Everything everywhere all at once? Disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus Larrea
The genus Larrea has an amphitropical distribution in North and South American deserts, and its phylogeny remains unresolved. This genus is conspicuous and specious within the Monte Desert, the largest, although understudied, southern South American dryland. Larrea presents an interesting case for...
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| Formato: | Articulo article acceptedVersion |
| Lenguaje: | Inglés |
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Adrian Brennan
2025
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| Acceso en línea: | https://rdi.uncoma.edu.ar/handle/uncomaid/18835 |
| Aporte de: |
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I22-R178-uncomaid-18835 |
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dspace |
| institution |
Universidad Nacional del Comahue |
| institution_str |
I-22 |
| repository_str |
R-178 |
| collection |
Repositorio Institucional UNCo |
| language |
Inglés |
| topic |
Chloroplast sequences Creosote bush Cryptic hybridization Hybrid swarm Monte Desert North and South American drylands Nuclear sequences Ciencias de la Tierra y Medio Ambiente |
| spellingShingle |
Chloroplast sequences Creosote bush Cryptic hybridization Hybrid swarm Monte Desert North and South American drylands Nuclear sequences Ciencias de la Tierra y Medio Ambiente Quiroga, María Paula Castello, Lucia V. Tadey, Mariana Márquez, Sebastián Premoli, Andrea C. Souto, Cintia P. Everything everywhere all at once? Disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus Larrea |
| topic_facet |
Chloroplast sequences Creosote bush Cryptic hybridization Hybrid swarm Monte Desert North and South American drylands Nuclear sequences Ciencias de la Tierra y Medio Ambiente |
| description |
The genus Larrea has an amphitropical distribution in North and South American deserts, and its phylogeny remains unresolved. This
genus is conspicuous and specious within the Monte Desert, the largest, although understudied, southern South American dryland. Larrea presents an interesting case for phylogenetic studies due to its paternally inherited chloroplasts, its species hybridize in nature, and although nominal species are morphologically distinct, hybrids might be cryptic. We analysed ITS2 nuclear (nDNA) and rbcL chloroplast (cpDNA) sequences
of the bifoliolate section, Bifolium, including L. tridentata (Lt) from North America, and its South American congeners: L. cuneifolia (Lc) and
L. divaricata (Ld), and sequences of the multifoliolate Larrea section: L. ameghinoi (La), L. nitida (Ln), and a morphological hybrid swarm. We
aligned and analysed sequences from 111 individuals collected at 31 populations sampled along the range of each species. The nDNA revealed
56 haplotypes, and median-joining and maximum likelihood reconstructions provided clear separation among species and suggested hybridization between Lc-Ld. The nuclear phylogeny showed that the section Larrea diverged earlier than Bifolium, within which Lc diverged first,
meanwhile, consistent with previous studies, Lt forms a monophyletic group sister to Ld. Comparatively, cpDNA was less variable, with only
six haplotypes shared between Ln-Lc and Ln-La, and rarely between Ld-Lc. Our results emphasize the significance of separately considering
nuclear and plastid evolutionary signals when reconstructing unresolved relationships. While nuclear markers clarified phylogenetic relationships
and cryptic hybridization among Larrea species, the chloroplast revealed the retention of widespread ancient polymorphisms, which were conserved in populations of distinct species. Each marker provided insights into particular evolutionary patterns, highlighting that genetic variation
may be more influenced by hybridization and mode of chloroplast inheritance than previously recognized. |
| format |
Articulo article acceptedVersion |
| author |
Quiroga, María Paula Castello, Lucia V. Tadey, Mariana Márquez, Sebastián Premoli, Andrea C. Souto, Cintia P. |
| author_facet |
Quiroga, María Paula Castello, Lucia V. Tadey, Mariana Márquez, Sebastián Premoli, Andrea C. Souto, Cintia P. |
| author_sort |
Quiroga, María Paula |
| title |
Everything everywhere all at once? Disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus Larrea |
| title_short |
Everything everywhere all at once? Disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus Larrea |
| title_full |
Everything everywhere all at once? Disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus Larrea |
| title_fullStr |
Everything everywhere all at once? Disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus Larrea |
| title_full_unstemmed |
Everything everywhere all at once? Disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus Larrea |
| title_sort |
everything everywhere all at once? disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus larrea |
| publisher |
Adrian Brennan |
| publishDate |
2025 |
| url |
https://rdi.uncoma.edu.ar/handle/uncomaid/18835 |
| work_keys_str_mv |
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1840858878195007488 |
| spelling |
I22-R178-uncomaid-188352025-07-22T17:53:04Z Everything everywhere all at once? Disentangling the long-lasting riddle of phylogenetic relationships and cryptic hybridization in the amphitropical genus Larrea Quiroga, María Paula Castello, Lucia V. Tadey, Mariana Márquez, Sebastián Premoli, Andrea C. Souto, Cintia P. Chloroplast sequences Creosote bush Cryptic hybridization Hybrid swarm Monte Desert North and South American drylands Nuclear sequences Ciencias de la Tierra y Medio Ambiente The genus Larrea has an amphitropical distribution in North and South American deserts, and its phylogeny remains unresolved. This genus is conspicuous and specious within the Monte Desert, the largest, although understudied, southern South American dryland. Larrea presents an interesting case for phylogenetic studies due to its paternally inherited chloroplasts, its species hybridize in nature, and although nominal species are morphologically distinct, hybrids might be cryptic. We analysed ITS2 nuclear (nDNA) and rbcL chloroplast (cpDNA) sequences of the bifoliolate section, Bifolium, including L. tridentata (Lt) from North America, and its South American congeners: L. cuneifolia (Lc) and L. divaricata (Ld), and sequences of the multifoliolate Larrea section: L. ameghinoi (La), L. nitida (Ln), and a morphological hybrid swarm. We aligned and analysed sequences from 111 individuals collected at 31 populations sampled along the range of each species. The nDNA revealed 56 haplotypes, and median-joining and maximum likelihood reconstructions provided clear separation among species and suggested hybridization between Lc-Ld. The nuclear phylogeny showed that the section Larrea diverged earlier than Bifolium, within which Lc diverged first, meanwhile, consistent with previous studies, Lt forms a monophyletic group sister to Ld. Comparatively, cpDNA was less variable, with only six haplotypes shared between Ln-Lc and Ln-La, and rarely between Ld-Lc. Our results emphasize the significance of separately considering nuclear and plastid evolutionary signals when reconstructing unresolved relationships. While nuclear markers clarified phylogenetic relationships and cryptic hybridization among Larrea species, the chloroplast revealed the retention of widespread ancient polymorphisms, which were conserved in populations of distinct species. Each marker provided insights into particular evolutionary patterns, highlighting that genetic variation may be more influenced by hybridization and mode of chloroplast inheritance than previously recognized. Fil: Quiroga, María Paula. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Quiroga, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Castello, Lucia V. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Castello, Lucia V. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Tadey, Mariana. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Tadey, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Márquez, Sebastián. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Márquez, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Premoli, Andrea C. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Premoli, Andrea C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Souto, Cintia P. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Souto, Cintia P. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. 2025 2025-07-04T15:44:37Z 2025-07-04T15:44:37Z Articulo article acceptedVersion https://rdi.uncoma.edu.ar/handle/uncomaid/18835 eng https://doi.org/10.1093/aobpla/plaf024 Atribución-NoComercial-CompartirIgual 4.0 https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/pdf Adrian Brennan Oxford Academic AoB PLANTS, Volume 17, Issue 3, June 2025, plaf024 |