SERCA is critical to control the Bowditch effect in the heart

The Bowditch effect or staircase phenomenon is the increment or reduction of contractile force when heart rate increases, defined as either a positive or negative staircase. The healthy and failing human heart both show positive or negative staircase, respectively, but the causes of these distinct c...

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Autores principales: Balcazar, Dario Emmanuel, Regge, María Victoria, Santalla, Manuela, Meyer, Heiko, Paululat, Achim, Mattiazzi, Alicia Ramona, Ferrero, Paola Viviana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Médicas
Drosophila
Serca
Bowditch effect
Heart pathophysiology
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98353
https://ri.conicet.gov.ar/11336/96370
http://www.nature.com/articles/s41598-018-30638-9
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98353
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Drosophila
Serca
Bowditch effect
Heart pathophysiology
spellingShingle Ciencias Médicas
Drosophila
Serca
Bowditch effect
Heart pathophysiology
Balcazar, Dario Emmanuel
Regge, María Victoria
Santalla, Manuela
Meyer, Heiko
Paululat, Achim
Mattiazzi, Alicia Ramona
Ferrero, Paola Viviana
SERCA is critical to control the Bowditch effect in the heart
topic_facet Ciencias Médicas
Drosophila
Serca
Bowditch effect
Heart pathophysiology
description The Bowditch effect or staircase phenomenon is the increment or reduction of contractile force when heart rate increases, defined as either a positive or negative staircase. The healthy and failing human heart both show positive or negative staircase, respectively, but the causes of these distinct cardiac responses are unclear. Different experimental approaches indicate that while the level of Ca2+ in the sarcoplasmic reticulum is critical, the molecular mechanisms are unclear. Here, we demonstrate that Drosophila melanogaster shows a negative staircase which is associated to a slight but significant frequency-dependent acceleration of relaxation (FDAR) at the highest stimulation frequencies tested. We further showed that the type of staircase is oppositely modified by two distinct SERCA mutations. The dominant conditional mutation SERCAA617T induced positive staircase and arrhythmia, while SERCAE442K accentuated the negative staircase of wild type. At the stimulation frequencies tested, no significant FDAR could be appreciated in mutant flies. The present results provide evidence that two individual mutations directly modify the type of staircase occurring within the heart and suggest an important role of SERCA in regulating the Bowditch effect.
format Articulo
Articulo
author Balcazar, Dario Emmanuel
Regge, María Victoria
Santalla, Manuela
Meyer, Heiko
Paululat, Achim
Mattiazzi, Alicia Ramona
Ferrero, Paola Viviana
author_facet Balcazar, Dario Emmanuel
Regge, María Victoria
Santalla, Manuela
Meyer, Heiko
Paululat, Achim
Mattiazzi, Alicia Ramona
Ferrero, Paola Viviana
author_sort Balcazar, Dario Emmanuel
title SERCA is critical to control the Bowditch effect in the heart
title_short SERCA is critical to control the Bowditch effect in the heart
title_full SERCA is critical to control the Bowditch effect in the heart
title_fullStr SERCA is critical to control the Bowditch effect in the heart
title_full_unstemmed SERCA is critical to control the Bowditch effect in the heart
title_sort serca is critical to control the bowditch effect in the heart
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/98353
https://ri.conicet.gov.ar/11336/96370
http://www.nature.com/articles/s41598-018-30638-9
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bdutipo_str Repositorios
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