Development of a murine colonization model of Lactobacillus plantarum LP5 of porcine origin.

The objective of this work was to generate an experimental model of colonization by L. plantarum LP5 in mice (Mus musculus) aimed at improving the general state of the animal and preventing the possible presence of zoonotic pathogens. The mice used were females of the Balb/cCmedc strain, 6 weeks old...

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Autor principal: Ruiz, M J
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Litoral 2021
Materias:
Murine model, Colonization, Lactobacillus plantarum, Lactic acid bacteria.
Modelo murino, Colonización, Lactobacillus plantarum, Bacteria ácido láctica.
Acceso en línea:https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10938
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Biblioteca Virtual - Publicaciones (UNL) de Universidad Nacional del Litoral
id I26-R133-article-10938
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spelling I26-R133-article-109382023-09-15T10:33:20Z Development of a murine colonization model of Lactobacillus plantarum LP5 of porcine origin. DESARROLLO DE MODELO MURINO DE COLONIZACIÓN DE Lactobacillus plantarum LP5 DE ORIGEN PORCINO Ruiz, M J Murine model, Colonization, Lactobacillus plantarum, Lactic acid bacteria. Modelo murino, Colonización, Lactobacillus plantarum, Bacteria ácido láctica. The objective of this work was to generate an experimental model of colonization by L. plantarum LP5 in mice (Mus musculus) aimed at improving the general state of the animal and preventing the possible presence of zoonotic pathogens. The mice used were females of the Balb/cCmedc strain, 6 weeks old. Animals were organized into 2 groups of 3 individuals: treated (GT) and control (GC). GT was administered twice weekly by gavage with 100 µl of rifampicin resistant porcine L. plantarum LP5 at a concentration of 1010 CFU. Mice were fed water and food ad libitum throughout the experiment. Colonization in both groups was evaluated by the presence of clinical signs and by a microbiological analysis of the feces. Sampling was performed at weekly intervals to quantify the populations of the supplied L. plantarum LP5, Lactobacillus spp., Escherichia coli, total coliforms, Campylobacter and yeast. The results of the sampling in the GT in the week showed a recovery of L. plantarum LP5 above 5 logUFC/g, in the second it was 7 logUFC/g and in the third it was 5 logUFC/g. The CG, meanwhile, maintained the null count throughout the experiment. The results showed that there was no imbalance between the microbial components. Therefore, it is feasible to affirm that the inoculum did not alter the balance of the intestinal ecosystem. Particularly, it did not exert an inhibitory effect on the members of the previously established microbiota. Universidad Nacional del Litoral 2021-12-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10938 10.14409/favecv.v19isuplemento.10938 FAVE Sección Ciencias Veterinarias; Vol. 19 Núm. suplemento (2021): FAVE Sección Ciencias Veterinarias FAVE Sección Ciencias Veterinarias; Vol. 19 No. suplemento (2021): FAVE Sección Ciencias Veterinarias FAVE Sección Ciencias Veterinarias; v. 19 n. suplemento (2021): FAVE Sección Ciencias Veterinarias 2362-5589 1666-938X 10.14409/favecv.v19isuplemento spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10938/14957 Derechos de autor 2021 M J Ruiz
institution Universidad Nacional del Litoral
institution_str I-26
repository_str R-133
container_title_str Biblioteca Virtual - Publicaciones (UNL)
language Español
format Artículo revista
topic Murine model, Colonization, Lactobacillus plantarum, Lactic acid bacteria.
Modelo murino, Colonización, Lactobacillus plantarum, Bacteria ácido láctica.
spellingShingle Murine model, Colonization, Lactobacillus plantarum, Lactic acid bacteria.
Modelo murino, Colonización, Lactobacillus plantarum, Bacteria ácido láctica.
Ruiz, M J
Development of a murine colonization model of Lactobacillus plantarum LP5 of porcine origin.
topic_facet Murine model, Colonization, Lactobacillus plantarum, Lactic acid bacteria.
Modelo murino, Colonización, Lactobacillus plantarum, Bacteria ácido láctica.
author Ruiz, M J
author_facet Ruiz, M J
author_sort Ruiz, M J
title Development of a murine colonization model of Lactobacillus plantarum LP5 of porcine origin.
title_short Development of a murine colonization model of Lactobacillus plantarum LP5 of porcine origin.
title_full Development of a murine colonization model of Lactobacillus plantarum LP5 of porcine origin.
title_fullStr Development of a murine colonization model of Lactobacillus plantarum LP5 of porcine origin.
title_full_unstemmed Development of a murine colonization model of Lactobacillus plantarum LP5 of porcine origin.
title_sort development of a murine colonization model of lactobacillus plantarum lp5 of porcine origin.
description The objective of this work was to generate an experimental model of colonization by L. plantarum LP5 in mice (Mus musculus) aimed at improving the general state of the animal and preventing the possible presence of zoonotic pathogens. The mice used were females of the Balb/cCmedc strain, 6 weeks old. Animals were organized into 2 groups of 3 individuals: treated (GT) and control (GC). GT was administered twice weekly by gavage with 100 µl of rifampicin resistant porcine L. plantarum LP5 at a concentration of 1010 CFU. Mice were fed water and food ad libitum throughout the experiment. Colonization in both groups was evaluated by the presence of clinical signs and by a microbiological analysis of the feces. Sampling was performed at weekly intervals to quantify the populations of the supplied L. plantarum LP5, Lactobacillus spp., Escherichia coli, total coliforms, Campylobacter and yeast. The results of the sampling in the GT in the week showed a recovery of L. plantarum LP5 above 5 logUFC/g, in the second it was 7 logUFC/g and in the third it was 5 logUFC/g. The CG, meanwhile, maintained the null count throughout the experiment. The results showed that there was no imbalance between the microbial components. Therefore, it is feasible to affirm that the inoculum did not alter the balance of the intestinal ecosystem. Particularly, it did not exert an inhibitory effect on the members of the previously established microbiota.
publisher Universidad Nacional del Litoral
publishDate 2021
url https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10938
work_keys_str_mv AT ruizmj developmentofamurinecolonizationmodeloflactobacillusplantarumlp5ofporcineorigin
AT ruizmj desarrollodemodelomurinodecolonizaciondelactobacillusplantarumlp5deorigenporcino
first_indexed 2023-07-05T23:06:08Z
last_indexed 2024-08-13T20:37:35Z
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