Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city
The aim of this work was to confirm the presence of Ehrlichia canis in blood samples of dogs from Concordia, Entre Ríos Province, Argentina. DNA was extracted in 14 blood samples of dogs with presumptive diagnosis of CME. A PCR protocol targeting dsb gene, specific for genus Ehrlichia was carried ou...
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| Autores principales: | , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Litoral
2020
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| Acceso en línea: | https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/8935 |
| Aporte de: |
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I26-R133-article-8935 |
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ojs |
| institution |
Universidad Nacional del Litoral |
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I-26 |
| repository_str |
R-133 |
| container_title_str |
Biblioteca Virtual - Publicaciones (UNL) |
| language |
Español |
| format |
Artículo revista |
| topic |
Ehrlichia canis Canine Granulocytic Ehrlichiosis Rhiphicephalus sanguineus Trombocytopenia Argentina Ehrlichia canis Ehrlichiosis Granulocítica Canina Rhipicephalus sanguineus Trombocitopenia Argentina |
| spellingShingle |
Ehrlichia canis Canine Granulocytic Ehrlichiosis Rhiphicephalus sanguineus Trombocytopenia Argentina Ehrlichia canis Ehrlichiosis Granulocítica Canina Rhipicephalus sanguineus Trombocitopenia Argentina SÁNCHEZ, Ricardo Oscar BAZZANO, Valentín FELIX, María Laura ARMÚA-FERNÁNDEZ, María Teresa VENZAL, José Manuel Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city |
| topic_facet |
Ehrlichia canis Canine Granulocytic Ehrlichiosis Rhiphicephalus sanguineus Trombocytopenia Argentina Ehrlichia canis Ehrlichiosis Granulocítica Canina Rhipicephalus sanguineus Trombocitopenia Argentina |
| author |
SÁNCHEZ, Ricardo Oscar BAZZANO, Valentín FELIX, María Laura ARMÚA-FERNÁNDEZ, María Teresa VENZAL, José Manuel |
| author_facet |
SÁNCHEZ, Ricardo Oscar BAZZANO, Valentín FELIX, María Laura ARMÚA-FERNÁNDEZ, María Teresa VENZAL, José Manuel |
| author_sort |
SÁNCHEZ, Ricardo Oscar |
| title |
Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city |
| title_short |
Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city |
| title_full |
Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city |
| title_fullStr |
Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city |
| title_full_unstemmed |
Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city |
| title_sort |
canine monocytic ehrlichiosis in entre ríos province, argentina: molecular confirmation of cases in concordia city |
| description |
The aim of this work was to confirm the presence of Ehrlichia canis in blood samples of dogs from Concordia, Entre Ríos Province, Argentina. DNA was extracted in 14 blood samples of dogs with presumptive diagnosis of CME. A PCR protocol targeting dsb gene, specific for genus Ehrlichia was carried out for detection and species confirmation was carried out by sequencing. Twelve out of 14 samples shown bands of the expected size. The obtained sequences revealed 98-100% identity with E. canis sequences registered in GenBank. Moreover, ticks were retrieved from an E. canis positive dog and a fragment of mitochondrial ARNr 16S gene was amplified. The obtained sequences were identified as R. sanguineus sensu stricto (temperate linage). This work confirmed the presence of E. canis in Concordia city, Entre Ríos province resulting in an alert for veterinary clinicians in this region aiming to encourage prevention strategies of this disease and vector control. |
| publisher |
Universidad Nacional del Litoral |
| publishDate |
2020 |
| url |
https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/8935 |
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2023-07-05T23:07:03Z |
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2024-08-13T20:38:35Z |
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1807306211126673408 |
| spelling |
