Microplate assay for the determination of carboxypeptidase A inhibitory activity in Andean potatoes

Metallocarboxypeptidases (MCPs) are zinc-dependent exopeptidases that catalyze the hydrolysis of C-terminal amide bonds in proteins and peptides. MCPs are involved in a wide range of physiological processes and have recently emerged as relevant drug targets in biomedicine (Arolas et. al., 2007). In...

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Autores principales: Tellechea, Mariana Edith, Garcia Pardo, Javier, Cotabarren, Juliana, Lufrano, Daniela, Bakás, Laura Susana, Avilés, Francesc Xavier, Obregon, Walter David, Lorenzo, Julia, Tanco, Sebastian
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Bioquímica
Metallocarboxypeptidase
Inhibitor
Microplate assay
Carboxypeptidase A
Inhibitory activity
Potatoes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99436
https://ri.conicet.gov.ar/11336/55082
https://bio-protocol.org/e2032
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-99436
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Metallocarboxypeptidase
Inhibitor
Microplate assay
Carboxypeptidase A
Inhibitory activity
Potatoes
spellingShingle Bioquímica
Metallocarboxypeptidase
Inhibitor
Microplate assay
Carboxypeptidase A
Inhibitory activity
Potatoes
Tellechea, Mariana Edith
Garcia Pardo, Javier
Cotabarren, Juliana
Lufrano, Daniela
Bakás, Laura Susana
Avilés, Francesc Xavier
Obregon, Walter David
Lorenzo, Julia
Tanco, Sebastian
Microplate assay for the determination of carboxypeptidase A inhibitory activity in Andean potatoes
topic_facet Bioquímica
Metallocarboxypeptidase
Inhibitor
Microplate assay
Carboxypeptidase A
Inhibitory activity
Potatoes
description Metallocarboxypeptidases (MCPs) are zinc-dependent exopeptidases that catalyze the hydrolysis of C-terminal amide bonds in proteins and peptides. MCPs are involved in a wide range of physiological processes and have recently emerged as relevant drug targets in biomedicine (Arolas et. al., 2007). In higher plants, small proteinaceous protease inhibitors are wound-induced molecules produced as a part of its defense system against insect attack (Graham et. al., 1981; Villanueva et. al. 1998). Among such inhibitors, only two are specific for MCPs, i.e. the potato carboxypeptidase inhibitor (PCI) and its close homologue found in tomato plants. In humans, MCP action is exquisitely regulated and dysregulation of their function might lead to disease or even to cell death (Arolas et. al., 2007). In this context, the discovery and characterization of new MCPs inhibitors constitute a valuable approach for the development of new therapeutic strategies. Over the last few decades, the presence of MCPs inhibitors in Solanaceae has been extensively reported, revealing potato (Solanum tuberosum) as one of the most important sources of MCPs inhibitors (Hass et. al.,1979; Lufrano et. al., 2015). In this context, potatos are native from the Andean region of South America, where thousands of different potato varieties coexist, constituting a natural reservoir for the discovery of novel MCP inhibitors (Figure 1). In this protocol, we describe an optimized, simple and accessible microplate method for the measure of the specific and dose-response carboxypeptidase A inhibitory activities present in Andean potatoes tubers.
format Articulo
Articulo
author Tellechea, Mariana Edith
Garcia Pardo, Javier
Cotabarren, Juliana
Lufrano, Daniela
Bakás, Laura Susana
Avilés, Francesc Xavier
Obregon, Walter David
Lorenzo, Julia
Tanco, Sebastian
author_facet Tellechea, Mariana Edith
Garcia Pardo, Javier
Cotabarren, Juliana
Lufrano, Daniela
Bakás, Laura Susana
Avilés, Francesc Xavier
Obregon, Walter David
Lorenzo, Julia
Tanco, Sebastian
author_sort Tellechea, Mariana Edith
title Microplate assay for the determination of carboxypeptidase A inhibitory activity in Andean potatoes
title_short Microplate assay for the determination of carboxypeptidase A inhibitory activity in Andean potatoes
title_full Microplate assay for the determination of carboxypeptidase A inhibitory activity in Andean potatoes
title_fullStr Microplate assay for the determination of carboxypeptidase A inhibitory activity in Andean potatoes
title_full_unstemmed Microplate assay for the determination of carboxypeptidase A inhibitory activity in Andean potatoes
title_sort microplate assay for the determination of carboxypeptidase a inhibitory activity in andean potatoes
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/99436
https://ri.conicet.gov.ar/11336/55082
https://bio-protocol.org/e2032
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