Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods

The aim of this study was to evaluate different immunochemical methods (Dot Blot, Immnoblotting and two different ELISA kits) for the detection of milk proteins in eleven raw and cooked model systems of meat products with 0 – 5000 ppm of powder deffated milk (PDM) and in nine raw and cooked model sy...

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Autores principales: Cellerino, Karina, Binaghi, María Julieta, Cagnasso, Carolina Elisa, Docena, Guillermo Horacio, López, Laura Beatriz
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Química
Allergens
Elisa
Dot blot
Immunoblotting
Meat products
Milk
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99853
https://ri.conicet.gov.ar/11336/36323
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-99853
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Allergens
Elisa
Dot blot
Immunoblotting
Meat products
Milk
spellingShingle Química
Allergens
Elisa
Dot blot
Immunoblotting
Meat products
Milk
Cellerino, Karina
Binaghi, María Julieta
Cagnasso, Carolina Elisa
Docena, Guillermo Horacio
López, Laura Beatriz
Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods
topic_facet Química
Allergens
Elisa
Dot blot
Immunoblotting
Meat products
Milk
description The aim of this study was to evaluate different immunochemical methods (Dot Blot, Immnoblotting and two different ELISA kits) for the detection of milk proteins in eleven raw and cooked model systems of meat products with 0 – 5000 ppm of powder deffated milk (PDM) and in nine raw and cooked model systems of meat products with 0-2000 ppm of dry whey (DW) and in eleven commercial meat products. All the samples were analysed with Dot Blot and Immunoblotting with specific polyclonal rabbit serum against milk proteins and with two ELISA kits: Veratox® Total Milk Allergen Quantitative Test from Neogen and Ridascreen® Fast Milk from R-Biopharm. ELISA methods are more sensitive for the detection of milk proteins than Dot Blot and Immunoblotting. The R-Biopharm kit was the most sensitive kit for the analysis of these samples. However Immunoblotting can be useful for the detection of milk proteins if it is suspected that they were added as ingredients or additives. Immunoblotting allows to verify the presence of caseins and / or β-lactoglobulin. In contrast, the use of an ELISA kit is more appropriate to verify a possible cross-contamination.
format Articulo
Articulo
author Cellerino, Karina
Binaghi, María Julieta
Cagnasso, Carolina Elisa
Docena, Guillermo Horacio
López, Laura Beatriz
author_facet Cellerino, Karina
Binaghi, María Julieta
Cagnasso, Carolina Elisa
Docena, Guillermo Horacio
López, Laura Beatriz
author_sort Cellerino, Karina
title Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods
title_short Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods
title_full Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods
title_fullStr Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods
title_full_unstemmed Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods
title_sort milk protein detection in raw and cooked meat products using immunochemichal methods
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/99853
https://ri.conicet.gov.ar/11336/36323
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