InVet. 2016, 18 (2): 341-347 ISSN 1514-6634 (impr ANÁLISIS FIL eso) ...
Potomac horse fever (equine monocytic ehrlichiosis) is caused by Neorickettsia risticii (formerly Ehrlichia risticii), with clinical cases reported only in the USA, Brazil and Uruguay. N. risticii is an endosymbiont of flukes with a complex life cycle utilizing various hosts such as aquatic snails a...
| Autores principales: | , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
| Publicado: |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2016
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| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3477 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3477.dir/3477.PDF |
| Aporte de: |
| id |
I28-R145-HWA_3477 |
|---|---|
| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Neorickettsia risticii Ehrlichiosis monocítica equina Fiebre equina del Potomac Murciélagos Argentina Neorickettsia risticii Equine monocytic ehrlichiosis Potomac horse fever Bats Argentina |
| spellingShingle |
Neorickettsia risticii Ehrlichiosis monocítica equina Fiebre equina del Potomac Murciélagos Argentina Neorickettsia risticii Equine monocytic ehrlichiosis Potomac horse fever Bats Argentina Cicuttin, G.L. Greiman, S.E. InVet. 2016, 18 (2): 341-347 ISSN 1514-6634 (impr ANÁLISIS FIL eso) ... |
| topic_facet |
Neorickettsia risticii Ehrlichiosis monocítica equina Fiebre equina del Potomac Murciélagos Argentina Neorickettsia risticii Equine monocytic ehrlichiosis Potomac horse fever Bats Argentina |
| description |
Potomac horse fever (equine monocytic ehrlichiosis) is caused by Neorickettsia risticii (formerly Ehrlichia risticii), with clinical cases reported only in the USA, Brazil and Uruguay. N. risticii is an endosymbiont of flukes with a complex life cycle utilizing various hosts such as aquatic snails and arthropods, and vertebrate insectivores. Different phylogenetic studies revealed that N. risticii consists of various strains in the USA, but no information is available from Brazil and Uruguay. In our country, N. risticii was first detected in 2013 in Tadarida brasiliensis bats from Buenos Aires city\n(CABA). The aim of our study was to characterize phylogenetically N. risticii detected in CABA. The three DNA samples previously obtained were positive by nested-PCR for a fragment of 16S rRNA of Neorickettsia genus and were sequenced, resulting in a 100% similarity among them and 99.5 to 99.7% with various strains of N. risticii from USA. Only one sample could be amplified by nested-PCR for a fragment of groESL gene. Phylogenetic analysis revealed that the current strain is related to strains of N. risticii along the west coast of the USA. Given the potential impact of veterinary medicine, molecular characterization of N. risticii circulating in South America is of great importance. In addition, the circulation of this pathogen in our country requires additional studies to understand the ecology and transmission of N. risticii, and detecting the occurrence of equine cases.\n |
| format |
Artículo Artículo publishedVersion |
| author |
Cicuttin, G.L. Greiman, S.E. |
| author_facet |
Cicuttin, G.L. Greiman, S.E. |
| author_sort |
Cicuttin, G.L. |
| title |
InVet. 2016, 18 (2): 341-347 ISSN 1514-6634 (impr ANÁLISIS FIL eso) ... |
| title_short |
InVet. 2016, 18 (2): 341-347 ISSN 1514-6634 (impr ANÁLISIS FIL eso) ... |
| title_full |
InVet. 2016, 18 (2): 341-347 ISSN 1514-6634 (impr ANÁLISIS FIL eso) ... |
| title_fullStr |
InVet. 2016, 18 (2): 341-347 ISSN 1514-6634 (impr ANÁLISIS FIL eso) ... |
| title_full_unstemmed |
InVet. 2016, 18 (2): 341-347 ISSN 1514-6634 (impr ANÁLISIS FIL eso) ... |
| title_sort |
invet. 2016, 18 (2): 341-347 issn 1514-6634 (impr análisis fil eso) ... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2016 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3477 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3477.dir/3477.PDF |
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AT cicuttingl invet2016182341347issn15146634impranalisisfileso AT greimanse invet2016182341347issn15146634impranalisisfileso AT cicuttingl analisisfilogeneticodeneorickettsiaristiciidetectadaenmurcielagosdeargentina AT greimanse analisisfilogeneticodeneorickettsiaristiciidetectadaenmurcielagosdeargentina |
