Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization

Rhizobia are α‐ and ß‐proteobacteria that associate with legumes in symbiosis to fix atmospheric nitrogen. The chemical communication between roots and rhizobia begins in the rhizosphere. Using signature‐tagged‐Tn5 mutagenesis (STM) we performed a genome‐wide screening for Ensifer meliloti genes tha...

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Detalles Bibliográficos
Autores principales:	Salas, María Eugenia, Lozano, Mauricio Javier, López, José Luis, Draghi, Walter Omar, Serrania, Javier, Torres Tejerizo, Gonzalo Arturo, Albicoro, Francisco Javier, Nilsson, Juliet Fernanda, Pistorio, Mariano, Del Papa, María Florencia, Parisi, Gustavo Daniel, Becker, Anke, Lagares, Antonio
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2017
Materias:	Biología Rhizobia Rhizosphere STM Specificity
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/99489 https://ri.conicet.gov.ar/11336/48016 https://onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.13820
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-99489
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Rhizobia Rhizosphere STM Specificity
spellingShingle	Biología Rhizobia Rhizosphere STM Specificity Salas, María Eugenia Lozano, Mauricio Javier López, José Luis Draghi, Walter Omar Serrania, Javier Torres Tejerizo, Gonzalo Arturo Albicoro, Francisco Javier Nilsson, Juliet Fernanda Pistorio, Mariano Del Papa, María Florencia Parisi, Gustavo Daniel Becker, Anke Lagares, Antonio Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization
topic_facet	Biología Rhizobia Rhizosphere STM Specificity
description	Rhizobia are α‐ and ß‐proteobacteria that associate with legumes in symbiosis to fix atmospheric nitrogen. The chemical communication between roots and rhizobia begins in the rhizosphere. Using signature‐tagged‐Tn5 mutagenesis (STM) we performed a genome‐wide screening for Ensifer meliloti genes that participate in colonizing the rhizospheres of alfalfa and other legumes. The analysis of ca. 6,000 mutants indicated that genes relevant for rhizosphere colonization account for nearly 2% of the rhizobial genome and that most (ca. 80%) are chromosomally located, pointing to the relevance and ancestral origin of the bacterial ability to colonize plant roots. The identified genes were related to metabolic functions, transcription, signal transduction, and motility/chemotaxis among other categories; with several ORFs of yet‐unknown function. Most remarkably, we identified a subset of genes that impacted more severely the colonization of the roots of alfalfa than of pea. Further analyses using other plant species revealed that such early differential phenotype could be extended to other members of the Trifoliae tribe (Trigonella, Trifolium), but not the Fabeae and Phaseoleae tribes. The results suggest that consolidation of E. meliloti into its current symbiotic state should have occurred in a rhizobacterium that had already been adapted to rhizospheres of the Trifoliae tribe.
format	Articulo Preprint
author	Salas, María Eugenia Lozano, Mauricio Javier López, José Luis Draghi, Walter Omar Serrania, Javier Torres Tejerizo, Gonzalo Arturo Albicoro, Francisco Javier Nilsson, Juliet Fernanda Pistorio, Mariano Del Papa, María Florencia Parisi, Gustavo Daniel Becker, Anke Lagares, Antonio
author_facet	Salas, María Eugenia Lozano, Mauricio Javier López, José Luis Draghi, Walter Omar Serrania, Javier Torres Tejerizo, Gonzalo Arturo Albicoro, Francisco Javier Nilsson, Juliet Fernanda Pistorio, Mariano Del Papa, María Florencia Parisi, Gustavo Daniel Becker, Anke Lagares, Antonio
author_sort	Salas, María Eugenia
title	Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization
title_short	Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization
title_full	Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization
title_fullStr	Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization
title_full_unstemmed	Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization
title_sort	specificity traits consistent with legume-rhizobia coevolution displayed by ensifer meliloti rhizosphere colonization
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/99489 https://ri.conicet.gov.ar/11336/48016 https://onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.13820
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Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization

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