InVet. 2015, 17 (2): 195-201 ISSN 1514-6634 (impr SUB eso) TIPOS...
Mycoplasma bovis is the most important cause of mycoplasmal mastitis in dairy cows and has been diagnosed in our country and worldwide. MLVA analysis seems to be a suitable tool to type this pathogen, for epidemiological studies of outbreaks. In the present work, 7 strains of M. bovis were analyzed....
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2015
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_1304 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_1304.dir/1304.PDF |
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I28-R145-HWA_13042024-11-05 InVet. 2015, 17 (2): 195-201 ISSN 1514-6634 (impr SUB eso) TIPOS... Mycoplasma bovis is the most important cause of mycoplasmal mastitis in dairy cows and has been diagnosed in our country and worldwide. MLVA analysis seems to be a suitable tool to type this pathogen, for epidemiological studies of outbreaks. In the present work, 7 strains of M. bovis were analyzed. They had been obtained from 2 different herds (milk samples from bulk tank milk and mammary quarters of cows with and without mastitis´s clinical signs) and had been previously identified. Four tandem repeat regions (TR) were studied. Among the TR loci studied, variability was found in only one of them (TR52), in which 2 possible alleles were observed in samples from cows with clinical mastitis. According to the author´s knowledge this is the first report of the use of MLVA for the genetic subtyping of M. bovis strains in Argentina. Fil: Estanguet, A.A. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento Patología Animal. Río Cuarto, Argentina Fil: Tamiozzo, P.J. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento Patología Animal. Río Cuarto, Argentina Fil: Tamiozzo, P.J. CONICET. Argentina Fil: Raviolo, J. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento Producción Animal. Río Cuarto, Argentina Fil: Giraudo, J. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento Patología Animal. Río Cuarto, Argentina Fil: Ambrogi, A. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento Patología Animal. Río Cuarto, Argentina Estanguet, A.A. Tamiozzo, P.J. Tirante, L. Chaves, J. Raviolo, J. Giraudo, J. Ambrogi, A. 2015 Mycoplasma bovis es el mycoplasma causante de mastitis más importante en vacas lecheras y ha sido diagnosticado en nuestro país así como a nivel mundial. Para realizar estudios epidemiológicos de brotes, el análisis por MLVA parece ser una herramienta adecuada para la tipificación genética del patógeno. En el presente trabajo se analizaron 7 cepas de M. bovis provenientes de 2 tambos distintos (muestras de leche de tanque de enfriado, y cuartos mamarios de vacas con y sin signos de mastitis) que habían sido previamente identificadas. Se estudiaron por PCR 4 regiones que comprenden secuencias repetidas en tándem (TR). Dentro de los loci TR analizados, sólo se encontró variabilidad en TR 52, en el cual se observaron 2 posibles alelos en muestras que provenían de vacas con mastitis clínica. Según el conocimiento de los autores es la primera vez que se informa la utilización del análisis MLVA para detectar subtipos genéticos entre cepas de M. bovis en Argentina. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) Ganado lechero Mastitis Mycoplasma bovis Subtipos genéticos MLVA Dairy cattle Mastitis Mycoplasma bovis Genetic subtypes MLVA spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 17, nº 2 Detección de diferentes subtipos genéticos de Mycoplasma bovis en Argentina Detection of different Mycoplasma bovis genetic subtypes in Argentina info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_1304 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_1304.dir/1304.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Ganado lechero Mastitis Mycoplasma bovis Subtipos genéticos MLVA Dairy cattle Mastitis Mycoplasma bovis Genetic subtypes MLVA |
| spellingShingle |
Ganado lechero Mastitis Mycoplasma bovis Subtipos genéticos MLVA Dairy cattle Mastitis Mycoplasma bovis Genetic subtypes MLVA Estanguet, A.A. Tamiozzo, P.J. Tirante, L. Chaves, J. Raviolo, J. Giraudo, J. Ambrogi, A. InVet. 2015, 17 (2): 195-201 ISSN 1514-6634 (impr SUB eso) TIPOS... |
| topic_facet |
Ganado lechero Mastitis Mycoplasma bovis Subtipos genéticos MLVA Dairy cattle Mastitis Mycoplasma bovis Genetic subtypes MLVA |
| description |
Mycoplasma bovis is the most important cause of mycoplasmal mastitis in dairy cows and has been diagnosed in our country and worldwide. MLVA analysis seems to be a suitable tool to type this pathogen, for epidemiological studies of outbreaks. In the present work, 7 strains of M. bovis were analyzed. They had been obtained from 2 different herds (milk samples from bulk tank milk and mammary quarters of cows with and without mastitis´s clinical signs) and had been previously identified. Four tandem repeat regions (TR) were studied. Among the TR loci studied, variability was found in only one of them (TR52), in which 2 possible alleles were observed in samples from cows with clinical mastitis. According to the author´s knowledge this is the first report of the use of MLVA for the genetic subtyping of M. bovis strains in Argentina. |
| format |
Artículo Artículo publishedVersion |
| author |
Estanguet, A.A. Tamiozzo, P.J. Tirante, L. Chaves, J. Raviolo, J. Giraudo, J. Ambrogi, A. |
| author_facet |
Estanguet, A.A. Tamiozzo, P.J. Tirante, L. Chaves, J. Raviolo, J. Giraudo, J. Ambrogi, A. |
| author_sort |
Estanguet, A.A. |
| title |
InVet. 2015, 17 (2): 195-201 ISSN 1514-6634 (impr SUB eso) TIPOS... |
| title_short |
InVet. 2015, 17 (2): 195-201 ISSN 1514-6634 (impr SUB eso) TIPOS... |
| title_full |
InVet. 2015, 17 (2): 195-201 ISSN 1514-6634 (impr SUB eso) TIPOS... |
| title_fullStr |
InVet. 2015, 17 (2): 195-201 ISSN 1514-6634 (impr SUB eso) TIPOS... |
| title_full_unstemmed |
InVet. 2015, 17 (2): 195-201 ISSN 1514-6634 (impr SUB eso) TIPOS... |
| title_sort |
invet. 2015, 17 (2): 195-201 issn 1514-6634 (impr sub eso) tipos... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias |
| publishDate |
2015 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_1304 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_1304.dir/1304.PDF |
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