Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi

The Phaffia rhodozyma UCD 67-385 genome harbors a 7873 bp cluster containing DDGS, OMT, and ATPG, encoding 2-desmethy-4-deoxygadusol synthase, O-methyl transferase, and ATP- grasp ligase, respectively, of the mycosporine glutaminol (MG) biosynthesis pathway. Homozy- gous deletion mutants of the enti...

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Autores principales: Sepúlveda, Dionisia, Campusano, Sebastián, Moliné, Martín, Barahona, Salvador, Baeza, Marcelo, Alcaíno, Jennifer, Colabella, Fernando, Urzúa, Blanca, Libkind, Diego, Cifuentes, Víctor
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: MDPI 2023
Materias:
Phaffia
Mycosporine
Genes cluster
Secondary metabolite
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Acceso en línea:http://rdi.uncoma.edu.ar/handle/uncomaid/17451
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-17451
record_format dspace
spelling I22-R178-uncomaid-174512023-10-12T10:30:45Z Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi Sepúlveda, Dionisia Campusano, Sebastián Moliné, Martín Barahona, Salvador Baeza, Marcelo Alcaíno, Jennifer Colabella, Fernando Urzúa, Blanca Libkind, Diego Cifuentes, Víctor Phaffia Mycosporine Genes cluster Secondary metabolite Ciencias de la Tierra y Medio Ambiente Ciencias Biomédicas The Phaffia rhodozyma UCD 67-385 genome harbors a 7873 bp cluster containing DDGS, OMT, and ATPG, encoding 2-desmethy-4-deoxygadusol synthase, O-methyl transferase, and ATP- grasp ligase, respectively, of the mycosporine glutaminol (MG) biosynthesis pathway. Homozy- gous deletion mutants of the entire cluster, single-gene mutants, and the ∆ddgs−/−;∆omt−/− and ∆omt−/−;∆atpg−/− double-gene mutants did not produce mycosporines. However, ∆atpg−/− accu- mulated the intermediate 4-deoxygadusol. Heterologous expression of the DDGS and OMT or DDGS, OMT, and ATPG cDNAs in Saccharomyces cerevisiae led to 4-deoxygadusol or MG production, respec- tively. Genetic integration of the complete cluster into the genome of the non-mycosporine-producing CBS 6938 wild-type strain resulted in a transgenic strain (CBS 6938_MYC) that produced MG and my- cosporine glutaminol glucoside. These results indicate the function of DDGS, OMT, and ATPG in the mycosporine biosynthesis pathway. The transcription factor gene mutants ∆mig1−/−, ∆cyc8−/−, and ∆opi1−/− showed upregulation, ∆rox1−/− and ∆skn7−/− showed downregulation, and ∆tup6−/− and ∆yap6−/− showed no effect on mycosporinogenesis in glucose-containing medium. Finally, comparative analysis of the cluster sequences in several P. rhodozyma strains and the four newly described species of the genus showed the phylogenetic relationship of the P. rhodozyma strains and their differentiation from the other species of the genus Phaffia. Fil: Sepúlveda, Dionisia. Universidad de Chile. Facultad de Ciencias. Departamento de Ciencias Ecológicas; Chile. Fil: Campusano, Sebastián. Universidad de Chile. Facultad de Ciencias. Departamento de Ciencias Ecológicas; Chile. Fil: Moliné, Martín. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Moliné, Martín. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Moliné, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Barahona, Salvador. Universidad de Chile. Facultad de Ciencias. Departamento de Ciencias Ecológicas; Chile. Fil: Baeza, Marcelo. Universidad de Chile. Facultad de Ciencias. Departamento de Ciencias Ecológicas; Chile. Fil: Alcaíno, Jennifer. Universidad de Chile. Facultad de Ciencias. Departamento de Ciencias Ecológicas; Chile. Fil: Colabella, Fernando. Independent Researcher Bariloche; Argentina. Fil: Urzúa, Blanca. Universidad de Chile. Facultad de Odontología. Instituto de Investigación en Ciencias Odontológicas; Chile. Fil: Libkind, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Libkind, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Cifuentes, Víctor. Universidad de Chile. Facultad de Ciencias. Departamento de Ciencias Ecológicas; Chile. 