InVet. 2016, 18 (2): 323-331 ISSN 1514-6634 (impreso) BIOMARCADORES...
Mastitis is a disease with increased morbidity and mortality within dairy herds, seriously affecting milk production and milk quality. That's why in this work the activity of Metalloproteinase 2 and 9, from milk of cows and heifers with subclinical and clinical mastitis and in healthy animals w...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2016
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3475 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3475.dir/3475.PDF |
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I28-R145-HWA_3475 |
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I28-R145-HWA_34752024-11-05 InVet. 2016, 18 (2): 323-331 ISSN 1514-6634 (impreso) BIOMARCADORES... Mastitis is a disease with increased morbidity and mortality within dairy herds, seriously affecting milk production and milk quality. That's why in this work the activity of Metalloproteinase 2 and 9, from milk of cows and heifers with subclinical and clinical mastitis and in healthy animals was evaluated. Total protein fractions and different caseinolytic activity were also evaluated. The activity of Metalloproteinase 2 and 9 was assessed by zymography as caseinolytic activity and total protein fractions by SDS-PAGE. The results showed a large increase in the activity of Metalloproteinase 2 and 9 in clinical mastitis compared with subclinical and healthy animals, these results were not significant due to the large standard deviation due to the large variability among pathogens within the groups. Significant differences were found between the group Streptococcus spp. and sub-clinical and healthy groups in terms of total caseinolytic activity (p <0.05), and significant differences were observed in terms of total casein in the same groups (p <0.01). Fil: Caggiano N. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal. Buenos Aires, Argentina Fil: Bottini J.M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal. Buenos Aires, Argentina Fil: Lorenzo Smirnoff A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal. Buenos Aires, Argentina Fil: De Simone E.A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal. Buenos Aires, Argentina Fil: Chiappe Barbará M.A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal. Buenos Aires, Argentina Caggiano N. Bottini J.M. Lorenzo Smirnoff A. De Simone E.A. Chiappe Barbará M.A. 2016 La mastitis es la patología con mayor morbilidad y mortalidad dentro de los rodeos lecheros, afectando seriamente a la producción láctea y la calidad de la leche. Es por eso que en este trabajo se evaluó la actividad de las Metaloproteasas 2 y 9, involucradas en la degradación de la matriz extracelular, en leche de vacas y vaquillonas con mastitis clínica y subclínica y en animales sanos. Se evaluaron también distintas fracciones proteicas y la actividad caseinolítica total. Las fracciones proteicas se evaluaron mediante SDS PAGE y a actividad de las Metaloproteasas 2 y 9 fue evaluada mediante zimografía al igual que la actividad caseinolítica total. Los resultados arrojaron en la actividad caseinolítica total diferencias significativas entre el grupo Streptococcus spp. y los grupos subclínicas y sanas (p<0.05), y también se observaron diferencias significativas en cuanto a la caseína total entre los mismos grupos (p<0.01). Si bien las Metaloproteasas 2 y 9 en las mastitis clínicas se encontraron aumentadas las mismas no presentaron diferencias significativas. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) Mastitis Metaloproteasas Caseína Mastitis Metalloproteinases Caseine spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 18, nº2 Determinación de biomarcadores moleculares en leche y su posible aplicación en el diagnóstico y tratamiento de la mastitis bovina info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3475 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3475.dir/3475.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Mastitis Metaloproteasas Caseína Mastitis Metalloproteinases Caseine |
| spellingShingle |
Mastitis Metaloproteasas Caseína Mastitis Metalloproteinases Caseine Caggiano N. Bottini J.M. Lorenzo Smirnoff A. De Simone E.A. Chiappe Barbará M.A. InVet. 2016, 18 (2): 323-331 ISSN 1514-6634 (impreso) BIOMARCADORES... |
| topic_facet |
Mastitis Metaloproteasas Caseína Mastitis Metalloproteinases Caseine |
| description |
Mastitis is a disease with increased morbidity and mortality within dairy herds, seriously affecting milk production and milk quality. That's why in this work the activity of Metalloproteinase 2 and 9, from milk of cows and heifers with subclinical and clinical mastitis and in healthy animals was evaluated. Total protein fractions and different caseinolytic activity were also evaluated. The activity of Metalloproteinase 2 and 9 was assessed by zymography as caseinolytic activity and total protein fractions by SDS-PAGE. The results showed a large increase in the activity of Metalloproteinase 2 and 9 in clinical mastitis compared with subclinical and healthy animals, these results were not significant due to the large standard deviation due to the large variability among pathogens within the groups. Significant differences were found between the group Streptococcus spp. and sub-clinical and healthy groups in terms of total caseinolytic activity (p <0.05), and significant differences were observed in terms of total casein in the same groups (p <0.01). |
| format |
Artículo Artículo publishedVersion |
| author |
Caggiano N. Bottini J.M. Lorenzo Smirnoff A. De Simone E.A. Chiappe Barbará M.A. |
| author_facet |
Caggiano N. Bottini J.M. Lorenzo Smirnoff A. De Simone E.A. Chiappe Barbará M.A. |
| author_sort |
Caggiano N. |
| title |
InVet. 2016, 18 (2): 323-331 ISSN 1514-6634 (impreso) BIOMARCADORES... |
| title_short |
InVet. 2016, 18 (2): 323-331 ISSN 1514-6634 (impreso) BIOMARCADORES... |
| title_full |
InVet. 2016, 18 (2): 323-331 ISSN 1514-6634 (impreso) BIOMARCADORES... |
| title_fullStr |
InVet. 2016, 18 (2): 323-331 ISSN 1514-6634 (impreso) BIOMARCADORES... |
| title_full_unstemmed |
InVet. 2016, 18 (2): 323-331 ISSN 1514-6634 (impreso) BIOMARCADORES... |
| title_sort |
invet. 2016, 18 (2): 323-331 issn 1514-6634 (impreso) biomarcadores... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2016 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3475 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3475.dir/3475.PDF |
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1824359749155553280 |