Behavior of ligand binding assays with crowded surfaces: molecular model of antigen capture by antibody-conjugated nanoparticles

Ligand-receptor binding is of utmost importance in several biologically related disciplines. Ligand binding assays (LBA) use the high specificity and high affinity of ligands to detect, target or measure a specific receptors. One particular example of ligand binding assays are Antibody conjugated Na...

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Autores principales: Malaspina, David César, Longo, Gabriel Sebastián, Szleifer, Igal
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Nanoparticles
Free energy
Chemical equilibrium
Binding analysis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99504
https://ri.conicet.gov.ar/11336/63956
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185518
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-99504
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Nanoparticles
Free energy
Chemical equilibrium
Binding analysis
spellingShingle Química
Nanoparticles
Free energy
Chemical equilibrium
Binding analysis
Malaspina, David César
Longo, Gabriel Sebastián
Szleifer, Igal
Behavior of ligand binding assays with crowded surfaces: molecular model of antigen capture by antibody-conjugated nanoparticles
topic_facet Química
Nanoparticles
Free energy
Chemical equilibrium
Binding analysis
description Ligand-receptor binding is of utmost importance in several biologically related disciplines. Ligand binding assays (LBA) use the high specificity and high affinity of ligands to detect, target or measure a specific receptors. One particular example of ligand binding assays are Antibody conjugated Nanoparticles (AcNPs), edge-cutting technologies that are present in several novel biomedical approaches for imaging, detection and treatment of diseases. However, the nano-confinement in AcNPs and LBA nanostructures introduces extra complexity in the analysis of ligand-receptor equilibriums. Because antibodies are large voluminous ligands, the effective affinity in AcNPs is often determined by antibody orientation and surface coverage. Moreover, antibodies have two binding sites introducing an extra ligand-receptor binding equilibrium. As consequence of all this, experimental or theoretical studies providing a guidelines for the prediction of the binding behavior in AcNPs are scarce. In this work, we present a set of theoretical calculations to shed light into the complex binding behavior of AcNPs and its implications in biomedical applications. To investigate the ligand-receptor binding on AcNPs, we have used a molecular theory that predicts the probability of different molecular conformations of the system depending on the local environment. We have considered two different pathways for designing these devices: covalently conjugated antibodies and streptavidin-biotin conjugated antibodies. We also explore the effects of surface coverage, bulk concentrations, nanoparticle size and antibody-antigen affinity. Overall, this work offers a series of theoretical predictions that can be used as a guide in the design of antibody conjugated nanoparticles for different applications.
format Articulo
Articulo
author Malaspina, David César
Longo, Gabriel Sebastián
Szleifer, Igal
author_facet Malaspina, David César
Longo, Gabriel Sebastián
Szleifer, Igal
author_sort Malaspina, David César
title Behavior of ligand binding assays with crowded surfaces: molecular model of antigen capture by antibody-conjugated nanoparticles
title_short Behavior of ligand binding assays with crowded surfaces: molecular model of antigen capture by antibody-conjugated nanoparticles
title_full Behavior of ligand binding assays with crowded surfaces: molecular model of antigen capture by antibody-conjugated nanoparticles
title_fullStr Behavior of ligand binding assays with crowded surfaces: molecular model of antigen capture by antibody-conjugated nanoparticles
title_full_unstemmed Behavior of ligand binding assays with crowded surfaces: molecular model of antigen capture by antibody-conjugated nanoparticles
title_sort behavior of ligand binding assays with crowded surfaces: molecular model of antigen capture by antibody-conjugated nanoparticles
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/99504
https://ri.conicet.gov.ar/11336/63956
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185518
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