Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations

The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 μg/ml (Zn1); 1.1 μg/ml (Zn2) and 1.5 μg/ml (Zn3)] to the culture m...

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Detalles Bibliográficos
Autores principales: Anchordoquy, Juan Mateo, Picco, Sebastián Julio, Seoane, Analía Isabel, Anchordoquy, Juan Patricio, Ponzinibbio, María Virginia, Mattioli, Guillermo Alberto, Peral García, Pilar, Furnus, Cecilia Cristina
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2011
Materias:
Biología
Annexin
Apoptosis
Comet assay
DNA damage
Cumulus cells
Zinc
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98333
https://ri.conicet.gov.ar/11336/93853
https://onlinelibrary.wiley.com/doi/abs/10.1042/CBI20100507
Aporte de:
Aportado por : SEDICI (UNLP) de Universidad Nacional de La Plata .
id I19-R120-10915-98333
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spelling I19-R120-10915-983332020-10-27T04:07:42Z http://sedici.unlp.edu.ar/handle/10915/98333 https://ri.conicet.gov.ar/11336/93853 https://onlinelibrary.wiley.com/doi/abs/10.1042/CBI20100507 issn:1065-6995 Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations Anchordoquy, Juan Mateo Picco, Sebastián Julio Seoane, Analía Isabel Anchordoquy, Juan Patricio Ponzinibbio, María Virginia Mattioli, Guillermo Alberto Peral García, Pilar Furnus, Cecilia Cristina 2011-05 2020-06-16T18:52:04Z en Biología Annexin Apoptosis Comet assay DNA damage Cumulus cells Zinc The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 μg/ml (Zn1); 1.1 μg/ml (Zn2) and 1.5 μg/ml (Zn3)] to the culture medium was evaluated by comet assay. In addition, early apoptosis was analysed by annexin staining assay. CCs treated with Zn showed a significant decrease in the DNA damage in a dosedependent manner. Comet assay analysed for TM (tail moment) was significantly higher in cells cultured without Zn (control, P<0.01) with respect to cells treated with Zn (control: 5.24±16.05; Zn1: 1.13±5.31; Zn2: 0.10±0.36; Zn3: 0.017±0.06). All treatments were statistically different from the control (P50.014 for Zn1; P,0.01 for Zn2 and Zn3). The frequency of apoptotic cells was higher in the control group (control: 0.142±0.07; Zn1: 0.109±0.0328; Zn2:0.102±0.013; Zn3: 0.0577±0.019). Statistical differences were found between control and Zn1 (P=0.0308), control and Zn2 (P=0.0077), control and Zn3 (P<0.0001), Zn1 and Zn3 (P<0.001) and Zn2 and Zn3 (P=0.0004). No differences were found between Zn1 and Zn2. In conclusion, low Zn concentrations increase DNA damage and apoptosis in CCs cultured in vitro. However, adequate Zn concentrations 'protect' the integrity of DNA molecule and diminish the percentage of apoptotic CC. Instituto de Genética Veterinaria Articulo Preprint http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 593-597
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Annexin
Apoptosis
Comet assay
DNA damage
Cumulus cells
Zinc
spellingShingle Biología
Annexin
Apoptosis
Comet assay
DNA damage
Cumulus cells
Zinc
Anchordoquy, Juan Mateo
Picco, Sebastián Julio
Seoane, Analía Isabel
Anchordoquy, Juan Patricio
Ponzinibbio, María Virginia
Mattioli, Guillermo Alberto
Peral García, Pilar
Furnus, Cecilia Cristina
Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations
topic_facet Biología
Annexin
Apoptosis
Comet assay
DNA damage
Cumulus cells
Zinc
description The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 μg/ml (Zn1); 1.1 μg/ml (Zn2) and 1.5 μg/ml (Zn3)] to the culture medium was evaluated by comet assay. In addition, early apoptosis was analysed by annexin staining assay. CCs treated with Zn showed a significant decrease in the DNA damage in a dosedependent manner. Comet assay analysed for TM (tail moment) was significantly higher in cells cultured without Zn (control, P<0.01) with respect to cells treated with Zn (control: 5.24±16.05; Zn1: 1.13±5.31; Zn2: 0.10±0.36; Zn3: 0.017±0.06). All treatments were statistically different from the control (P50.014 for Zn1; P,0.01 for Zn2 and Zn3). The frequency of apoptotic cells was higher in the control group (control: 0.142±0.07; Zn1: 0.109±0.0328; Zn2:0.102±0.013; Zn3: 0.0577±0.019). Statistical differences were found between control and Zn1 (P=0.0308), control and Zn2 (P=0.0077), control and Zn3 (P<0.0001), Zn1 and Zn3 (P<0.001) and Zn2 and Zn3 (P=0.0004). No differences were found between Zn1 and Zn2. In conclusion, low Zn concentrations increase DNA damage and apoptosis in CCs cultured in vitro. However, adequate Zn concentrations 'protect' the integrity of DNA molecule and diminish the percentage of apoptotic CC.
format Articulo
Preprint
author Anchordoquy, Juan Mateo
Picco, Sebastián Julio
Seoane, Analía Isabel
Anchordoquy, Juan Patricio
Ponzinibbio, María Virginia
Mattioli, Guillermo Alberto
Peral García, Pilar
Furnus, Cecilia Cristina
author_facet Anchordoquy, Juan Mateo
Picco, Sebastián Julio
Seoane, Analía Isabel
Anchordoquy, Juan Patricio
Ponzinibbio, María Virginia
Mattioli, Guillermo Alberto
Peral García, Pilar
Furnus, Cecilia Cristina
author_sort Anchordoquy, Juan Mateo
title Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations
title_short Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations
title_full Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations
title_fullStr Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations
title_full_unstemmed Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations
title_sort analysis of apoptosis and dna damage in bovine cumulus cells after exposure in vitro to different zinc concentrations
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/98333
https://ri.conicet.gov.ar/11336/93853
https://onlinelibrary.wiley.com/doi/abs/10.1042/CBI20100507
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