Effects of acute arsenic exposure in two different populations of Hyalella curvispina amphipods from North Patagonia Argentina

Arsenic (As) is a toxic metalloid present in high levels in diverse regions of Argentina. The aim of this study was to determine acute As-mediated toxicity in two different populations of autochthonous Hyalella curvispina amphipods from a reference site (LB) and an agricultural one (FO) within North...

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Autores principales: Kirilovsky, Eva R., Anguiano, Olga L., Bongiovanni, Guillermina A., Ferrari, Ana
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: Taylor & Francis 2021
Materias:
Arsenic toxicity
Hyalella curvispina
cholinesterase
Antioxidant enzymes
GSH content
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Acceso en línea:https://rdi.uncoma.edu.ar/handle/uncomaid/18315
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-18315
record_format dspace
spelling I22-R178-uncomaid-183152025-03-06T14:31:26Z Effects of acute arsenic exposure in two different populations of Hyalella curvispina amphipods from North Patagonia Argentina Kirilovsky, Eva R. Anguiano, Olga L. Bongiovanni, Guillermina A. Ferrari, Ana Arsenic toxicity Hyalella curvispina cholinesterase Antioxidant enzymes GSH content Ciencias de la Tierra y Medio Ambiente Ciencias Biomédicas Arsenic (As) is a toxic metalloid present in high levels in diverse regions of Argentina. The aim of this study was to determine acute As-mediated toxicity in two different populations of autochthonous Hyalella curvispina amphipods from a reference site (LB) and an agricultural one (FO) within North Patagonia Argentina. Previously, both populations exhibited significant differences in pesticide susceptibility. Lab assays were performed to determine acute lethal concentrations, as well as some biochemical parameters. Lethal concentration (LC50) values obtained after 48 and 96 hr As exposure were not significantly different between these populations, although FO amphipods appeared slightly less susceptible. LC50-48 hr values were 3.33 and 3.92 mg/L As, while LC50-96 hr values were 1.76 and 2.14 mg/L As for LB and FO amphipods. The no observed effect concentration (NOEC) values were 0.5 mg/L As. Cholinesterase (ChE) activity was significantly diminished by As acute exposure (0.5–1.5 mg/L As), indicative of a significant neurotoxic action for this metalloid in both amphipod populations. Activities of catalase (CAT) and glutathione S-transferase (GST) and levels of reduced glutathione (GSH) were differentially altered following As exposure. CAT activity was increased after 96 hr As exposure. GST activity and GSH levels were significantly elevated followed by either a decrease or a return to control values after 96 hr treatment. However, additional studies are necessary to understand the mechanisms underlying the As-mediated oxidative effects in H. curvispina. Our findings suggest that measurement of ChE activity in H. curvispina amphipods might serve as a useful biomarker of As exposure and effect. Fil: Kirilovsky, Eva R. Universidad Nacional del Comahue. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Fil: Anguiano, Olga L. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Fil: Bongiovanni. Guillermina A. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Ferrari, Ana. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina. 2021-09-08 2024-11-19T15:06:35Z 2024-11-19T15:06:35Z Articulo article acceptedVersion 1528-7394 https://rdi.uncoma.edu.ar/handle/uncomaid/18315 eng https://www.tandfonline.com/doi/full/10.1080/15287394.2021.1975589 Atribución-NoComercial-CompartirIgual 4.0 https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf pp. 1-19 application/pdf ARG Taylor & Francis Journal of Toxicology and Environmental Health, Part A Current Issues. Vol. 85 (2022)
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Arsenic toxicity
Hyalella curvispina
cholinesterase
Antioxidant enzymes
GSH content
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
spellingShingle Arsenic toxicity
Hyalella curvispina
cholinesterase
Antioxidant enzymes
GSH content
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Kirilovsky, Eva R.
Anguiano, Olga L.
Bongiovanni, Guillermina A.
Ferrari, Ana
Effects of acute arsenic exposure in two different populations of Hyalella curvispina amphipods from North Patagonia Argentina
topic_facet Arsenic toxicity
Hyalella curvispina
cholinesterase
Antioxidant enzymes
GSH content
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
description Arsenic (As) is a toxic metalloid present in high levels in diverse regions of Argentina. The aim of this study was to determine acute As-mediated toxicity in two different populations of autochthonous Hyalella curvispina amphipods from a reference site (LB) and an agricultural one (FO) within North Patagonia Argentina. Previously, both populations exhibited significant differences in pesticide susceptibility. Lab assays were performed to determine acute lethal concentrations, as well as some biochemical parameters. Lethal concentration (LC50) values obtained after 48 and 96 hr As exposure were not significantly different between these populations, although FO amphipods appeared slightly less susceptible. LC50-48 hr values were 3.33 and 3.92 mg/L As, while LC50-96 hr values were 1.76 and 2.14 mg/L As for LB and FO amphipods. The no observed effect concentration (NOEC) values were 0.5 mg/L As. Cholinesterase (ChE) activity was significantly diminished by As acute exposure (0.5–1.5 mg/L As), indicative of a significant neurotoxic action for this metalloid in both amphipod populations. Activities of catalase (CAT) and glutathione S-transferase (GST) and levels of reduced glutathione (GSH) were differentially altered following As exposure. CAT activity was increased after 96 hr As exposure. GST activity and GSH levels were significantly elevated followed by either a decrease or a return to control values after 96 hr treatment. However, additional studies are necessary to understand the mechanisms underlying the As-mediated oxidative effects in H. curvispina. Our findings suggest that measurement of ChE activity in H. curvispina amphipods might serve as a useful biomarker of As exposure and effect.
format Articulo
article
acceptedVersion
author Kirilovsky, Eva R.
Anguiano, Olga L.
Bongiovanni, Guillermina A.
Ferrari, Ana
author_facet Kirilovsky, Eva R.
Anguiano, Olga L.
Bongiovanni, Guillermina A.
Ferrari, Ana
author_sort Kirilovsky, Eva R.
title Effects of acute arsenic exposure in two different populations of Hyalella curvispina amphipods from North Patagonia Argentina
title_short Effects of acute arsenic exposure in two different populations of Hyalella curvispina amphipods from North Patagonia Argentina
title_full Effects of acute arsenic exposure in two different populations of Hyalella curvispina amphipods from North Patagonia Argentina
title_fullStr Effects of acute arsenic exposure in two different populations of Hyalella curvispina amphipods from North Patagonia Argentina
title_full_unstemmed Effects of acute arsenic exposure in two different populations of Hyalella curvispina amphipods from North Patagonia Argentina
title_sort effects of acute arsenic exposure in two different populations of hyalella curvispina amphipods from north patagonia argentina
publisher Taylor & Francis
publishDate 2021
url https://rdi.uncoma.edu.ar/handle/uncomaid/18315
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