Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process

Discoidal high-density lipoproteins (D-HDL) are critical intermediates in reverse cholesterol transport. Most of the present knowledge of D-HDL is based on studies with reconstituted lipoprotein complexes of apolipoprotein A-I (apoA-I) obtained by cholate dialysis (CD). D-HDL can also be generated b...

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Autores principales:	Cuellar Rodríguez, Luz Ángela, Prieto, Eduardo Daniel, Cabaleiro, Laura Virginia, Garda, Horacio Alberto
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2013
Materias:	Biología Apolipoproteins Fluorescence resonance energy transfer (fret) Lipoprotein structure Site directed mutagenesis Single tryptophan mutants Cysteine mutants
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/99669 https://ri.conicet.gov.ar/11336/4766
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-99669
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Apolipoproteins Fluorescence resonance energy transfer (fret) Lipoprotein structure Site directed mutagenesis Single tryptophan mutants Cysteine mutants
spellingShingle	Biología Apolipoproteins Fluorescence resonance energy transfer (fret) Lipoprotein structure Site directed mutagenesis Single tryptophan mutants Cysteine mutants Cuellar Rodríguez, Luz Ángela Prieto, Eduardo Daniel Cabaleiro, Laura Virginia Garda, Horacio Alberto Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
topic_facet	Biología Apolipoproteins Fluorescence resonance energy transfer (fret) Lipoprotein structure Site directed mutagenesis Single tryptophan mutants Cysteine mutants
description	Discoidal high-density lipoproteins (D-HDL) are critical intermediates in reverse cholesterol transport. Most of the present knowledge of D-HDL is based on studies with reconstituted lipoprotein complexes of apolipoprotein A-I (apoA-I) obtained by cholate dialysis (CD). D-HDL can also be generated by the direct microsolubilization (DM) of phospholipid vesicles at the gel/fluid phase transition temperature, a process mechanistically similar to the "in vivo" apoAI lipidation via ABCA1. We compared the apoA-I configuration in D-HDL reconstituted with dimyristoylphosphatidylcholine by both procedures using fluorescence resonance energy transfer measurements with apoA-I tryptophan mutants and fluorescently labeled cysteine mutants. Results indicate that apoA-I configuration in D-HDL depends on the reconstitution process and are consistent with a "double belt" molecular arrangement with different helix registry. As reported by others, a configuration with juxtaposition of helices 5 of each apoAI monomer (5/5 registry) predominates in D-HDL obtained by CD. However, a configuration with helix 5 of one monomer juxtaposed with helix 2 of the other (5/2 registry) would predominate in D-HDL generated by DM. Moreover, we also show that the kinetics of cholesterol efflux from macrophage cultures depends on the reconstitution process, suggesting that apoAI configuration is important for this HDL function.
format	Articulo Preprint
author	Cuellar Rodríguez, Luz Ángela Prieto, Eduardo Daniel Cabaleiro, Laura Virginia Garda, Horacio Alberto
author_facet	Cuellar Rodríguez, Luz Ángela Prieto, Eduardo Daniel Cabaleiro, Laura Virginia Garda, Horacio Alberto
author_sort	Cuellar Rodríguez, Luz Ángela
title	Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
title_short	Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
title_full	Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
title_fullStr	Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
title_full_unstemmed	Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
title_sort	apolipoprotein a-i configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/99669 https://ri.conicet.gov.ar/11336/4766
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bdutipo_str	Repositorios
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Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process

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