Rol de las subunidades GluN2A y GluN2B, del receptor NMDA en la formación de memorias

The N-methyl D-Aspartate glutamate receptors (RNMDA) take part in processes of neuronal\nand synaptic plasticity and are believe to be involved in memory processing. They are some\nof the most important types of receptors that mediate the special transmission by glutamate,\nthough not the current ne...

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Autor principal: Ubilla Gallegos, Carmen
Otros Autores: Kornisiuk, Edgar
Formato: Tesis de maestría acceptedVersion
Lenguaje:Español
Publicado: Facultad de Farmacia y Bioquímica 2018
Materias:
NMDA
GluN2A
GluN2B
Memoria
Ciencias de la vida
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_2758
http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_2758.dir/2758.PDF
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_2758
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spelling I28-R145-HWA_27582022-02-16 The N-methyl D-Aspartate glutamate receptors (RNMDA) take part in processes of neuronal\nand synaptic plasticity and are believe to be involved in memory processing. They are some\nof the most important types of receptors that mediate the special transmission by glutamate,\nthough not the current neurotransmission, which is mediated by receptors such as RAMPA.\nThe RNMDA are expressed in the whole nervous central system, especially in the\nhippocampus, cerebral structure that takes part in the formation of special memories, giving\nplace to long term memories.\nIn this work it has been studied the role of the regulatory subunits (GluN2A and GluN2B) of\nthe RNMDA in the establishment of new memories, by reducing their expression. We used\nviral vectors that express anti-sense sequences against the ARNms of the GluN2A and\nGluN2B subunits. These vectors were inoculated into the hippocampus using estereotaxic\nsurgery in Wistar rats, and their effects in learning and memory were evaluated using Open\nField (OF) and Inhibitory Avoidance (IA) tests. The expression of the transgenes was\nevaluated by ?Western blot" and "Polimerase Chain Reaction?. We observed a significant\ndecrease of the expression of GluN2A, in the group of rates inoculated with vectors that\nexpressed sequences anti-sense against this subunit. Nevertheless, this decrease did not\naffected the performance of the animals in the behavioral tests mentioned before, showing\nworking and long term memory. Fil: Ubilla Gallegos, Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Kornisiuk, Edgar Facultad de Farmacia y Bioquímica Jerusalinsky, Diana Ubilla Gallegos, Carmen 2018-06-26 El receptor de glutamato N-metil D-Aspartato (RNMDA) participa en procesos de plasticidad\nneuronal y sináptica que se creen involucrados en el procesamiento de memorias. Es uno de\nlos principales tipos de receptores que median la transmisión especial por glutamato, aunque\nno la neurotransmisión corriente, la cual se encuentra mediada por receptores de tipo AMPA.\nEl RNMDA se expresa en todo el sistema nervioso central, en especial en el hipocampo,\nestructura cerebral que participa en la formación de memorias espaciales dando lugar a\nmemorias de largo plazo.\nEn este trabajo se estudió el rol de las subunidades regulatorias (GluN2A y GluN2B) del\nRNMDA en el establecimiento de nuevas memorias, mediante la disminución de la expresión\nde las mismas. Para ello se utilizaron vectores virales que expresan secuencias anti-sentido\ncontra los ARNms de las subunidades GluN2A y GluN2B. Estos vectores fueron inoculados\nintrahipocampo mediante cirugía estereotáxica en ratas de la cepa Wistar y se evaluó su\nefecto sobre el aprendizaje y la memoria a través de los test comportamentales de Campo\nAbierto (CA) y Evitación Inhibitoria (EI). La expresión de los transgenes se evaluó mediante\ntécnicas de biología molecular, Western blot o Polimerase Chain Reaction. Se observó una\ndisminución significativa de la expresión de GluN2A, en el grupo de ratas inoculadas con\nvectores que expresaron secuencias anti-sentido contra esta subunidad. Sin embargo, esta\ndisminución no afectó el desempeño de los animales en las pruebas comportamentales\nmencionadas, mostrando memoria de trabajo y de largo plazo. application/pdf Boccia, Mariano Delorenzi, Alejandro Bekinschtein, Pedro A. NMDA GluN2A GluN2B Memoria spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencias de la vida Rol de las subunidades GluN2A y GluN2B, del receptor NMDA en la formación de memorias info:eu-repo/semantics/masterThesis info:ar-repo/semantics/tesis de maestría info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_2758 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_2758.dir/2758.PDF
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic NMDA
GluN2A
GluN2B
Memoria
Ciencias de la vida
spellingShingle NMDA
GluN2A
GluN2B
Memoria
Ciencias de la vida
Ubilla Gallegos, Carmen
Rol de las subunidades GluN2A y GluN2B, del receptor NMDA en la formación de memorias
topic_facet NMDA
GluN2A
GluN2B
Memoria
Ciencias de la vida
description The N-methyl D-Aspartate glutamate receptors (RNMDA) take part in processes of neuronal\nand synaptic plasticity and are believe to be involved in memory processing. They are some\nof the most important types of receptors that mediate the special transmission by glutamate,\nthough not the current neurotransmission, which is mediated by receptors such as RAMPA.\nThe RNMDA are expressed in the whole nervous central system, especially in the\nhippocampus, cerebral structure that takes part in the formation of special memories, giving\nplace to long term memories.\nIn this work it has been studied the role of the regulatory subunits (GluN2A and GluN2B) of\nthe RNMDA in the establishment of new memories, by reducing their expression. We used\nviral vectors that express anti-sense sequences against the ARNms of the GluN2A and\nGluN2B subunits. These vectors were inoculated into the hippocampus using estereotaxic\nsurgery in Wistar rats, and their effects in learning and memory were evaluated using Open\nField (OF) and Inhibitory Avoidance (IA) tests. The expression of the transgenes was\nevaluated by ?Western blot" and "Polimerase Chain Reaction?. We observed a significant\ndecrease of the expression of GluN2A, in the group of rates inoculated with vectors that\nexpressed sequences anti-sense against this subunit. Nevertheless, this decrease did not\naffected the performance of the animals in the behavioral tests mentioned before, showing\nworking and long term memory.
author2 Kornisiuk, Edgar
author_facet Kornisiuk, Edgar
Ubilla Gallegos, Carmen
format Tesis de maestría
Tesis de maestría
acceptedVersion
author Ubilla Gallegos, Carmen
author_sort Ubilla Gallegos, Carmen
title Rol de las subunidades GluN2A y GluN2B, del receptor NMDA en la formación de memorias
title_short Rol de las subunidades GluN2A y GluN2B, del receptor NMDA en la formación de memorias
title_full Rol de las subunidades GluN2A y GluN2B, del receptor NMDA en la formación de memorias
title_fullStr Rol de las subunidades GluN2A y GluN2B, del receptor NMDA en la formación de memorias
title_full_unstemmed Rol de las subunidades GluN2A y GluN2B, del receptor NMDA en la formación de memorias
title_sort rol de las subunidades glun2a y glun2b, del receptor nmda en la formación de memorias
publisher Facultad de Farmacia y Bioquímica
publishDate 2018
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_2758
http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_2758.dir/2758.PDF
work_keys_str_mv AT ubillagallegoscarmen roldelassubunidadesglun2ayglun2bdelreceptornmdaenlaformaciondememorias
_version_ 1766017510073696256

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