Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling

Several methods such as PCR-RFLP, OLA, DNA sequencing, PCR-SSCP and ARMS-PCR have been developed to detect the allelic variation at substitutions K232A of DGAT1, GH6.1 of GH, F279Y of GHR, R4C of LEP, I74V of FABP4, and at the transition in the TG 5´ leader sequence. Most of these methods are manual...

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Autores principales:	Ripoli, María Verónica, Rogberg Muñoz, Andrés, Lirón, Juan Pedro, Giovambattista, Guillermo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2013
Materias:	Ciencias Veterinarias Bovine molecular markers Polymorphism Pyrosequencing Marbling Marcadores moleculares bovinos Polimorfismos Pirosecuenciación Marmoleo
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/99474 https://ri.conicet.gov.ar/11336/11612
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-99474
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Veterinarias Bovine molecular markers Polymorphism Pyrosequencing Marbling Marcadores moleculares bovinos Polimorfismos Pirosecuenciación Marmoleo
spellingShingle	Ciencias Veterinarias Bovine molecular markers Polymorphism Pyrosequencing Marbling Marcadores moleculares bovinos Polimorfismos Pirosecuenciación Marmoleo Ripoli, María Verónica Rogberg Muñoz, Andrés Lirón, Juan Pedro Giovambattista, Guillermo Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
topic_facet	Ciencias Veterinarias Bovine molecular markers Polymorphism Pyrosequencing Marbling Marcadores moleculares bovinos Polimorfismos Pirosecuenciación Marmoleo
description	Several methods such as PCR-RFLP, OLA, DNA sequencing, PCR-SSCP and ARMS-PCR have been developed to detect the allelic variation at substitutions K232A of DGAT1, GH6.1 of GH, F279Y of GHR, R4C of LEP, I74V of FABP4, and at the transition in the TG 5´ leader sequence. Most of these methods are manual processes and therefore increase the time spent on the assay and limit the number of animals analyzed. Herein, we describe the development of pyrosequencing-based methods for the bovine DGAT1, GH, GHR, LEP, FABP4 and TG genes, whose polymorphisms have been associated with variation in carcass composition. This method was validated by analyzing DNA samples belonging to the Aberdeen Angus and Hereford breeds previously typed by PCR-SSCP, PCR-RFLP and/or DNA sequencing. The results obtained showed that, after sequencing or whole-genome association studies (discovery step), the pyrosequencing-based technique seems to be useful to validate (validation step) a particular single nucleotide polymorphism (SNP) in a candidate gene in a previously mapped region in independent populations (with different genotypes and/or production systems). We conclude that pyrosequencing may be useful in high-throughput SNP genotyping of candidate genes in breeds of cattle and other animal species, making it a fast and interesting screening method for population or association studies.
format	Articulo Articulo
author	Ripoli, María Verónica Rogberg Muñoz, Andrés Lirón, Juan Pedro Giovambattista, Guillermo
author_facet	Ripoli, María Verónica Rogberg Muñoz, Andrés Lirón, Juan Pedro Giovambattista, Guillermo
author_sort	Ripoli, María Verónica
title	Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
title_short	Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
title_full	Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
title_fullStr	Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
title_full_unstemmed	Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
title_sort	development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/99474 https://ri.conicet.gov.ar/11336/11612
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bdutipo_str	Repositorios
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Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling

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