Morphological and Molecular Characteristics of <i>Sarcocystis aucheniae</i> Isolated from Meat of Guanaco (<i>Lama guanicoe</i>)

<b>Background</b>: Sarcocystosis in South American camelids (SAC) is an important parasitic disease which results in economical loss due to carcass condemnation. Meat products from camelids are significant source of animal protein in several American countries. <i>Sarcocystis</i...

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Autores principales: Regensburger, Cristian Daniel, Gos, María Laura, Ctibor, J., Moré, Gastón Andrés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Veterinaria
Sarcocystis
Polymerase chain reaction
Sequence analysis
DNA
Microscopy
Electron
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98179
https://ri.conicet.gov.ar/11336/55022
http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1-115&slc_lang=fa&sid=1
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98179
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Sarcocystis
Polymerase chain reaction
Sequence analysis
DNA
Microscopy
Electron
spellingShingle Veterinaria
Sarcocystis
Polymerase chain reaction
Sequence analysis
DNA
Microscopy
Electron
Regensburger, Cristian Daniel
Gos, María Laura
Ctibor, J.
Moré, Gastón Andrés
Morphological and Molecular Characteristics of <i>Sarcocystis aucheniae</i> Isolated from Meat of Guanaco (<i>Lama guanicoe</i>)
topic_facet Veterinaria
Sarcocystis
Polymerase chain reaction
Sequence analysis
DNA
Microscopy
Electron
description <b>Background</b>: Sarcocystosis in South American camelids (SAC) is an important parasitic disease which results in economical loss due to carcass condemnation. Meat products from camelids are significant source of animal protein in several American countries. <i>Sarcocystis</i> spp. producing macroscopical cysts in these animals have been nominated as <i>S. aucheniae</i>, <i>S. tilopodi</i>, and <i>S. guanicoecanis</i>. The aim of the present study was to characterize morphologically and molecularly <i>Sarcocystis</i> spp. producing macroscopical cysts in guanacos (<i>Lama guanicoe</i>). <b>Methods</b>: Samples of macroscopical cysts were dissected from guanaco muscles slaughtered in Santa Cruz province, Argentina. The samples were processed by transmission electron microscopy, polymerase chain reaction (PCR), cloning and sequencing studies. <b>Results</b>: Cyst wall villar protrusions were irregular with a “tree” or “cauliflower-like” shape which measured 3-4.5 µm height by 2.5-3.5 µm width. A consensus <i>18S rRNA</i> full gene sequence of 1875 bp was obtained and showed the highest identity (≥ 99 %) with sequences of <i>S. aucheniae</i> reported in GenBank. The ultra structure of the cyst wall from macroscopical cysts and 18S rRNA gene obtained from guanacos were identical and consistent with the descriptions of <i>S. aucheniae</i>. <b>Conclusion</b>: This study confirms that guanacos are infected by the same <i>Sarcocystis</i> spp. affecting other SAC, named <i>S. aucheniae</i>, and reinforce the suggestion that nomination of new species should contain morphological and molecular data to reach validity. In future, this data could be useful to obtain proper identifications in post-mortem inspection and helpful to improve meat safety.
format Articulo
Articulo
author Regensburger, Cristian Daniel
Gos, María Laura
Ctibor, J.
Moré, Gastón Andrés
author_facet Regensburger, Cristian Daniel
Gos, María Laura
Ctibor, J.
Moré, Gastón Andrés
author_sort Regensburger, Cristian Daniel
title Morphological and Molecular Characteristics of <i>Sarcocystis aucheniae</i> Isolated from Meat of Guanaco (<i>Lama guanicoe</i>)
title_short Morphological and Molecular Characteristics of <i>Sarcocystis aucheniae</i> Isolated from Meat of Guanaco (<i>Lama guanicoe</i>)
title_full Morphological and Molecular Characteristics of <i>Sarcocystis aucheniae</i> Isolated from Meat of Guanaco (<i>Lama guanicoe</i>)
title_fullStr Morphological and Molecular Characteristics of <i>Sarcocystis aucheniae</i> Isolated from Meat of Guanaco (<i>Lama guanicoe</i>)
title_full_unstemmed Morphological and Molecular Characteristics of <i>Sarcocystis aucheniae</i> Isolated from Meat of Guanaco (<i>Lama guanicoe</i>)
title_sort morphological and molecular characteristics of <i>sarcocystis aucheniae</i> isolated from meat of guanaco (<i>lama guanicoe</i>)
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/98179
https://ri.conicet.gov.ar/11336/55022
http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1-115&slc_lang=fa&sid=1
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