Interacción de un tensioactivo biocompatible con estructuras de interés farmacológico

In this thesis both the behavior of ascorbyl palmitate (Asc16) in solution and monolayers and the behavior of amiodarone (AMI) in solution were studied. Finally, liposomes with Asc16 and AMI were developed. The behavior of aqueous systems of Asc16 with and without different proportions of polyethyle...

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Detalles Bibliográficos
Autor principal: Benedini, Luciano Alejandro
Otros Autores: Schulz, Pablo Carlos
Formato: tesis doctoral
Lenguaje:Español
Publicado: 2012
Materias:
Amiodarona
Palmitato de ascorbilo
Cristales líquidos
Acceso en línea:http://repositoriodigital.uns.edu.ar/handle/123456789/2294
Aporte de:
Repositorio Institucional Universidad Nacional del Sur (UNS) de Universidad Nacional del Sur
id I20-R126123456789-2294
record_format dspace
institution Universidad Nacional del Sur
institution_str I-20
repository_str R-126
collection Repositorio Institucional Universidad Nacional del Sur (UNS)
language Español
orig_language_str_mv spa
topic Amiodarona
Palmitato de ascorbilo
Cristales líquidos
spellingShingle Amiodarona
Palmitato de ascorbilo
Cristales líquidos
Benedini, Luciano Alejandro
Interacción de un tensioactivo biocompatible con estructuras de interés farmacológico
topic_facet Amiodarona
Palmitato de ascorbilo
Cristales líquidos
description In this thesis both the behavior of ascorbyl palmitate (Asc16) in solution and monolayers and the behavior of amiodarone (AMI) in solution were studied. Finally, liposomes with Asc16 and AMI were developed. The behavior of aqueous systems of Asc16 with and without different proportions of polyethylene glycol 400 (PEG400) were described. Differential scanning calorimetry (DSC) and polarizing microscopy (MOP) were both used to draw four phase diagrams. The aqueous system below C ~ 0.48 w/w shows hydrated crystals in an isotropic liquid, which become a lamellar liquid crystal when heated. Above this concentration, a cubic liquid crystal appears before the lamellar mesophase. Two different kind of water, other than bulk, using DSC was detected: a first hydration shell, which does not melt and is then undetectable though plausible to infer with DSC; and a second hydration layer. The number of water molecules of this shell decreases as the surfactant concentration increases. Asc16 systems with different propor-tions of PEG400 and water show similar textures to those in the aqueous system. The addition of PEG400 to the Asc16water system shifts the limits of the liquid crystalline domains to lower temperature and surfactant concentration. Neither phases described in this work nor water behavior in Asc16-water systems were found in the literature. The surface behavior of Asc16 was studied by Langmuir monolayers (LM) and Brewster angle microscopy (BAM). Depending on subpha-se conditions Asc16 forms stable monolayers at room tempera-ture and shows phase transition from a liquidexpanded to a liquid-condensed or a crystalline phase. Both condensed phases show preferential growth and small flowerlike domains depending on the charge density. Adsorption (AD) of Asc16 in lipids monolayers have shown that stable mixed monolayers of Asc16 and DMPC that have been formed. These three techni-ques (ML, BAM and AD) were not found to be previously used together to describe bidimensional behavior of Asc16. Low concentrations AMI aqueous systems by many techniques and at various temperatures was studied. The Krafft point (TK) was determined. A partial phase diagram showing the critical micelle concentration (cmc) dependence on temperature, the solubility and a transition between a coacervate and an unstable gel below TK was drawn as a function of tempera-ture. This diagram, with a comprehension of structures for-med close to the Krafft point, was not previously reported in the literature. Liposomes systems of DMPC with Asc16 and DMPC with Asc16 and AMI were studied by generalized pola-rization (GP), size particle and zeta potential determinations. Liposomes stability was analyzed by means of the Derjaguin-Landau-Vervey-Overbeek (DLVO) theory. GP determinations shown that the three different concentrations of Asc16 used in liposomes did not disturbed DMPC membrane organization. Furthermore, modified DLVO theory predicted liposomes stability. The addition of AMI to the liposomes of DMPC plus Asc16 did not significantly affect the aggregates size. Litera-ture reports about either the use of Asc16 to prevent adverse effects produced by AMI or the development of liposomes with AMI and the Asc16 were not found.
author2 Schulz, Pablo Carlos
author_facet Schulz, Pablo Carlos
Benedini, Luciano Alejandro
format tesis doctoral
author Benedini, Luciano Alejandro
author_sort Benedini, Luciano Alejandro
title Interacción de un tensioactivo biocompatible con estructuras de interés farmacológico
title_short Interacción de un tensioactivo biocompatible con estructuras de interés farmacológico
title_full Interacción de un tensioactivo biocompatible con estructuras de interés farmacológico
title_fullStr Interacción de un tensioactivo biocompatible con estructuras de interés farmacológico
title_full_unstemmed Interacción de un tensioactivo biocompatible con estructuras de interés farmacológico
title_sort interacción de un tensioactivo biocompatible con estructuras de interés farmacológico
publishDate 2012
url http://repositoriodigital.uns.edu.ar/handle/123456789/2294
work_keys_str_mv AT benedinilucianoalejandro interacciondeuntensioactivobiocompatibleconestructurasdeinteresfarmacologico
bdutipo_str Repositorios
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