Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization

Microbiologically influenced corrosion and souring of oilfield reservoirs are process frequently provoked by the sulphate-reducing bacteria. The most common method applied in the industry for preventing or controlling the deleterious effect caused by the presence of microorganisms is the addition of...

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Autores principales: Viera, Marisa Rosana, Terada, Claudia, Madrid, R. E., Felice, J. C., Del Panno, María Teresa
Formato: Objeto de conferencia
Lenguaje:Inglés
Publicado: 2016
Materias:
Química
biocides
biocorrosion
fluorescent in-situ
hybridization
sulphate-reducing bacteria
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99897
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-99897
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
biocides
biocorrosion
fluorescent in-situ
hybridization
sulphate-reducing bacteria
spellingShingle Química
biocides
biocorrosion
fluorescent in-situ
hybridization
sulphate-reducing bacteria
Viera, Marisa Rosana
Terada, Claudia
Madrid, R. E.
Felice, J. C.
Del Panno, María Teresa
Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization
topic_facet Química
biocides
biocorrosion
fluorescent in-situ
hybridization
sulphate-reducing bacteria
description Microbiologically influenced corrosion and souring of oilfield reservoirs are process frequently provoked by the sulphate-reducing bacteria. The most common method applied in the industry for preventing or controlling the deleterious effect caused by the presence of microorganisms is the addition of chemical agents (biocides) aimed at killing the microorganisms or inhibiting the microbial growth. Traditionally, biocide selection and testing are based on NACE standard TM0194 which implies the use of culturing for enumerating the bacteria surviving the treatment. To overcome culturing limitations, we used Fluorescent in-situ Hybridization to assist in the evaluation of biocides applied in water production treatment plants. Biocides were based on THPS (40% (B1); 75% (B2)) and 40% of a mixture (1/1, v/v) of THPS and benzalkonium chloride (B3) applied at two concentrations: 50 and 400 mg/L. The relation between the number of cells visualized with the fluorescent probes Eub338 and SRB385 (for eubacteria and SRB populations respectively) and the DAPI-stained cells (PR%) was used as an indication of the biocide efficiency. B1 and B3 gave a high PR% indicating that the chemical induced the metabolic cell activity. Only the highest B2 concentration showed effectiveness on eubacteria and SRB populations. Thus, through the application of FISH we were able to distinguish concentration effects of the THPS, discriminating sublethal from net inhibitory effects. The possibility of including FISH into the protocols for the control of the biocides in water treatment plants could improve the biocide selection and the adjustment of their concentration in order to maintain the water system with a low density of metabolically active cells. This would avoid the misuse of chemicals with their consequent economic and ecological impacts.
format Objeto de conferencia
Objeto de conferencia
author Viera, Marisa Rosana
Terada, Claudia
Madrid, R. E.
Felice, J. C.
Del Panno, María Teresa
author_facet Viera, Marisa Rosana
Terada, Claudia
Madrid, R. E.
Felice, J. C.
Del Panno, María Teresa
author_sort Viera, Marisa Rosana
title Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization
title_short Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization
title_full Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization
title_fullStr Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization
title_full_unstemmed Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization
title_sort evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/99897
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