Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells

Equine Arteritis Virus (EAV) has been shown to induce apoptosis in vitro but the induction of this mechanism has not been previously associated with any viral gene product. In this work, we found a cytotoxicity effect of the EAV gP5 protein on baculovirus-insect cells and a low yield of protein reco...

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Autores principales: Metz, Germán Ernesto, Serena, María Soledad, Abeyá, María Mercedes, Dulbecco, Andrea Belén, Massone, Adriana Raquel, Díaz, Silvina, Echeverría, María Gabriela
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Veterinarias
Equine arteritis virus
Apoptosis
gP5 protein
Insect cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98898
https://ri.conicet.gov.ar/11336/11504
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98898
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Equine arteritis virus
Apoptosis
gP5 protein
Insect cells
spellingShingle Ciencias Veterinarias
Equine arteritis virus
Apoptosis
gP5 protein
Insect cells
Metz, Germán Ernesto
Serena, María Soledad
Abeyá, María Mercedes
Dulbecco, Andrea Belén
Massone, Adriana Raquel
Díaz, Silvina
Echeverría, María Gabriela
Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells
topic_facet Ciencias Veterinarias
Equine arteritis virus
Apoptosis
gP5 protein
Insect cells
description Equine Arteritis Virus (EAV) has been shown to induce apoptosis in vitro but the induction of this mechanism has not been previously associated with any viral gene product. In this work, we found a cytotoxicity effect of the EAV gP5 protein on baculovirus-insect cells and a low yield of protein recovery. Besides, different morphological features by electron transmission microscopy, DNA fragmentation in agarose gel, TUNEL analysis and caspase 3 activity were found. All these findings indicate that the EAV gP5 protein induces apoptosis in insect cells.
format Articulo
Preprint
author Metz, Germán Ernesto
Serena, María Soledad
Abeyá, María Mercedes
Dulbecco, Andrea Belén
Massone, Adriana Raquel
Díaz, Silvina
Echeverría, María Gabriela
author_facet Metz, Germán Ernesto
Serena, María Soledad
Abeyá, María Mercedes
Dulbecco, Andrea Belén
Massone, Adriana Raquel
Díaz, Silvina
Echeverría, María Gabriela
author_sort Metz, Germán Ernesto
title Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells
title_short Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells
title_full Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells
title_fullStr Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells
title_full_unstemmed Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells
title_sort equine arteritis virus gp5 protein induces apoptosis in cultured insect cells
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/98898
https://ri.conicet.gov.ar/11336/11504
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