Efecto inmunomodulador del SLPI en trasplante renal
Kidney transplantation is a therapeutic procedure that has made possible to prolong life of\nindividuals with chronic renal dysfunction. However, even today graft survival is not possible\nwithout a patient under immunosuppressive treatment, modifies the homeostasis of the\nimmune system, jeopardizi...
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| Autor principal: | |
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| Formato: | Tesis de maestría acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2016
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| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_2808 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_2808.dir/2808.PDF |
| Aporte de: |
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I28-R145-HWA_2808 |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
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spa |
| topic |
SLPI Inmunología Trasplante renal Monitoreo SLPI Immunology Kidney transplant Monitoring Ciencias de la vida |
| spellingShingle |
SLPI Inmunología Trasplante renal Monitoreo SLPI Immunology Kidney transplant Monitoring Ciencias de la vida Roteta Rocamora, Julia Efecto inmunomodulador del SLPI en trasplante renal |
| topic_facet |
SLPI Inmunología Trasplante renal Monitoreo SLPI Immunology Kidney transplant Monitoring Ciencias de la vida |
| description |
Kidney transplantation is a therapeutic procedure that has made possible to prolong life of\nindividuals with chronic renal dysfunction. However, even today graft survival is not possible\nwithout a patient under immunosuppressive treatment, modifies the homeostasis of the\nimmune system, jeopardizing patient's life. That´s why strategies to evaluate patient's\nimmune status are required. The aim of this work was to study the role of an inhibitor of\nserine proteases called SLPI in renal transplant patients, with the intent to determine if this\nparameter could be useful as an indicator of immune status of the immunosuppressed\npatient. The results indicated that renal transplant patients have elevated levels of serum\nSLPI compared with healthy individuals. However, these levels were lower compared to\nthose seen in patients in pre-transplantation. Furthermore, immunosuppressive therapy\ninfluences SLPI levels as patients treated with mycophenolate mofetil + rapamycin +\nsteroids have higher levels patients treated with mycophenolate mofetil + steroids +\ntacrolimus. As expected, the proliferative capacity of mononuclear leukocytes in response to\na mitogen was lower in immunosuppressed patients compared to healthy individuals.\nHowever, treatment of cells in vitro with SLPI produced a decrease in cell proliferation\ninduced by a mitogen, in both groups of individuals (healthy and immunosuppressed).\nInterestingly, those individuals with higher plasma levels of SLPI had lower rates of cell\nproliferation, but correlated with reduced renal function. Overall, these results indicate that\nSLPI is a marker of renal damage with an immunomodulatory capacity. Its possible use as a\nmarker of immunosuppressive state must be validated with a larger number of individuals\nand likely will depend on the immunosuppressive therapy in progress by that time. |
| author2 |
Chuluyán, Eduardo |
| author_facet |
Chuluyán, Eduardo Roteta Rocamora, Julia |
| format |
Tesis de maestría Tesis de maestría acceptedVersion |
| author |
Roteta Rocamora, Julia |
| author_sort |
Roteta Rocamora, Julia |
| title |
Efecto inmunomodulador del SLPI en trasplante renal |
| title_short |
Efecto inmunomodulador del SLPI en trasplante renal |
| title_full |
Efecto inmunomodulador del SLPI en trasplante renal |
| title_fullStr |
Efecto inmunomodulador del SLPI en trasplante renal |
| title_full_unstemmed |
Efecto inmunomodulador del SLPI en trasplante renal |
| title_sort |
efecto inmunomodulador del slpi en trasplante renal |
| publisher |
Facultad de Farmacia y Bioquímica |
| publishDate |
2016 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_2808 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_2808.dir/2808.PDF |
| work_keys_str_mv |
AT rotetarocamorajulia efectoinmunomoduladordelslpientrasplanterenal |
| _version_ |
1766017514953768960 |
| spelling |
