InVet. 2016, 18 (2): 317-321 ISSN 1514-6634 (impr CR eso) OMOGRANINA A EN...

The aim of the present study was to identify the presence of hormonal secretory cells and localize them in the fetal and adult duodena of the coypu (Myocastor coypus bonariensis), using a polyclonal antibody developed for detection of chromogranin A. Duodena from fetus aging 60, 75, 90, 105, 120 and...

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Autores principales: Eyheramendy, V., Ortega, H., Baravalle, C., Felipe, A.
Formato: Artículo publishedVersion
Lenguaje:Español
Publicado: Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. 2016
Materias:
Duodeno
Coipo
Cromogranina A
Myocastor coypus
Feto
Duodenum
Coypu
Chromogranin A
Fetus
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3474
https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3474.dir/3474.PDF
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_3474
record_format dspace
spelling I28-R145-HWA_34742024-11-05 InVet. 2016, 18 (2): 317-321 ISSN 1514-6634 (impr CR eso) OMOGRANINA A EN... The aim of the present study was to identify the presence of hormonal secretory cells and localize them in the fetal and adult duodena of the coypu (Myocastor coypus bonariensis), using a polyclonal antibody developed for detection of chromogranin A. Duodena from fetus aging 60, 75, 90, 105, 120 and 135 days post-coitum (dpc), and from adults were used. Samples were fixed and then embedded in paraffin until analysis. Serial cuts of 5 mm were obtained and were processed with routine histological techniques. An immunohistochemical technique (avidin-biotin-peroxidase) was used to detect the hormonal secretory cells. In adults, chromogranin A was identified in cells next to the enterocytes of the surface epithelium of the intestinal villi and in the luminal epithelium of Lieberkhün crypts. In fetal duodenum of 90 dpc, positive cells were detected between the epithelial cells, while in the duodenum of fetus of 120 and 135 dpc, the positive cells were distributed heterogeneously and\nshowing morphological diversity. Fil: Eyheramendy, V. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Ciencias Morfológicas. Grupo de Investigaciones Biológicas. Tandil, Argentina Fil: Ortega, H. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Santa Fe, Argentina Fil: Ortega, H. CONICET. Santa Fe, Argentina Fil: Baravalle, C. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Santa Fe, Argentina Fil: Baravalle, C. CONICET. Santa Fe, Argentina Fil: Felipe, A. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Ciencias Morfológicas. Grupo de Investigaciones Biológicas. Tandil, Argentina Eyheramendy, V. Ortega, H. Baravalle, C. Felipe, A. 2016 El objetivo de este trabajo fue identificar la presencia y localización de células secretoras de hormonas en el duodeno del coipo (Myocastor coypus bonariensis) tanto en estadios fetales como en el adulto mediante la utilización de un anticuerpo policlonal anticromogranina A. Se trabajó con duodenos de fetos de 60, 75, 90, 105, 120 y 135 días poscoito (dpc) y de adultos.Las muestras se fijaron en formol bufferado al 10%, se procesaron con técnicas histológicas de rutina y se efectuaron cortes seriados de 5 mm. Para la determinación de células secretoras de hormonas se utilizó el método avidina-biotina-peroxidasa. En adultos, la cromogranina A fue identificada en células subyacentes a los enterocitos del epitelio de revestimiento de las vellosidades y formando parte del epitelio de revestimiento de las criptas de Lieberkhün. En los cortes de duodeno de fetos de 90 dpc se identificaron células inmunopositivas localizadas entre las células epiteliales. En los fetos de 120 y 135 dpc, las células inmunopositivas mostraron una distribución heterogénea y diversidad morfológica. application/pdf 1514-6634 (impreso) 1668-3498 (en l?nea) Duodeno Coipo Cromogranina A Myocastor coypus Feto Duodenum Coypu Chromogranin A Myocastor coypus Fetus spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 18, nº2 Presencia de cromogranina a en el duodeno fetal y adulto de Myocastor coypus bonariensis (coipo) info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3474 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3474.dir/3474.PDF
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic Duodeno
Coipo
Cromogranina A
Myocastor coypus
Feto
Duodenum
Coypu
Chromogranin A
Myocastor coypus
Fetus
spellingShingle Duodeno
Coipo
Cromogranina A
Myocastor coypus
Feto
Duodenum
Coypu
Chromogranin A
Myocastor coypus
Fetus
Eyheramendy, V.
Ortega, H.
Baravalle, C.
Felipe, A.
InVet. 2016, 18 (2): 317-321 ISSN 1514-6634 (impr CR eso) OMOGRANINA A EN...
topic_facet Duodeno
Coipo
Cromogranina A
Myocastor coypus
Feto
Duodenum
Coypu
Chromogranin A
Myocastor coypus
Fetus
description The aim of the present study was to identify the presence of hormonal secretory cells and localize them in the fetal and adult duodena of the coypu (Myocastor coypus bonariensis), using a polyclonal antibody developed for detection of chromogranin A. Duodena from fetus aging 60, 75, 90, 105, 120 and 135 days post-coitum (dpc), and from adults were used. Samples were fixed and then embedded in paraffin until analysis. Serial cuts of 5 mm were obtained and were processed with routine histological techniques. An immunohistochemical technique (avidin-biotin-peroxidase) was used to detect the hormonal secretory cells. In adults, chromogranin A was identified in cells next to the enterocytes of the surface epithelium of the intestinal villi and in the luminal epithelium of Lieberkhün crypts. In fetal duodenum of 90 dpc, positive cells were detected between the epithelial cells, while in the duodenum of fetus of 120 and 135 dpc, the positive cells were distributed heterogeneously and\nshowing morphological diversity.
format Artículo
Artículo
publishedVersion
author Eyheramendy, V.
Ortega, H.
Baravalle, C.
Felipe, A.
author_facet Eyheramendy, V.
Ortega, H.
Baravalle, C.
Felipe, A.
author_sort Eyheramendy, V.
title InVet. 2016, 18 (2): 317-321 ISSN 1514-6634 (impr CR eso) OMOGRANINA A EN...
title_short InVet. 2016, 18 (2): 317-321 ISSN 1514-6634 (impr CR eso) OMOGRANINA A EN...
title_full InVet. 2016, 18 (2): 317-321 ISSN 1514-6634 (impr CR eso) OMOGRANINA A EN...
title_fullStr InVet. 2016, 18 (2): 317-321 ISSN 1514-6634 (impr CR eso) OMOGRANINA A EN...
title_full_unstemmed InVet. 2016, 18 (2): 317-321 ISSN 1514-6634 (impr CR eso) OMOGRANINA A EN...
title_sort invet. 2016, 18 (2): 317-321 issn 1514-6634 (impr cr eso) omogranina a en...
publisher Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
publishDate 2016
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3474
https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3474.dir/3474.PDF
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