Proposal of a methodology to quantify the antibacterial activity of honey.
In this study a standardized method for objectively comparing the antibacterial activity of honey was developed. The assay was performed using a strain of S. aureus and four honeys that have been shown antibacterial activity against this strain. The antibacterial activity of honeys were checked by u...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Litoral
2021
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| Acceso en línea: | https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10934 |
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I26-R133-article-10934 |
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I26-R133-article-109342023-09-15T10:33:20Z Proposal of a methodology to quantify the antibacterial activity of honey. PROPUESTA DE UNA METODOLOGÍA PARA CUANTIFICAR LA ACTIVIDAD ANTIBACTERIANA DE LA MIEL Ferrer, A Honey, Staphylococcus aureus, Quantitation, Antibacterial activity. Miel, Staphylococcus aureus, Cuantificación, Actividad antibacteriana. In this study a standardized method for objectively comparing the antibacterial activity of honey was developed. The assay was performed using a strain of S. aureus and four honeys that have been shown antibacterial activity against this strain. The antibacterial activity of honeys were checked by using an agar plate diffusion method using S. aureus (ATCC 29213) as the test organism. On the surface of each agar plate six stainless steel cylinders with a diameter of 8,14 mm were radially disposed. Onto each cylinder 0,1 mL of sample honey at 10% v/v was placed and all Petri dishes were incubated at 35°C during 24 h. Each sample were analyzed by triplicate. The surface of the cylinder diameter was set as reference antibacterial activity equivalent to minimal inhibitory concentration (MIC). The antibacterial activity of each honey was quantitated by the relationship between the surface of the inhibitory zone and the surface of the reference activity expressed as multiples of MIC (x MIC). The method allowed establishing differences between the bacterial activities of the different honeys tested and could be used for identifying honeys that could be employed with medicinal purpose for the treatments of wounds. Universidad Nacional del Litoral 2021-12-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10934 10.14409/favecv.v19isuplemento.10934 FAVE Sección Ciencias Veterinarias; Vol. 19 Núm. suplemento (2021): FAVE Sección Ciencias Veterinarias FAVE Sección Ciencias Veterinarias; Vol. 19 No. suplemento (2021): FAVE Sección Ciencias Veterinarias FAVE Sección Ciencias Veterinarias; v. 19 n. suplemento (2021): FAVE Sección Ciencias Veterinarias 2362-5589 1666-938X 10.14409/favecv.v19isuplemento spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10934/14952 Derechos de autor 2021 A Ferrer |
| institution |
Universidad Nacional del Litoral |
| institution_str |
I-26 |
| repository_str |
R-133 |
| container_title_str |
Biblioteca Virtual - Publicaciones (UNL) |
| language |
Español |
| format |
Artículo revista |
| topic |
Honey, Staphylococcus aureus, Quantitation, Antibacterial activity. Miel, Staphylococcus aureus, Cuantificación, Actividad antibacteriana. |
| spellingShingle |
Honey, Staphylococcus aureus, Quantitation, Antibacterial activity. Miel, Staphylococcus aureus, Cuantificación, Actividad antibacteriana. Ferrer, A Proposal of a methodology to quantify the antibacterial activity of honey. |
| topic_facet |
Honey, Staphylococcus aureus, Quantitation, Antibacterial activity. Miel, Staphylococcus aureus, Cuantificación, Actividad antibacteriana. |
| author |
Ferrer, A |
| author_facet |
Ferrer, A |
| author_sort |
Ferrer, A |
| title |
Proposal of a methodology to quantify the antibacterial activity of honey. |
| title_short |
Proposal of a methodology to quantify the antibacterial activity of honey. |
| title_full |
Proposal of a methodology to quantify the antibacterial activity of honey. |
| title_fullStr |
Proposal of a methodology to quantify the antibacterial activity of honey. |
| title_full_unstemmed |
Proposal of a methodology to quantify the antibacterial activity of honey. |
| title_sort |
proposal of a methodology to quantify the antibacterial activity of honey. |
| description |
In this study a standardized method for objectively comparing the antibacterial activity of honey was developed. The assay was performed using a strain of S. aureus and four honeys that have been shown antibacterial activity against this strain. The antibacterial activity of honeys were checked by using an agar plate diffusion method using S. aureus (ATCC 29213) as the test organism. On the surface of each agar plate six stainless steel cylinders with a diameter of 8,14 mm were radially disposed. Onto each cylinder 0,1 mL of sample honey at 10% v/v was placed and all Petri dishes were incubated at 35°C during 24 h. Each sample were analyzed by triplicate. The surface of the cylinder diameter was set as reference antibacterial activity equivalent to minimal inhibitory concentration (MIC). The antibacterial activity of each honey was quantitated by the relationship between the surface of the inhibitory zone and the surface of the reference activity expressed as multiples of MIC (x MIC). The method allowed establishing differences between the bacterial activities of the different honeys tested and could be used for identifying honeys that could be employed with medicinal purpose for the treatments of wounds. |
| publisher |
Universidad Nacional del Litoral |
| publishDate |
2021 |
| url |
https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10934 |
| work_keys_str_mv |
AT ferrera proposalofamethodologytoquantifytheantibacterialactivityofhoney AT ferrera propuestadeunametodologiaparacuantificarlaactividadantibacterianadelamiel |
| first_indexed |
2023-07-05T23:06:07Z |
| last_indexed |
2024-08-13T20:37:34Z |
| _version_ |
1807306147250569216 |