2023 2023-10-11T17:15:19Z 2023-10-11T17:15:19Z Articulo article acceptedVersion http://rdi.uncoma.edu.ar/handle/uncomaid/17451 eng https://doi.org/10.3390/ijms24065930 Atribución-NoComercial-CompartirIgual 2.5 Argentina https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ application/pdf pp. 1-19 application/pdf MDPI International Journal of Molecular Sciences. 2023, 24, 5930
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Phaffia
Mycosporine
Genes cluster
Secondary metabolite
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
spellingShingle Phaffia
Mycosporine
Genes cluster
Secondary metabolite
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Sepúlveda, Dionisia
Campusano, Sebastián
Moliné, Martín
Barahona, Salvador
Baeza, Marcelo
Alcaíno, Jennifer
Colabella, Fernando
Urzúa, Blanca
Libkind, Diego
Cifuentes, Víctor
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi
topic_facet Phaffia
Mycosporine
Genes cluster
Secondary metabolite
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
description The Phaffia rhodozyma UCD 67-385 genome harbors a 7873 bp cluster containing DDGS, OMT, and ATPG, encoding 2-desmethy-4-deoxygadusol synthase, O-methyl transferase, and ATP- grasp ligase, respectively, of the mycosporine glutaminol (MG) biosynthesis pathway. Homozy- gous deletion mutants of the entire cluster, single-gene mutants, and the ∆ddgs−/−;∆omt−/− and ∆omt−/−;∆atpg−/− double-gene mutants did not produce mycosporines. However, ∆atpg−/− accu- mulated the intermediate 4-deoxygadusol. Heterologous expression of the DDGS and OMT or DDGS, OMT, and ATPG cDNAs in Saccharomyces cerevisiae led to 4-deoxygadusol or MG production, respec- tively. Genetic integration of the complete cluster into the genome of the non-mycosporine-producing CBS 6938 wild-type strain resulted in a transgenic strain (CBS 6938_MYC) that produced MG and my- cosporine glutaminol glucoside. These results indicate the function of DDGS, OMT, and ATPG in the mycosporine biosynthesis pathway. The transcription factor gene mutants ∆mig1−/−, ∆cyc8−/−, and ∆opi1−/− showed upregulation, ∆rox1−/− and ∆skn7−/− showed downregulation, and ∆tup6−/− and ∆yap6−/− showed no effect on mycosporinogenesis in glucose-containing medium. Finally, comparative analysis of the cluster sequences in several P. rhodozyma strains and the four newly described species of the genus showed the phylogenetic relationship of the P. rhodozyma strains and their differentiation from the other species of the genus Phaffia.
format Articulo
article
acceptedVersion
author Sepúlveda, Dionisia
Campusano, Sebastián
Moliné, Martín
Barahona, Salvador
Baeza, Marcelo
Alcaíno, Jennifer
Colabella, Fernando
Urzúa, Blanca
Libkind, Diego
Cifuentes, Víctor
author_facet Sepúlveda, Dionisia
Campusano, Sebastián
Moliné, Martín
Barahona, Salvador
Baeza, Marcelo
Alcaíno, Jennifer
Colabella, Fernando
Urzúa, Blanca
Libkind, Diego
Cifuentes, Víctor
author_sort Sepúlveda, Dionisia
title Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi
title_short Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi
title_full Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi
title_fullStr Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi
title_full_unstemmed Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi
title_sort unraveling the molecular basis of mycosporine biosynthesis in fungi
publisher MDPI
publishDate 2023
url http://rdi.uncoma.edu.ar/handle/uncomaid/17451
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