXIDANT METFORMIN... |
| topic_facet |
DHEA Estrés oxidativo ON Síndrome de ovario poliquístico Sitios de implantación DHEA Oxidative stress NO Polycystic ovary syndrome Implantation sites |
| description |
Hyperandrogenization is one of the main clinical features of the polycystic ovarian syndrome (PCOS). Metformin (M) is a non-hormonal treatment used in PCOS even during pregnancy. The objective was to study the effects of M on the redox balance and the nitric oxide (NO) system in a murine model of early hyperandrogenized pregnancy. Early pregnant Balb/ c mice hyperandrogenized by dehydroepiandrosterone (DHEA) and treated orally with M were used. Redox and NO system parameters were determined at the implantation sites. DHEA increased oxidative stress: lipid peroxidation (TBA-RS, p <0.01) and glutathione (Tietze; p <0.01). With DHEA + M, TBA-RS was similar to the control and glutathione was similar to the DHEA group. The enzymes superoxide dismutase and catalase did not show differences. DHEA caused an increase in the expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) (immunohistochemistry) and in NO (Griess; p <0.001 in all cases). With DHEA+M they were similar to the control. We conclude that M avoids the effects of DHEA on oxidative stress and the NO system in the implantation sites of hyperandrogenized early pregnant Balb/c mice. |
| format |
Artículo Artículo publishedVersion |
| author |
Luchetti, C.G. Motta, A.B. Lombardo, D.M. |
| author_facet |
Luchetti, C.G. Motta, A.B. Lombardo, D.M. |
| author_sort |
Luchetti, C.G. |
| title |
InVet. 2019, 21 (1): 5-16 ISSN 1514-6634 (impreso) ANTIOXIDANT METFORMIN... |
| title_short |
InVet. 2019, 21 (1): 5-16 ISSN 1514-6634 (impreso) ANTIOXIDANT METFORMIN... |
| title_full |
InVet. 2019, 21 (1): 5-16 ISSN 1514-6634 (impreso) ANTIOXIDANT METFORMIN... |
| title_fullStr |
InVet. 2019, 21 (1): 5-16 ISSN 1514-6634 (impreso) ANTIOXIDANT METFORMIN... |
| title_full_unstemmed |
InVet. 2019, 21 (1): 5-16 ISSN 1514-6634 (impreso) ANTIOXIDANT METFORMIN... |
| title_sort |
invet. 2019, 21 (1): 5-16 issn 1514-6634 (impreso) antioxidant metformin... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2019 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3676 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3676.dir/3676.PDF |
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AT luchetticg invet2019211516issn15146634impresoantioxidantmetformin AT mottaab invet2019211516issn15146634impresoantioxidantmetformin AT lombardodm invet2019211516issn15146634impresoantioxidantmetformin AT luchetticg antioxidanteffectofmetformininamurinemodelofhyperandrogenizedpregnancy AT mottaab antioxidanteffectofmetformininamurinemodelofhyperandrogenizedpregnancy AT lombardodm antioxidanteffectofmetformininamurinemodelofhyperandrogenizedpregnancy |
| _version_ |
1824359733425864704 |
| spelling |
I28-R145-HWA_36762024-11-05 InVet. 2019, 21 (1): 5-16 ISSN 1514-6634 (impreso) ANTIOXIDANT METFORMIN... Hyperandrogenization is one of the main clinical features of the polycystic ovarian syndrome (PCOS). Metformin (M) is a non-hormonal treatment used in PCOS even during pregnancy. The objective was to study the effects of M on the redox balance and the nitric oxide (NO) system in a murine model of early hyperandrogenized pregnancy. Early pregnant Balb/ c mice hyperandrogenized by dehydroepiandrosterone (DHEA) and treated orally with M were used. Redox and NO system parameters were determined at the implantation sites. DHEA increased oxidative stress: lipid peroxidation (TBA-RS, p <0.01) and glutathione (Tietze; p <0.01). With DHEA + M, TBA-RS was similar to the control and glutathione was similar to the DHEA group. The enzymes superoxide dismutase and catalase did not show differences. DHEA caused an increase in the expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) (immunohistochemistry) and in NO (Griess; p <0.001 in all cases). With DHEA+M they were similar to the control. We conclude that M avoids the effects of DHEA on oxidative stress and the NO system in the implantation sites of hyperandrogenized early pregnant Balb/c mice. Fil: Luchetti, C.G. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Buenos Aires, Argentina Fil: Luchetti, C.G. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Buenos Aires, Argentina Fil: Motta, A.B. Universidad de Buenos Aires. Facultad de Medicina. Buenos Aires, Argentina Fil: Motta, A.B. CONICET - Universidad de Buenos Aires. Centro de Estudios Farmacológicos y Botánicos (CEFYBO). Buenos Aires, Argentina Fil: Lombardo, D.M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Buenos Aires, Argentina Fil: Lombardo, D.M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Buenos Aires, Argentina Luchetti, C.G. Motta, A.B. Lombardo, D.M. 2019 La hiperandrogenización es una de las características clínicas principales del síndrome de ovario poliquístico (SOP). La metformina (M) es un tratamiento no hormonal utilizado en el SOP incluso durante el embarazo. El objetivo fue estudiar los efectos de M sobre el balance redox y el sistema óxido nítrico (ON) en un modelo murino de preñez temprana hiperandrogenizada. Se utilizaron ratones Balb/ c hiperandrogenizados por dehidroepiandrosterona (DHEA) durante la preñez temprana y tratados oralmente con M. Se determinaron parámetros redox y del sistema ON en los sitios de implantación. La DHEA incrementó el estrés oxidativo: peroxidación lipídica (TBA-RS; p <0.01) y glutatión (Tietze; p <0.01). Con DHEA+M, TBA-RS fue similar al control y el glutatión fue similar al grupo DHEA. Las enzimas superóxido dismutasa y catalasa no mostraron diferencias. La DHEA provocó un aumento en la expresión de óxido nítrico sintetasa inducible (ONSi) y óxido nítrico sintetasa endotelial (ONSe) (inmunohistoquímica) y en ON (Griess; p <0,001 en todos los casos). Con DHEA+M fueron similares al control. Concluimos que M evita los efectos de DHEA sobre el estrés oxidativo y el sistema ON en los sitios de implantación de ratones Balb/ c durante la preñez temprana hiperandrogenizada. application/pdf 1514-6634 (impreso) 1668-3498 (en l?nea) DHEA Estrés oxidativo ON Síndrome de ovario poliquístico Sitios de implantación DHEA Oxidative stress NO Polycystic ovary syndrome Implantation sites eng Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 21, nº1 Antioxidant effect of metformin in a murine model of hyperandrogenized pregnancy info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3676 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3676.dir/3676.PDF |