Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives

Vishniacozyma victoriae and Pichia membranifaciens were selected in a previous work for their biocontrol effec- tiveness against Penicillium expansum and Botrytis cinerea, the causal agents of blue and grey mold of pear fruits. The objective of this study was to determine the effects of different...

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Autores principales: Gramisci, Betina Rocío, Lutz, María Cecilia, Lopes, Christian Ariel, Sangorrin, Marcela Paula
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: Elsevier 2018
Materias:
Postharvest diseases
Antagonist yeast
Biocontrol
Nutritional profile
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
Acceso en línea:https://rdi.uncoma.edu.ar/handle/uncomaid/19296
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-19296
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spelling I22-R178-uncomaid-192962026-04-24T17:14:56Z Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives Gramisci, Betina Rocío Lutz, María Cecilia Lopes, Christian Ariel Sangorrin, Marcela Paula Postharvest diseases Antagonist yeast Biocontrol Nutritional profile Ciencias de la Tierra y Medio Ambiente Ciencias Biomédicas Artículos Vishniacozyma victoriae and Pichia membranifaciens were selected in a previous work for their biocontrol effec- tiveness against Penicillium expansum and Botrytis cinerea, the causal agents of blue and grey mold of pear fruits. The objective of this study was to determine the effects of different nutrient or additives on the growth of the two antagonist yeasts and of the two fungal pathogens in order to develop a rational selection based on nutritional profiles to be used in biocontrol enhancement of decay in pear fruits. Twenty-six different nutrient sources including 18 amino acids, five sugars, three inorganic nitrogen and one iron source were tested in vitro for their effect on the growth of the two yeasts and the two pathogens. Nutrients that promoted the growth of the yeasts and inhibited the growth of pathogens were applied with the antagonist to wounded fruits to evaluate their effect on enhancing biocontrol in cold storage. In general, the effect of each additive was specific to the yeasts and pathogens used in the in situ assays. The combination of the yeast and some additives resulted in a sig- nificantly higher activity with respect to the single treatments applied separately, producing synergistic effects. The effect of the exogenous application of CaCl2 and chitosan together with the antagonist yeasts was also evaluated against the two pathogens. The most effective mixtures were the CaCl2 with the two antagonist yeasts. Our research demonstrates that manipulating the chemical environment by adding several amino acid and/or Cl2Ca results in improved antagonistic activity of Vishniacozyma victoriae and Pichia membranifaciens against two postharvest diseases of pear fruits. Fil: Gramisci, Betina Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Gramisci, Betina Rocío. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Fil: Lutz, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Lutz, María Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Lopes, Christian Ariel. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Fil: Lopes, Christian Ariel. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Sangorrin, Marcela Paula. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. 2018 2026-04-09T17:37:04Z 2026-04-09T17:37:04Z Articulo article acceptedVersion 1049-9644 https://rdi.uncoma.edu.ar/handle/uncomaid/19296 eng https://doi.org/10.1016/j.biocontrol.2018.03.001 https://ri.conicet.gov.ar/handle/11336/89478 Atribución-NoComercial-CompartirIgual 4.0 https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf pp. 151-158 application/pdf Elsevier Biological Control 121 (2018)
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Postharvest diseases
Antagonist yeast
Biocontrol
Nutritional profile
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
spellingShingle Postharvest diseases
Antagonist yeast
Biocontrol
Nutritional profile
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
Gramisci, Betina Rocío
Lutz, María Cecilia
Lopes, Christian Ariel
Sangorrin, Marcela Paula
Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
topic_facet Postharvest diseases
Antagonist yeast
Biocontrol
Nutritional profile
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
description Vishniacozyma victoriae and Pichia membranifaciens were selected in a previous work for their biocontrol effec- tiveness against Penicillium expansum and Botrytis cinerea, the causal agents of blue and grey mold of pear fruits. The objective of this study was to determine the effects of different nutrient or additives on the growth of the two antagonist yeasts and of the two fungal pathogens in order to develop a rational selection based on nutritional profiles to be used in biocontrol enhancement of decay in pear fruits. Twenty-six different nutrient sources including 18 amino acids, five sugars, three inorganic nitrogen and one iron source were tested in vitro for their effect on the growth of the two yeasts and the two pathogens. Nutrients that promoted the growth of the yeasts and inhibited the growth of pathogens were applied with the antagonist to wounded fruits to evaluate their effect on enhancing biocontrol in cold storage. In general, the effect of each additive was specific to the yeasts and pathogens used in the in situ assays. The combination of the yeast and some additives resulted in a sig- nificantly higher activity with respect to the single treatments applied separately, producing synergistic effects. The effect of the exogenous application of CaCl2 and chitosan together with the antagonist yeasts was also evaluated against the two pathogens. The most effective mixtures were the CaCl2 with the two antagonist yeasts. Our research demonstrates that manipulating the chemical environment by adding several amino acid and/or Cl2Ca results in improved antagonistic activity of Vishniacozyma victoriae and Pichia membranifaciens against two postharvest diseases of pear fruits.
format Articulo
article
acceptedVersion
author Gramisci, Betina Rocío
Lutz, María Cecilia
Lopes, Christian Ariel
Sangorrin, Marcela Paula
author_facet Gramisci, Betina Rocío
Lutz, María Cecilia
Lopes, Christian Ariel
Sangorrin, Marcela Paula
author_sort Gramisci, Betina Rocío
title Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_short Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_full Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_fullStr Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_full_unstemmed Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_sort enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
publisher Elsevier
publishDate 2018
url https://rdi.uncoma.edu.ar/handle/uncomaid/19296
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