Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy

Exposure to environmental chemicals during pregnancy, including organophosphate pesticides, can affect the health of both the mother and the fetus, and have repercussions later in life. The present study aimed to determine whether the A-esterases paraoxonases (PON) and the B-esterases carboxylestera...

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Autores principales: Rodriguez, Piuque M., Vera, Berta, Burgos, Carolina, Gimenez, Gustavo, Miglioranza, Karina S.B., Ramirez, Cristina L., Lavalle, Andrea, Ondarza, Paola M., Guiñazú, Natalia L.
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Chlorpyrifos
Placenta
Esterases
Environmental exposure
Carboxylesterases
Paraoxonases
https://purl.org/becyt/ford/3.3
Ciencias Biomédicas
Artículos
Acceso en línea:https://rdi.uncoma.edu.ar/handle/uncomaid/19045
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-19045
record_format dspace
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Chlorpyrifos
Placenta
Esterases
Environmental exposure
Carboxylesterases
Paraoxonases
https://purl.org/becyt/ford/3.3
Ciencias Biomédicas
Artículos
spellingShingle Chlorpyrifos
Placenta
Esterases
Environmental exposure
Carboxylesterases
Paraoxonases
https://purl.org/becyt/ford/3.3
Ciencias Biomédicas
Artículos
Rodriguez, Piuque M.
Vera, Berta
Burgos, Carolina
Gimenez, Gustavo
Miglioranza, Karina S.B.
Ramirez, Cristina L.
Lavalle, Andrea
Ondarza, Paola M.
Guiñazú, Natalia L.
Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
topic_facet Chlorpyrifos
Placenta
Esterases
Environmental exposure
Carboxylesterases
Paraoxonases
https://purl.org/becyt/ford/3.3
Ciencias Biomédicas
Artículos
description Exposure to environmental chemicals during pregnancy, including organophosphate pesticides, can affect the health of both the mother and the fetus, and have repercussions later in life. The present study aimed to determine whether the A-esterases paraoxonases (PON) and the B-esterases carboxylesterases (CES) are modulated in the placenta of pregnant women residing in an intensive pesticide use scenario. A total of 104 healthy pregnant women were recruited between 2018 and 2022 and were classified according to their residential settings in rural (RG) and urban (UG) groups. Chlorpyrifos (CP) level in the placenta was determined by GC-ECD, and confirmed by GC-MS. To analyze possible impacts in esterases, the CES and PON activity, mRNA transcript and protein expression levels were studied. Significantly higher CP levels were detected in RG vs UG. Also, CES activity determined with 1-naphthyl acetate substrate was significantly lower in RG vs UG. In contrast, PON arylesterase and lactonase activities were up modulated in RG vs UG. Likewise, mRNA transcript levels of CES1, CES2 and PON2 were upregulated in the RG along with increases in CES2 and PON2 protein expressions. Moreover, a positive significant correlation was determined between CP concentration and CES1 and CES2 mRNA levels. Rural samples showed elevated CP concentrations and alterations in esterases, which elucidate the impact of CP exposure in mRNA CES and PON regulation. These findings highlight the need for further investigation into the effects of pesticide exposure during pregnancy and to deepen the knowledge about the function that esterases play in the placenta.
format Articulo
article
acceptedVersion
author Rodriguez, Piuque M.
Vera, Berta
Burgos, Carolina
Gimenez, Gustavo
Miglioranza, Karina S.B.
Ramirez, Cristina L.
Lavalle, Andrea
Ondarza, Paola M.
Guiñazú, Natalia L.
author_facet Rodriguez, Piuque M.
Vera, Berta
Burgos, Carolina
Gimenez, Gustavo
Miglioranza, Karina S.B.
Ramirez, Cristina L.
Lavalle, Andrea
Ondarza, Paola M.
Guiñazú, Natalia L.
author_sort Rodriguez, Piuque M.
title Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_short Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_full Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_fullStr Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_full_unstemmed Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_sort expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
publisher Elsevier
publishDate 2025
url https://rdi.uncoma.edu.ar/handle/uncomaid/19045
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spelling I22-R178-uncomaid-190452025-10-15T11:17:25Z Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy Rodriguez, Piuque M. Vera, Berta Burgos, Carolina Gimenez, Gustavo Miglioranza, Karina S.B. Ramirez, Cristina L. Lavalle, Andrea Ondarza, Paola M. Guiñazú, Natalia L. Chlorpyrifos Placenta Esterases Environmental exposure Carboxylesterases Paraoxonases https://purl.org/becyt/ford/3.3 Ciencias Biomédicas Artículos Exposure to environmental chemicals during pregnancy, including organophosphate pesticides, can affect the health of both the mother and the fetus, and have repercussions later in life. The present study aimed to determine whether the A-esterases paraoxonases (PON) and the B-esterases carboxylesterases (CES) are modulated in the placenta of pregnant women residing in an intensive pesticide use scenario. A total of 104 healthy pregnant women were recruited between 2018 and 2022 and were classified according to their residential settings in rural (RG) and urban (UG) groups. Chlorpyrifos (CP) level in the placenta was determined by GC-ECD, and confirmed by GC-MS. To analyze possible impacts in esterases, the CES and PON activity, mRNA transcript and protein expression levels were studied. Significantly higher CP levels were detected in RG vs UG. Also, CES activity determined with 1-naphthyl acetate substrate was significantly lower in RG vs UG. In contrast, PON arylesterase and lactonase activities were up modulated in RG vs UG. Likewise, mRNA transcript levels of CES1, CES2 and PON2 were upregulated in the RG along with increases in CES2 and PON2 protein expressions. Moreover, a positive significant correlation was determined between CP concentration and CES1 and CES2 mRNA levels. Rural samples showed elevated CP concentrations and alterations in esterases, which elucidate the impact of CP exposure in mRNA CES and PON regulation. These findings highlight the need for further investigation into the effects of pesticide exposure during pregnancy and to deepen the knowledge about the function that esterases play in the placenta. Fil: Rodriguez, Piuque M. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y la Salud. Departamento de Ciencias del Ambiente; Argentina. Fil: Rodriguez, Piuque M. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. Fil: Vera, Berta. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina. Fil: Vera, Berta. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. Fil: Burgos, Carolina. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. Fil: Gimenez, Gustavo. Universidad Nacional del Comahue. Facultad de Economía. Departamento de Estadística; Argentina. Fil: Miglioranza, Karina S.B. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Fil: Miglioranza, Karina S.B. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología y Contaminación Ambiental; Argentina. Fil: Ramirez, Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química y Bioquímica; Argentina. Fil: Lavalle, Andrea. Universidad Nacional del Comahue. Facultad de Economía. Departamento de Estadística; Argentina. Fil: Ondarza, Paola M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Fil: Ondarza, Paola M. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología y Contaminación Ambiental; Argentina. Fil: Giñazú, Natalia L. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y la Salud. Departamento de Ciencias del Ambiente; Argentina. Fil: Giñazú, Natalia L. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. 2025-05-14 2025-10-07T17:41:12Z 2025-10-07T17:41:12Z Articulo article acceptedVersion 0147-6513 1090-2414 https://rdi.uncoma.edu.ar/handle/uncomaid/19045 eng https://doi.org/10.1016/j.ecoenv.2025.118285 Atribución-NoComercial-CompartirIgual 4.0 https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf pp.1-13 application/pdf Elsevier Ecotoxicology and Environmental Safety. Volume 298

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