Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles

The aims of this work were to: (i) microscopically analyse the pre- and post-Columbian archaeological textiles using Scanning Electron Microscopy with Energy Dispersive X-Ray Analysis (SEM-EDX); (ii) microbiologically analyse the archaeological textiles (from the Southern Andean Area, La Plata Museu...

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Detalles Bibliográficos
Autores principales: Pietrzak, Katarzyna, Otlewska, Anna, Puchalski, Michal, Gutarowska, Beata, Guiamet, Patricia Sandra
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Física
Archaeological textiles
Pseudomonas
Protection
Silver nanoparticles
Biofilm
SEM-EDX
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98113
https://ri.conicet.gov.ar/11336/64092
https://www.udspub.com/ajj/public/index.php/aeb/article/view/145/118
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98113
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Física
Archaeological textiles
Pseudomonas
Protection
Silver nanoparticles
Biofilm
SEM-EDX
spellingShingle Química
Física
Archaeological textiles
Pseudomonas
Protection
Silver nanoparticles
Biofilm
SEM-EDX
Pietrzak, Katarzyna
Otlewska, Anna
Puchalski, Michal
Gutarowska, Beata
Guiamet, Patricia Sandra
Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles
topic_facet Química
Física
Archaeological textiles
Pseudomonas
Protection
Silver nanoparticles
Biofilm
SEM-EDX
description The aims of this work were to: (i) microscopically analyse the pre- and post-Columbian archaeological textiles using Scanning Electron Microscopy with Energy Dispersive X-Ray Analysis (SEM-EDX); (ii) microbiologically analyse the archaeological textiles (from the Southern Andean Area, La Plata Museum); (iii) determine the ability of Pseudomonas sp. isolates from archaeological textiles to biofilm formation by SEM; (iv) assess the anti-biofilm properties of AgNPs protecting cotton against Pseudomonas sp. Results showed the presence of bacteria with proteolytic and lipolytic activities on archaeological textiles, including Clostridium sp. and Pseudomonas aeruginosa. Two nucleotide sequences of 16S ribosomal RNA gene of P. aeruginosa strains were deposited in GeneBank NCBI database with accession numbers: KP842564 (strain 1) and KP842565 (strain 2). Those strains exhibited different morphological and growth characteristics: strain 1 with ability to form biofilms on archaeological textiles was rod-shaped, produced bluish-green pigment, and smaller than strain 2; and strain 2 was pleomorphic and produced brown pigment. The use of silver nanoparticles (90 ppm, φ 10–80 nm) allowed to protecting textiles against P. aeruginosa growth by 63%–97%, depending on the strain and exposition time.
format Articulo
Articulo
author Pietrzak, Katarzyna
Otlewska, Anna
Puchalski, Michal
Gutarowska, Beata
Guiamet, Patricia Sandra
author_facet Pietrzak, Katarzyna
Otlewska, Anna
Puchalski, Michal
Gutarowska, Beata
Guiamet, Patricia Sandra
author_sort Pietrzak, Katarzyna
title Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles
title_short Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles
title_full Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles
title_fullStr Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles
title_full_unstemmed Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles
title_sort antimicrobial properties of silver nanoparticles against biofilm formation by pseudomonas aeruginosa on archaeological textiles
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/98113
https://ri.conicet.gov.ar/11336/64092
https://www.udspub.com/ajj/public/index.php/aeb/article/view/145/118
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AT puchalskimichal antimicrobialpropertiesofsilvernanoparticlesagainstbiofilmformationbypseudomonasaeruginosaonarchaeologicaltextiles
AT gutarowskabeata antimicrobialpropertiesofsilvernanoparticlesagainstbiofilmformationbypseudomonasaeruginosaonarchaeologicaltextiles
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bdutipo_str Repositorios
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