Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods
The aim of this study was to evaluate different immunochemical methods (Dot Blot, Immnoblotting and two different ELISA kits) for the detection of milk proteins in eleven raw and cooked model systems of meat products with 0 – 5000 ppm of powder deffated milk (PDM) and in nine raw and cooked model sy...
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Autores principales: | , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
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2014
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/99853 https://ri.conicet.gov.ar/11336/36323 |
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I19-R120-10915-99853 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Química Allergens Elisa Dot blot Immunoblotting Meat products Milk |
spellingShingle |
Química Allergens Elisa Dot blot Immunoblotting Meat products Milk Cellerino, Karina Binaghi, María Julieta Cagnasso, Carolina Elisa Docena, Guillermo Horacio López, Laura Beatriz Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods |
topic_facet |
Química Allergens Elisa Dot blot Immunoblotting Meat products Milk |
description |
The aim of this study was to evaluate different immunochemical methods (Dot Blot, Immnoblotting and two different ELISA kits) for the detection of milk proteins in eleven raw and cooked model systems of meat products with 0 – 5000 ppm of powder deffated milk (PDM) and in nine raw and cooked model systems of meat products with 0-2000 ppm of dry whey (DW) and in eleven commercial meat products. All the samples were analysed with Dot Blot and Immunoblotting with specific polyclonal rabbit serum against milk proteins and with two ELISA kits: Veratox® Total Milk Allergen Quantitative Test from Neogen and Ridascreen® Fast Milk from R-Biopharm. ELISA methods are more sensitive for the detection of milk proteins than Dot Blot and Immunoblotting. The R-Biopharm kit was the most sensitive kit for the analysis of these samples. However Immunoblotting can be useful for the detection of milk proteins if it is suspected that they were added as ingredients or additives. Immunoblotting allows to verify the presence of caseins and / or β-lactoglobulin. In contrast, the use of an ELISA kit is more appropriate to verify a possible cross-contamination. |
format |
Articulo Articulo |
author |
Cellerino, Karina Binaghi, María Julieta Cagnasso, Carolina Elisa Docena, Guillermo Horacio López, Laura Beatriz |
author_facet |
Cellerino, Karina Binaghi, María Julieta Cagnasso, Carolina Elisa Docena, Guillermo Horacio López, Laura Beatriz |
author_sort |
Cellerino, Karina |
title |
Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods |
title_short |
Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods |
title_full |
Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods |
title_fullStr |
Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods |
title_full_unstemmed |
Milk Protein Detection in Raw and Cooked Meat Products Using Immunochemichal Methods |
title_sort |
milk protein detection in raw and cooked meat products using immunochemichal methods |
publishDate |
2014 |
url |
http://sedici.unlp.edu.ar/handle/10915/99853 https://ri.conicet.gov.ar/11336/36323 |
work_keys_str_mv |
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bdutipo_str |
Repositorios |
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1764820493348634625 |