Rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos

Digestive diseases caused by C. perfringens and C. difficile have a significant impact in human and veterinary medicine. In this work, no C. difficile isolates were obtained. C.perfringens was isolated from healthy and sick animals. During an outbreak of hemorrhagic enteritis C. perfringens type E (...

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Detalles Bibliográficos
Autor principal: Redondo, Leandro M.
Otros Autores: Famiglietti, Ángela
Formato: Tesis doctoral acceptedVersion
Lenguaje:Español
Publicado: Facultad de Farmacia y Bioquímica 2015
Materias:
Clostridium
C. perfringens
C. difficile
Iota
Ciencia de la vida
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1143
http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1143.dir/1143.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_1143
record_format dspace
spelling I28-R145-HWA_11432019-09-25 Digestive diseases caused by C. perfringens and C. difficile have a significant impact in human and veterinary medicine. In this work, no C. difficile isolates were obtained. C.perfringens was isolated from healthy and sick animals. During an outbreak of hemorrhagic enteritis C. perfringens type E (binary toxin producer) was isolated. This is the first report of type E illness in Argentina. Analyzed type E isolates belong to the same clonal complex, regardless of their geographic origin. This organism is highly adapted and pathogenicity is due to the combination of plasmid borne and chromosome encoded factors. In vivo it was possible to reproduce the lethal effect and determining an enterotoxigenic effect of iota toxin. Inhibition of gastrointestinal motility and changes in fluid homeostasis were observed, also it was possible reproduction of intestinal lesions. In vitro it was observed that iota toxin induces the formation of extensions on cell surface to which C. perfringens type E cells can attach differentially. Intraspecific inhibition is involved in intestinal colonization by C.perfringens type E.Type E bacteria produces an antimicrobial peptide with activity against different strains of C. perfringens. Fil: Redondo, Leandro M. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Famiglietti, Ángela Facultad de Farmacia y Bioquímica Fernández-Miyakawa, Mariano E. Redondo, Leandro M. 2015-03-27 Las enfermedades digestivas causadas por C. perfringens y C. difficile poseen un importante impacto en medicina humana y veterinaria. En este trabajo no se obtuvieron aislamientos de C. difficile. Se obtuvieron aislamientos de C. perfringens de animales sanos y enfermos. Durante un brote de enteritis hemorrágica se aisló C. perfringens tipo E, productor de toxina iota (toxina binaria). Este es el primer reporte de enfermedad por este toxinotipo en Argentina. Los aislamientos tipo E analizados pertenecen a un mismo complejo clonal, independientemente de su origen geográfico. Este microorganismo se encuentra altamente adaptado y la capacidad patógena depende de la acción combinada de factores plasmidico y cromosómicos. In vivo fue posible reproducir el efecto letal y determinar un efecto enterotoxico de la toxina iota. Se observó una inhibición de la motilidad gastrointestinal, cambios en la homeostasis de líquidos y lesiones intestinales.\nIn vitro se observó que la toxina iota induce la formación de extensiones en la superficie celular a las cuales se adhiere C. perfringens tipo E de manera diferencial. La inhibición intraespecifica contribuye en la colonización intestinal por C. perfringens tipo E. Estas cepas producen un péptido con acción antimicrobiana contra diversas cepas de C.perfringens. application/pdf Gironacci, Mariela Ibarra, Cristina Fink, Nilda Clostridium C. perfringens C. difficile Iota spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencia de la vida Rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1143 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1143.dir/1143.PDF
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic Clostridium
C. perfringens
C. difficile
Iota
Ciencia de la vida
spellingShingle Clostridium
C. perfringens
C. difficile
Iota
Ciencia de la vida
Redondo, Leandro M.
Rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos
topic_facet Clostridium
C. perfringens
C. difficile
Iota
Ciencia de la vida
description Digestive diseases caused by C. perfringens and C. difficile have a significant impact in human and veterinary medicine. In this work, no C. difficile isolates were obtained. C.perfringens was isolated from healthy and sick animals. During an outbreak of hemorrhagic enteritis C. perfringens type E (binary toxin producer) was isolated. This is the first report of type E illness in Argentina. Analyzed type E isolates belong to the same clonal complex, regardless of their geographic origin. This organism is highly adapted and pathogenicity is due to the combination of plasmid borne and chromosome encoded factors. In vivo it was possible to reproduce the lethal effect and determining an enterotoxigenic effect of iota toxin. Inhibition of gastrointestinal motility and changes in fluid homeostasis were observed, also it was possible reproduction of intestinal lesions. In vitro it was observed that iota toxin induces the formation of extensions on cell surface to which C. perfringens type E cells can attach differentially. Intraspecific inhibition is involved in intestinal colonization by C.perfringens type E.Type E bacteria produces an antimicrobial peptide with activity against different strains of C. perfringens.
author2 Famiglietti, Ángela
author_facet Famiglietti, Ángela
Redondo, Leandro M.
format Tesis doctoral
Tesis doctoral
acceptedVersion
author Redondo, Leandro M.
author_sort Redondo, Leandro M.
title Rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos
title_short Rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos
title_full Rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos
title_fullStr Rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos
title_full_unstemmed Rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos
title_sort rol de las toxinas binarias en las enfermedades intestinales causadas por clostridios enterotóxicos
publisher Facultad de Farmacia y Bioquímica
publishDate 2015
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1143
http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1143.dir/1143.PDF
work_keys_str_mv AT redondoleandrom roldelastoxinasbinariasenlasenfermedadesintestinalescausadasporclostridiosenterotoxicos
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