Intermediate filament protein expression and sugar moieties in normal canine placenta

In the female dog, the placenta is considered zonal, endotheliochorial and labyrinthic. The distribution of the intermediate filaments as well as the surface glycoproteins in the canine placenta are still unknown. The aim of the present study was to provide this information for further understanding...

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Autores principales: Fernández, Patricia Elena, Barbeito, Claudio Gustavo, Portiansky, Enrique Leo, Gimeno, Eduardo Juan
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99697
https://ri.conicet.gov.ar/11336/53577
http://www.hh.um.es/Abstracts/Vol_15/15_1/15_1_1.htm
Aporte de:
id I19-R120-10915-99697
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Bitch
Placenta
Lectins
Immunohistochemistry
Glycoconjugates
spellingShingle Veterinaria
Bitch
Placenta
Lectins
Immunohistochemistry
Glycoconjugates
Fernández, Patricia Elena
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
Intermediate filament protein expression and sugar moieties in normal canine placenta
topic_facet Veterinaria
Bitch
Placenta
Lectins
Immunohistochemistry
Glycoconjugates
description In the female dog, the placenta is considered zonal, endotheliochorial and labyrinthic. The distribution of the intermediate filaments as well as the surface glycoproteins in the canine placenta are still unknown. The aim of the present study was to provide this information for further understanding of pathological conditions in the bitch. Samples were obtained from normal uterine horns at the end of gestation. Tissues were routinely fixed and stained. Monoclonal antibodies against cytokeratins, vimentin and desmin were used for immunohistochemical staining. UEA-I, PNA, RCA-I, SBA, DBA, WGA and ConA were used for the lectin histochemical staining. A computer morphometrical analysis was made. Statistical analysis was then accomplished. The results showed the maximun immunohistochemical percentage for vimentin in the supra glandular connective tissue, for pancytokeratin in the spongy zone and for desmin in miometrium. SBA showed the highest staining percentage in the gland cells of the spongy zone, while ConA was the highest in the syncytiotrophoblastic cells and gland cells of the deep glandular zone. The results obtained indicate that the lectin binding pattern is partially different from other animal species. On the contrary, the intermediate filament data coincide with similar observations from other mammals.
format Articulo
Articulo
author Fernández, Patricia Elena
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author_facet Fernández, Patricia Elena
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author_sort Fernández, Patricia Elena
title Intermediate filament protein expression and sugar moieties in normal canine placenta
title_short Intermediate filament protein expression and sugar moieties in normal canine placenta
title_full Intermediate filament protein expression and sugar moieties in normal canine placenta
title_fullStr Intermediate filament protein expression and sugar moieties in normal canine placenta
title_full_unstemmed Intermediate filament protein expression and sugar moieties in normal canine placenta
title_sort intermediate filament protein expression and sugar moieties in normal canine placenta
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/99697
https://ri.conicet.gov.ar/11336/53577
http://www.hh.um.es/Abstracts/Vol_15/15_1/15_1_1.htm
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