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1824359749408260096 |
| spelling |
I28-R145-HWA_34772024-11-05 InVet. 2016, 18 (2): 341-347 ISSN 1514-6634 (impr ANÁLISIS FIL eso) ... Potomac horse fever (equine monocytic ehrlichiosis) is caused by Neorickettsia risticii (formerly Ehrlichia risticii), with clinical cases reported only in the USA, Brazil and Uruguay. N. risticii is an endosymbiont of flukes with a complex life cycle utilizing various hosts such as aquatic snails and arthropods, and vertebrate insectivores. Different phylogenetic studies revealed that N. risticii consists of various strains in the USA, but no information is available from Brazil and Uruguay. In our country, N. risticii was first detected in 2013 in Tadarida brasiliensis bats from Buenos Aires city\n(CABA). The aim of our study was to characterize phylogenetically N. risticii detected in CABA. The three DNA samples previously obtained were positive by nested-PCR for a fragment of 16S rRNA of Neorickettsia genus and were sequenced, resulting in a 100% similarity among them and 99.5 to 99.7% with various strains of N. risticii from USA. Only one sample could be amplified by nested-PCR for a fragment of groESL gene. Phylogenetic analysis revealed that the current strain is related to strains of N. risticii along the west coast of the USA. Given the potential impact of veterinary medicine, molecular characterization of N. risticii circulating in South America is of great importance. In addition, the circulation of this pathogen in our country requires additional studies to understand the ecology and transmission of N. risticii, and detecting the occurrence of equine cases.\n Fil: Cicuttin, G.L. Instituto de Zoonosis Luis Pasteur. Laboratorio de Zoonosis Bacterianas y Parasitarias Transmitidas por Vectores. Buenos Aires, Argentina Fil: Greiman, S.E. Georgia Southern University. Department of Biology. Georgia, USA Cicuttin, G.L. Greiman, S.E. 2016 La fiebre equina del Potomac (ehrlichiosis monocítica equina) es causada por Neorickettsia risticii (anteriormente Ehrlichia risticii), con casos clínicos notificados solamente en EEUU, Canadá, Brasil y Uruguay. Neorickettsia risticii es un endosimbionte de trematodos que presentan un complejo ciclo de vida con varios hospedadores como artrópodos, caracoles acuáticos y vertebrados insectívoros. Distintos estudios filogenéticos revelaron que se compone de diversas cepas en EEUU, pero no se cuenta con información disponible de Brasil y Uruguay. En nuestro país, N. risticii se detectó por primera vez en 2013 en murciélagos Tadarida brasiliensis procedentes de la Ciudad Autónoma de Buenos Aires (CABA). El objetivo de nuestro estudio fue caracterizar filogenéticamente a N. risticii detectada en CABA anteriormente. Se estudiaron tres muestras de ADN previamente obtenidas resultando positivas mediante una PCR anidada para un fragmento del ARNr 16S del género Neorickettsia. Las secuencias tuvieron un 100 % de similitud entre ellas y 99,5 a 99,7 % con distintos hallazgos de N. risticii en EEUU. Sólo una muestra pudo ser amplificada con la PCR anidada de un fragmento del gen groESL y el análisis filogenético reveló que N. risticii hallada en CABA se relaciona con hallazgos de la costa oeste de EEUU. Dado el potencial impacto en medicina veterinaria, la caracterización molecular de N. risticii circulante en Sudamérica es de suma importancia. Además, la circulación de este patógeno en nuestro país hace necesario realizar estudios adicionales para comprender la ecología y transmisión de N. risticii, y detectar la aparición de casos equinos.\n application/pdf 1514-6634 (impreso) 1668-3498 (en línea) Neorickettsia risticii Ehrlichiosis monocítica equina Fiebre equina del Potomac Murciélagos Argentina Neorickettsia risticii Equine monocytic ehrlichiosis Potomac horse fever Bats Argentina spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 18, nº2 Análisis filogenético de Neorickettsia risticii detectada en murciélagos de Argentina info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3477 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3477.dir/3477.PDF |