I28-R145-HWA_28082019-09-27 Kidney transplantation is a therapeutic procedure that has made possible to prolong life of\nindividuals with chronic renal dysfunction. However, even today graft survival is not possible\nwithout a patient under immunosuppressive treatment, modifies the homeostasis of the\nimmune system, jeopardizing patient's life. That´s why strategies to evaluate patient's\nimmune status are required. The aim of this work was to study the role of an inhibitor of\nserine proteases called SLPI in renal transplant patients, with the intent to determine if this\nparameter could be useful as an indicator of immune status of the immunosuppressed\npatient. The results indicated that renal transplant patients have elevated levels of serum\nSLPI compared with healthy individuals. However, these levels were lower compared to\nthose seen in patients in pre-transplantation. Furthermore, immunosuppressive therapy\ninfluences SLPI levels as patients treated with mycophenolate mofetil + rapamycin +\nsteroids have higher levels patients treated with mycophenolate mofetil + steroids +\ntacrolimus. As expected, the proliferative capacity of mononuclear leukocytes in response to\na mitogen was lower in immunosuppressed patients compared to healthy individuals.\nHowever, treatment of cells in vitro with SLPI produced a decrease in cell proliferation\ninduced by a mitogen, in both groups of individuals (healthy and immunosuppressed).\nInterestingly, those individuals with higher plasma levels of SLPI had lower rates of cell\nproliferation, but correlated with reduced renal function. Overall, these results indicate that\nSLPI is a marker of renal damage with an immunomodulatory capacity. Its possible use as a\nmarker of immunosuppressive state must be validated with a larger number of individuals\nand likely will depend on the immunosuppressive therapy in progress by that time. Fil: Roteta Rocamora, Julia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Facultad de Farmacia y Bioquímica Chuluyán, Eduardo Roteta Rocamora, Julia 2016-10-20 El trasplante renal es un procedimiento terapéutico que ha logrado prolongar la vida de los individuos con insuficiencia renal crónica. Sin embargo, aún hoy no es posible la sobrevida del injerto sin que el paciente deba ser sometido a un tratamiento inmunosupresor que altera la homeostasis del sistema inmune, poniendo en riesgo la vida del paciente. Es por esto que se requieren estrategias tendientes a evaluar el estado inmunológico del paciente. El objetivo de este trabajo fue estudiar la función de un inhibidor de serino proteasas denominado SLPI en los pacientes trasplantados renales, con la intención de determinar si este parámetro podría ser útil como indicador del estado inmunológico del paciente inmunosuprimido. Los resultados indicaron que los pacientes trasplantados renales presentan niveles elevados de SLPI sérico comparados con los individuos sanos. Sin embargo, estos niveles fueron menores comparados con los que se observan en los pacientes en el pre-trasplante. Por otro lado, el tratamiento inmunosupresor influye sobre los niveles de SLPI, ya que los pacientes tratados con micofenolato mofetil + esteroides + rapamicina presentan niveles superiores a los pacientes tratados con micofenolato mofetil + esteroides + tacrolimus. Como era de esperar, la capacidad proliferativa de los leucocitos mononucleraes en respuesta a mitógenos fue menor en los pacientes inmunosuprimidos con respecto a los individuos sanos. Sin embargo, el tratamiento de las células in vitro con SLPI, produjo una disminución de la proliferación celular inducida por un mitógeno, en ambos grupos de individuos (inmunosuprimidos y sanos). Llamativamente, aquellos individuos con mayores niveles de SLPI plasmático presentaron menores índices de proliferación celular, pero se correlacionaron con una menor función renal. En general estos resultados indican que el SLPI es un marcador de daño renal con capacidad inmunomoduladora. Su posible utilización como marcador del estado inmunosupresor deberá ser validado con un número mayor de individuos y probablemente dependerá del tratamiento inmunosupresor instaurado. application/pdf Álvarez, Élida Cantarovich, Félix Hajos, Silvia SLPI Inmunología Trasplante renal Monitoreo SLPI Immunology Kidney transplant Monitoring spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencias de la vida Efecto inmunomodulador del SLPI en trasplante renal info:eu-repo/semantics/masterThesis info:ar-repo/semantics/tesis de maestría info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_2808 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_2808.dir/2808.PDF |