Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
Stenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little is known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates the study of pathogenicity and virulence factors. Here, we present a strategy to identify such fact...
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| Autores principales: | , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2013
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/99915 https://ri.conicet.gov.ar/11336/22286 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067207 |
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I19-R120-10915-99915 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Stenotrophomonas Oportunista Multiresistencia Proteomica |
| spellingShingle |
Biología Stenotrophomonas Oportunista Multiresistencia Proteomica Ferrer Navarro, Mario Planell, Raquel Yero, Daniel Mongiardini, Elías Javier Torrent, Gerard Huedo, Pol Martinez, Paula Roher, Nerea Mackenzie, Simon Gibert, Isidre Daura, Xavier Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection |
| topic_facet |
Biología Stenotrophomonas Oportunista Multiresistencia Proteomica |
| description |
Stenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little is known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates the study of pathogenicity and virulence factors. Here, we present a strategy to identify such factors in new clinical isolates of S. maltophilia, incorporating an adultzebrafish model of S. maltophilia infection to evaluate relative virulence coupled to 2D difference gel electrophoresis to explore underlying differences in protein expression. In this study we report upon three recent clinical isolates and use the collection strain ATCC13637 as a reference. The adult-zebrafish model shows discrimination capacity, i.e. from very low to very high mortality rates, with clinical symptoms very similar to those observed in natural S. maltophilia infections in fish. Strain virulence correlates with resistance to human serum, in agreement with previous studies in mouse and rat and therefore supporting zebrafish as a replacement model. Despite its clinical origin, the collection strain ATCC13637 showed obvious signs of attenuation in zebrafish, with null mortality. Multilocus-sequence-typing analysis revealed that the most virulent strains, UV74 and M30, exhibit the strongest genetic similitude. Differential proteomic analysis led to the identification of 38 proteins with significantly different abundance in the three clinical strains relative to the reference strain. Orthologs of several of these proteins have been already reported to have a role in pathogenesis, virulence or resistance mechanisms thus supporting our strategy. Proof of concept is further provided by protein Ax21, whose abundance is shown here to be directly proportional to mortality in the zebrafish infection model. Indeed, recent studies have demonstrated that this protein is a quorum-sensing-related virulence factor. |
| format |
Articulo Articulo |
| author |
Ferrer Navarro, Mario Planell, Raquel Yero, Daniel Mongiardini, Elías Javier Torrent, Gerard Huedo, Pol Martinez, Paula Roher, Nerea Mackenzie, Simon Gibert, Isidre Daura, Xavier |
| author_facet |
Ferrer Navarro, Mario Planell, Raquel Yero, Daniel Mongiardini, Elías Javier Torrent, Gerard Huedo, Pol Martinez, Paula Roher, Nerea Mackenzie, Simon Gibert, Isidre Daura, Xavier |
| author_sort |
Ferrer Navarro, Mario |
| title |
Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection |
| title_short |
Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection |
| title_full |
Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection |
| title_fullStr |
Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection |
| title_full_unstemmed |
Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection |
| title_sort |
abundance of the quorum-sensing factor ax21 in four strains of stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection |
| publishDate |
2013 |
| url |
http://sedici.unlp.edu.ar/handle/10915/99915 https://ri.conicet.gov.ar/11336/22286 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067207 |
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Repositorios |
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1764820493432520708 |