Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain

Phaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is o...

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Autores principales: Moliné, Martín, Libkind, Diego, van Broock, María Rosa
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: Springer 2024
Materias:
Phaa rhodozyma
Mycosporine-glutaminol-glucoside
Carotenoids
Astaxanthin
Ciencias de la Tierra y Medio Ambiente
Acceso en línea:https://rdi.uncoma.edu.ar/handle/uncomaid/18810
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-18810
record_format dspace
spelling I22-R178-uncomaid-188102025-07-02T14:03:30Z Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain Moliné, Martín Libkind, Diego van Broock, María Rosa Phaa rhodozyma Mycosporine-glutaminol-glucoside Carotenoids Astaxanthin Ciencias de la Tierra y Medio Ambiente Phaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is of great biotechnological relevance for its incorporation into natural sunscreens. However, the industrial exploitation has been limited to the production of astaxanthin in small quantities. On the other hand, the accumulation of MGG in P. rhodozyma was recently reported and could add value to the simultaneous production of both metabolites. In this work, we obtain a mutant strain that overproduces both compounds, furthermore we determined how the accumulation of each is affected by the carbon-to-nitrogen ratio and six biotic and abiotic factors. The mutant obtained produces 159% more astaxanthin and 220% more MGG than the parental strain. Furthermore, we establish that the carotenoids accumulate during the exponential growth phase while MGG accumulates during the stationary phase. The carbon-to-nitrogen ratio affects each metabolite differently, high ratios favoring carotenoid accumulation while low ratios favoring MGG accumulation. Finally, the accumulation of both metabolites is stimulated only by photosynthetically active radiation and low concentrations of hydrogen peroxide. The mutant strain obtained is the first hyper-productive mutant capable of accumulating high concentrations of MGG and astaxanthin described to date. The characterization of how both compounds accumulate during growth and the factors that stimulate their accumulation, are the first steps toward the future commercial exploitation of strains for the simultaneous production of two biotechnologically important metabolites. Fil: Moliné, Martín. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina. Fil: Libkind, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina. 2024 2025-06-26T13:45:16Z 2025-06-26T13:45:16Z Articulo article acceptedVersion https://rdi.uncoma.edu.ar/handle/uncomaid/18810 eng https://doi.org/10.21203/rs.3.rs-3495924/v1 Atribución-NoComercial-CompartirIgual 4.0 https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf pp.1-27 application/pdf Springer World Journal of Microbiology and Biotechnology.
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Phaa rhodozyma
Mycosporine-glutaminol-glucoside
Carotenoids
Astaxanthin
Ciencias de la Tierra y Medio Ambiente
spellingShingle Phaa rhodozyma
Mycosporine-glutaminol-glucoside
Carotenoids
Astaxanthin
Ciencias de la Tierra y Medio Ambiente
Moliné, Martín
Libkind, Diego
van Broock, María Rosa
Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
topic_facet Phaa rhodozyma
Mycosporine-glutaminol-glucoside
Carotenoids
Astaxanthin
Ciencias de la Tierra y Medio Ambiente
description Phaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is of great biotechnological relevance for its incorporation into natural sunscreens. However, the industrial exploitation has been limited to the production of astaxanthin in small quantities. On the other hand, the accumulation of MGG in P. rhodozyma was recently reported and could add value to the simultaneous production of both metabolites. In this work, we obtain a mutant strain that overproduces both compounds, furthermore we determined how the accumulation of each is affected by the carbon-to-nitrogen ratio and six biotic and abiotic factors. The mutant obtained produces 159% more astaxanthin and 220% more MGG than the parental strain. Furthermore, we establish that the carotenoids accumulate during the exponential growth phase while MGG accumulates during the stationary phase. The carbon-to-nitrogen ratio affects each metabolite differently, high ratios favoring carotenoid accumulation while low ratios favoring MGG accumulation. Finally, the accumulation of both metabolites is stimulated only by photosynthetically active radiation and low concentrations of hydrogen peroxide. The mutant strain obtained is the first hyper-productive mutant capable of accumulating high concentrations of MGG and astaxanthin described to date. The characterization of how both compounds accumulate during growth and the factors that stimulate their accumulation, are the first steps toward the future commercial exploitation of strains for the simultaneous production of two biotechnologically important metabolites.
format Articulo
article
acceptedVersion
author Moliné, Martín
Libkind, Diego
van Broock, María Rosa
author_facet Moliné, Martín
Libkind, Diego
van Broock, María Rosa
author_sort Moliné, Martín
title Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_short Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_full Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_fullStr Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_full_unstemmed Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_sort two at once: simultaneous increased production of astaxanthin and mycosporines in a single batch culture using a phaffia rhodozyma mutant strain
publisher Springer
publishDate 2024
url https://rdi.uncoma.edu.ar/handle/uncomaid/18810
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