Adjustment of the topographi c viability test by tetrazolium in Poncirus trifo- liata (L.) Raf.

Poncirus trifoliata (L.) Raf., one of the main rootstocks in Argentina’s citrus production, is propagated from seeds, which exhibit delayed germination due to physical dormancy. The biochemical tetrazolium test (TZ) allows a quick assessment of seed physiological quality. Even though the test has be...

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Autores principales: Catraro, M. A., Poggi, D. R., Quadrelli, A. R., Gariglio, N. F., Flores, P. C.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Litoral 2022
Materias:
seed physiological quality
hardy orange
topographic test
viability
calidad fi siológica de semillas
trifolio
test topográfico
viabilidad
Acceso en línea:https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/12005
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Biblioteca Virtual - Publicaciones (UNL) de Universidad Nacional del Litoral
id I26-R133-article-12005
record_format ojs
spelling I26-R133-article-120052024-07-31T22:25:20Z Adjustment of the topographi c viability test by tetrazolium in Poncirus trifo- liata (L.) Raf. Ajuste de la prueba de viabilidad topográfica por tetrazolio en Poncirus trifoliata (L.) Raf. Catraro, M. A. Poggi, D. R. Quadrelli, A. R. Gariglio, N. F. Flores, P. C. seed physiological quality hardy orange topographic test viability calidad fi siológica de semillas trifolio test topográfico viabilidad Poncirus trifoliata (L.) Raf., one of the main rootstocks in Argentina’s citrus production, is propagated from seeds, which exhibit delayed germination due to physical dormancy. The biochemical tetrazolium test (TZ) allows a quick assessment of seed physiological quality. Even though the test has been applied to Poncirus, specific protocols for this species have not been developed yet. In the present work, a preliminary assay was carried out to test the TZ concentration and incubation time recommended by seed analyst associations (1% for 18 and 24h), the results of which were used to establish treatments suited to Poncirus. The TZ salt concentrations evaluated were 0.2 and 0.5%, with 3 and 5 h exposure to TZ, in a 2x2x2 factorial design. Seeds were classified as viable or non-viable according to the different staining obtained. It was determined that 5 h of incubation in 0.2% TZ salt concentration of unscarified seeds is the most suitable combination for assessing viability of Poncirus trifoliata seeds. way. Poncirus trifoliata (L.) Raf., es uno de los portainjertos más importantes para la producción citrícola de la Argentina, y se propaga por semillas cuya latencia física dificulta su germinación. La prueba bioquímica de tetrazolio (TZ) permite una evaluación rápida de la calidad fisiológica de las semillas. A pesar de que la prueba se ha aplicado a Poncirus, aún no se han desarrollado protocolos específicos para esta especie. En el presente trabajo, se llevó a cabo un ensayo preliminar para pro-bar la concentración de TZ y el tiempo de incubación recomendado por las asociaciones de analistas de semillas (1% durante 18 y 24 horas), cuyos resultados se utilizaron para establecer tratamientos adecuados para la especie. Las concentraciones de sal de TZ evaluadas fueron de 0,2 y 0,5%, con una exposición de 3 y 5 horas a TZ, en un diseño factorial 2x2x2. Las semillas se clasificaron como viables o no viables de acuerdo con las diferentes tinciones obtenidas. Se determinó como la metodología más adecuada para realizar la prueba de TZ la utilización de una concentración del 0,2% de la sal TZ, 5 h de incubación y semillas sin escarificar de modo de optimizar los recursos implicados.   Universidad Nacional del Litoral 2022-08-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/zip text/html https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/12005 10.14409/fa.v21i2.12005 FAVE Sección Ciencias Agrarias; Vol. 21 Núm. 2 (2022): FAVE Sección Ciencias Agrarias; 57-66 FAVE Sección Ciencias Agrarias; Vol. 21 No. 2 (2022): FAVE Sección Ciencias Agrarias; 57-66 FAVE Sección Ciencias Agrarias; v. 21 n. 2 (2022): FAVE Sección Ciencias Agrarias; 57-66 2346-9129 1666-7719 10.14409/fa.v21i2 spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/12005/16373 https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/12005/17002 https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/12005/16374 Derechos de autor 2022 FAVE Sección Ciencias Agrarias
institution Universidad Nacional del Litoral
institution_str I-26
repository_str R-133
container_title_str Biblioteca Virtual - Publicaciones (UNL)
language Español
format Artículo revista
topic seed physiological quality
hardy orange
topographic test
viability
calidad fi siológica de semillas
trifolio
test topográfico
viabilidad
spellingShingle seed physiological quality
hardy orange
topographic test
viability
calidad fi siológica de semillas
trifolio
test topográfico
viabilidad
Catraro, M. A.
Poggi, D. R.
Quadrelli, A. R.
Gariglio, N. F.
Flores, P. C.
Adjustment of the topographi c viability test by tetrazolium in Poncirus trifo- liata (L.) Raf.
topic_facet seed physiological quality
hardy orange
topographic test
viability
calidad fi siológica de semillas
trifolio
test topográfico
viabilidad
author Catraro, M. A.
Poggi, D. R.
Quadrelli, A. R.
Gariglio, N. F.
Flores, P. C.
author_facet Catraro, M. A.
Poggi, D. R.
Quadrelli, A. R.
Gariglio, N. F.
Flores, P. C.
author_sort Catraro, M. A.
title Adjustment of the topographi c viability test by tetrazolium in Poncirus trifo- liata (L.) Raf.
title_short Adjustment of the topographi c viability test by tetrazolium in Poncirus trifo- liata (L.) Raf.
title_full Adjustment of the topographi c viability test by tetrazolium in Poncirus trifo- liata (L.) Raf.
title_fullStr Adjustment of the topographi c viability test by tetrazolium in Poncirus trifo- liata (L.) Raf.
title_full_unstemmed Adjustment of the topographi c viability test by tetrazolium in Poncirus trifo- liata (L.) Raf.
title_sort adjustment of the topographi c viability test by tetrazolium in poncirus trifo- liata (l.) raf.
description Poncirus trifoliata (L.) Raf., one of the main rootstocks in Argentina’s citrus production, is propagated from seeds, which exhibit delayed germination due to physical dormancy. The biochemical tetrazolium test (TZ) allows a quick assessment of seed physiological quality. Even though the test has been applied to Poncirus, specific protocols for this species have not been developed yet. In the present work, a preliminary assay was carried out to test the TZ concentration and incubation time recommended by seed analyst associations (1% for 18 and 24h), the results of which were used to establish treatments suited to Poncirus. The TZ salt concentrations evaluated were 0.2 and 0.5%, with 3 and 5 h exposure to TZ, in a 2x2x2 factorial design. Seeds were classified as viable or non-viable according to the different staining obtained. It was determined that 5 h of incubation in 0.2% TZ salt concentration of unscarified seeds is the most suitable combination for assessing viability of Poncirus trifoliata seeds. way.
publisher Universidad Nacional del Litoral
publishDate 2022
url https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/12005
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first_indexed 2022-10-05T02:23:54Z
last_indexed 2024-08-13T20:36:28Z
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