I26-R133-article-89352023-09-15T10:33:35Z Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city Ehrlichiosis monocítica canina en la provincia de Entre Ríos, Argentina: confirmación molecular de casos en la ciudad de Concordia Diagnóstico parasitológico e molecular de Ehrlichia canis em cães da cidade de Concordia, Entre Ríos, Argentina SÁNCHEZ, Ricardo Oscar BAZZANO, Valentín FELIX, María Laura ARMÚA-FERNÁNDEZ, María Teresa VENZAL, José Manuel Ehrlichia canis Canine Granulocytic Ehrlichiosis Rhiphicephalus sanguineus Trombocytopenia Argentina Ehrlichia canis Ehrlichiosis Granulocítica Canina Rhipicephalus sanguineus Trombocitopenia Argentina The aim of this work was to confirm the presence of Ehrlichia canis in blood samples of dogs from Concordia, Entre Ríos Province, Argentina. DNA was extracted in 14 blood samples of dogs with presumptive diagnosis of CME. A PCR protocol targeting dsb gene, specific for genus Ehrlichia was carried out for detection and species confirmation was carried out by sequencing. Twelve out of 14 samples shown bands of the expected size. The obtained sequences revealed 98-100% identity with E. canis sequences registered in GenBank. Moreover, ticks were retrieved from an E. canis positive dog and a fragment of mitochondrial ARNr 16S gene was amplified. The obtained sequences were identified as R. sanguineus sensu stricto (temperate linage). This work confirmed the presence of E. canis in Concordia city, Entre Ríos province resulting in an alert for veterinary clinicians in this region aiming to encourage prevention strategies of this disease and vector control. El objetivo del siguiente trabajo fue confirmar la presencia de Ehrlichia canis por diagnóstico molecular, a partir de muestras de perros con diagnósticos presuntivos, oriundos de la ciudad de Concordia, provincia de Entre Ríos. ADN fue extraído de 14 muestras de sangre de perros con diagnóstico presuntivo de EMC. Para la detección de ADN de E. canis se amplificó un fragmento del gen dsb, específico de este género, y su confirmación específica se realizó mediante secuenciación. Doce de las 14 muestras mostraron bandas del tamaño esperado. Las secuencias obtenidas mostraron un 98-100% de identidad con secuencias registradas en GenBank para E. canis. Además, se amplificó un fragmento del gen ARNr 16S mitocondrial de garrapatas obtenidas de un perro positivo. Las secuencias demostraron que corresponden a R. sanguineus sensu stricto (linaje templado). En este estudio se confirma la presencia de E. canis en la ciudad de Concordia, Entre Ríos, resultando en una alerta para los médicos veterinarios de la región, a fin de incentivar estrategias de prevención de la enfermedad y control del vector. O objetivo do trabalho a seguir foi confirmar a presença de Ehrlichia canis por diagnóstico molecular, a partir de amostras de cães com diagnóstico presuntivo, originários da cidade de Concordia, província de Entre Ríos. O DNA foi extraído de 14 amostras de sangue de cães com diagnóstico presuntivo de CEM. Para a detecção do DNA de E. canis, um fragmento do gene dsb, específico para esse gênero, foi amplificado e sua confirmação específica foi realizada por sequenciamento. Doze das 14 amostras apresentaram bandas do tamanho esperado. As seqüências obtidas mostraram 98-100% de identidade com as seqüências registradas no GenBank para E. canis. Além disso, um fragmento do gene 16S rRNA mitocondrial do carrapato obtido de um cão positivo foi amplificado. As seqüências mostraram que correspondem a R. sanguineus sensu stricto (linhagem temperada). Este estudo confirma a presença de E. canis na cidade de Concórdia, Entre Ríos, resultando em um alerta para médicos veterinários da região, a fim de incentivar estratégias para prevenção de doenças e controle de vetores. Universidad Nacional del Litoral 2020-05-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original Original article application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/8935 10.14409/favecv.v19i1.8935 FAVE Sección Ciencias Veterinarias; Vol. 19 Núm. 1 (2020): FAVE Sección Ciencias Veterinarias; 16-22 FAVE Sección Ciencias Veterinarias; Vol. 19 No. 1 (2020): FAVE Sección Ciencias Veterinarias; 16-22 FAVE Sección Ciencias Veterinarias; v. 19 n. 1 (2020): FAVE Sección Ciencias Veterinarias; 16-22 2362-5589 1666-938X 10.14409/favecv.v19i1 spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/8935/12458 Derechos de autor 2020 Ricardo Oscar SÁNCHEZ, Valentín BAZZANO, María Laura FELIX, María Teresita ARMÚA-FERNÁNDEZ, José Manuel VENZAL |