Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies

Objective: Chagas disease constitutes a major public health problem in Latin America. Correctly designed pharmacokinetic, safety, and bioequivalence studies are desirable in order to fill the knowledge gaps that presently exist on available drugs. It is necessary to develop accurate, simple, reprodu...

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Autores principales: Marson, María Elena, Dana, Diego Dante, Altcheh, Jaime Marcelo, García Bournissen, Facundo, Mastrantonio Garrido, Guido Enrique
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Médicas
Benznidazole
HPLC
Urine
Pediatric clinical pharmacology
Chagas disease
Plasma
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99862
https://ri.conicet.gov.ar/11336/25466
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-99862
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Benznidazole
HPLC
Urine
Pediatric clinical pharmacology
Chagas disease
Plasma
spellingShingle Ciencias Médicas
Benznidazole
HPLC
Urine
Pediatric clinical pharmacology
Chagas disease
Plasma
Marson, María Elena
Dana, Diego Dante
Altcheh, Jaime Marcelo
García Bournissen, Facundo
Mastrantonio Garrido, Guido Enrique
Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies
topic_facet Ciencias Médicas
Benznidazole
HPLC
Urine
Pediatric clinical pharmacology
Chagas disease
Plasma
description Objective: Chagas disease constitutes a major public health problem in Latin America. Correctly designed pharmacokinetic, safety, and bioequivalence studies are desirable in order to fill the knowledge gaps that presently exist on available drugs. It is necessary to develop accurate, simple, reproducible, and sensitive high-performance liquid chromatography (HPLC)/UV methods for the quantization of benznidazole (BNZ) in human plasma and urine for clinical applications, specially in pediatric patients. Methods: Quantization of BNZ in human plasma involved freeze-drying and re-suspension in organic solvent followed by reverse phase HPLC with UV detection. Analysis of BNZ in urine involved liquid/liquid extraction followed by reverse phase HPLC with UV detection. RESULTS: Limits of quantization (LOQ) were 0.32 μg/ml for plasma and 5.2 μg/ml for urine. No metabolite interferences were showed in both methods. Conclusion: The LOQ of methods seems appropriate in pediatric clinical contexts. Both procedures were applied with good results, to the quantization of BNZ in plasma and urine of patients treated for Chagas disease.
format Articulo
Articulo
author Marson, María Elena
Dana, Diego Dante
Altcheh, Jaime Marcelo
García Bournissen, Facundo
Mastrantonio Garrido, Guido Enrique
author_facet Marson, María Elena
Dana, Diego Dante
Altcheh, Jaime Marcelo
García Bournissen, Facundo
Mastrantonio Garrido, Guido Enrique
author_sort Marson, María Elena
title Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies
title_short Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies
title_full Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies
title_fullStr Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies
title_full_unstemmed Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies
title_sort development of uv/hplc methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/99862
https://ri.conicet.gov.ar/11336/25466
work_keys_str_mv AT marsonmariaelena developmentofuvhplcmethodsforquantitativeanalysisofbenznidazoleinhumanplasmaandurineforapplicationinpediatricclinicalstudies
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