Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes

BACKGROUND: The koji mold Aspergillus sojae, an industrially important microorganism, can produce high levels of pectinases utilizing agro-industrial wastes. This study introduces apricot and peach pomace, two agro-industrial wastes barely considered as raw material for the generation of value-added...

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Autores principales: Fratebianchi de la Parra, Dante, Crespo, Juan Manuel, Tari, Canan, Cavalitto, Sebastián Fernando
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Agro-industrial wastes
Batch fermentation
Oxygen limitation
Response surface design
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98827
https://ri.conicet.gov.ar/11336/87824
https://onlinelibrary.wiley.com/doi/full/10.1002/jctb.5006
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98827
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Agro-industrial wastes
Batch fermentation
Oxygen limitation
Response surface design
spellingShingle Química
Agro-industrial wastes
Batch fermentation
Oxygen limitation
Response surface design
Fratebianchi de la Parra, Dante
Crespo, Juan Manuel
Tari, Canan
Cavalitto, Sebastián Fernando
Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes
topic_facet Química
Agro-industrial wastes
Batch fermentation
Oxygen limitation
Response surface design
description BACKGROUND: The koji mold Aspergillus sojae, an industrially important microorganism, can produce high levels of pectinases utilizing agro-industrial wastes. This study introduces apricot and peach pomace, two agro-industrial wastes barely considered as raw material for the generation of value-added products, and focuses on its utilization together with orange peel for polygalacturonase production in submerged cultures using A. sojae. RESULTS: A Doehlert response surface methodology design conducted in shake flasks and applied individually with these three by-products led to 60–80 U mL−1 polygalacturonase activity. In bioreactor studies performed with a mixture of apricot pomace and orange peel, by fixing stirrer speed to 600 rpm and cascading airflow to the dissolved oxygen tension up to 1.7 vvm, oxygen limitation problems were overcome and polygalacturonase activity values of 380 U mL−1 were achieved. CONCLUSION: A simple and efficient strategy to minimize oxygen limitation with the lowest possible shear stress is provided for stirred-tank bioreactors working with highly viscous broths, so as to ultimately enhance microbial enzyme production. The polygalacturonase activity yields obtained in our study are among the highest reported in the literature.
format Articulo
Preprint
author Fratebianchi de la Parra, Dante
Crespo, Juan Manuel
Tari, Canan
Cavalitto, Sebastián Fernando
author_facet Fratebianchi de la Parra, Dante
Crespo, Juan Manuel
Tari, Canan
Cavalitto, Sebastián Fernando
author_sort Fratebianchi de la Parra, Dante
title Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes
title_short Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes
title_full Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes
title_fullStr Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes
title_full_unstemmed Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes
title_sort control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by aspergillus sojae using agro-industrial wastes
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/98827
https://ri.conicet.gov.ar/11336/87824
https://onlinelibrary.wiley.com/doi/full/10.1002/jctb.5006
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