Fate of Bacillus cereus within phagocytic cells

In this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were asses...

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Detalles Bibliográficos
Autores principales: Rolny, Ivanna Sabrina, Racedo, Silvia María, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Bacillus cereus
Endocytic pathway
Intracellular trafficking
LAMP1
Phagocytosis
Virulence
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98111
https://ri.conicet.gov.ar/11336/57838
http://revistes.iec.cat/index.php/IM/article/view/144200
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98111
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bacillus cereus
Endocytic pathway
Intracellular trafficking
LAMP1
Phagocytosis
Virulence
spellingShingle Biología
Bacillus cereus
Endocytic pathway
Intracellular trafficking
LAMP1
Phagocytosis
Virulence
Rolny, Ivanna Sabrina
Racedo, Silvia María
Pérez, Pablo Fernando
Fate of Bacillus cereus within phagocytic cells
topic_facet Biología
Bacillus cereus
Endocytic pathway
Intracellular trafficking
LAMP1
Phagocytosis
Virulence
description In this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains of B. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquisition of lysosomal markers by B. cereus containing vacuoles (BcCV), assessed by LAMP1 and Lysotracker labelling occurred shortly after internalization. The highest ratio of LAMP1(+)-BcCV was found for strain M2. This strain was able to survive longer than strain B10502 which routes to LAMP1 containing vacuoles to a lesser extent. In addition, strain M2 stimulated expression of MHCII by infected cells. Confocal analyses 60 or 90 min post-infection showed different percentages of co-localization of bacteria with Lysotracker. Results suggest strain-dependent interaction and intracellular killing of B. cereus by phagocytic cells. These findings could be relevant for the pathogenic potential of Bacillus cereus strains.
format Articulo
Articulo
author Rolny, Ivanna Sabrina
Racedo, Silvia María
Pérez, Pablo Fernando
author_facet Rolny, Ivanna Sabrina
Racedo, Silvia María
Pérez, Pablo Fernando
author_sort Rolny, Ivanna Sabrina
title Fate of Bacillus cereus within phagocytic cells
title_short Fate of Bacillus cereus within phagocytic cells
title_full Fate of Bacillus cereus within phagocytic cells
title_fullStr Fate of Bacillus cereus within phagocytic cells
title_full_unstemmed Fate of Bacillus cereus within phagocytic cells
title_sort fate of bacillus cereus within phagocytic cells
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/98111
https://ri.conicet.gov.ar/11336/57838
http://revistes.iec.cat/index.php/IM/article/view/144200
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bdutipo_str Repositorios
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