Genotoxicity of cooper suplementation in cattle to prevent hypocuprosis

Copper is an essential micronutrient in cattle. Usually, the most common disease associated with copper is hypocuprosis. To prevent the copper deficiency, copper supplementation is indicated. Preliminary studies reported gentoxic effect of copper defficiency. Simultaneously, a gentoxic effect of cop...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Picco, Sebastián Julio, Mattioli, Guillermo Alberto, Dulout, Fernando Noel, Romero, Jorge Roberto, Rosa, Diana Esther, Fazzio, Luis Emilio, De Luca, Julio César
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Veterinarias
Genotoxicidad
Suplementación con cobre
Hipocuprosis bovina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98074
https://ri.conicet.gov.ar/11336/84847
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98074
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Genotoxicidad
Suplementación con cobre
Hipocuprosis bovina
spellingShingle Ciencias Veterinarias
Genotoxicidad
Suplementación con cobre
Hipocuprosis bovina
Picco, Sebastián Julio
Mattioli, Guillermo Alberto
Dulout, Fernando Noel
Romero, Jorge Roberto
Rosa, Diana Esther
Fazzio, Luis Emilio
De Luca, Julio César
Genotoxicity of cooper suplementation in cattle to prevent hypocuprosis
topic_facet Ciencias Veterinarias
Genotoxicidad
Suplementación con cobre
Hipocuprosis bovina
description Copper is an essential micronutrient in cattle. Usually, the most common disease associated with copper is hypocuprosis. To prevent the copper deficiency, copper supplementation is indicated. Preliminary studies reported gentoxic effect of copper defficiency. Simultaneously, a gentoxic effect of copper supplementation was detected. The aim of this work was to report the DNA damage, assessed by comet assay, associated with copper supplementation in Aberdeen Angus cattle. Blood samples were obtained from 22 normocupremic Aberdeen Angus cows (Group A) and from 20 Aberdeen Angus cows previously supplemented with parentheral copper (Group B). Copper plasma concentration was determined by flame atomic absorption spectrophotometry and DNA damage was assessed by alkaline comet assay. Copper plasma leves were 66.3 ±5.9 µg/dl and 72.2 ±8.6 µg/dl in group A and group B respectively. Comet assay showed a significant increase of nuclei with DNA migration in group B (46% ±  8.6) when it was compared with group A (29% ± 8.4) (p< 0.01). The degree of DNA damage showed 70.6 ± 8.39 degree 1 cells in group A and 50.4 ± 8.58 degree 1 cells in group B (p< 0.01). For degree 2 cells, the percentages were 19.5 ± 7.45 and 4.10 ± 9.5 for groups A and B respectively (p< 0.01). Results obtained showed a significant increase of DNA damage in normocupremic animals with copper supplementation, probably associated with the prooxidant effect of copper. Further studies could contribute to elucidate the mechanisms involved in the induction of DNA damage, as well as the effect of copper supplementation in farm animals.
format Articulo
Articulo
author Picco, Sebastián Julio
Mattioli, Guillermo Alberto
Dulout, Fernando Noel
Romero, Jorge Roberto
Rosa, Diana Esther
Fazzio, Luis Emilio
De Luca, Julio César
author_facet Picco, Sebastián Julio
Mattioli, Guillermo Alberto
Dulout, Fernando Noel
Romero, Jorge Roberto
Rosa, Diana Esther
Fazzio, Luis Emilio
De Luca, Julio César
author_sort Picco, Sebastián Julio
title Genotoxicity of cooper suplementation in cattle to prevent hypocuprosis
title_short Genotoxicity of cooper suplementation in cattle to prevent hypocuprosis
title_full Genotoxicity of cooper suplementation in cattle to prevent hypocuprosis
title_fullStr Genotoxicity of cooper suplementation in cattle to prevent hypocuprosis
title_full_unstemmed Genotoxicity of cooper suplementation in cattle to prevent hypocuprosis
title_sort genotoxicity of cooper suplementation in cattle to prevent hypocuprosis
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/98074
https://ri.conicet.gov.ar/11336/84847
work_keys_str_mv AT piccosebastianjulio genotoxicityofcoopersuplementationincattletopreventhypocuprosis
AT mattioliguillermoalberto genotoxicityofcoopersuplementationincattletopreventhypocuprosis
AT duloutfernandonoel genotoxicityofcoopersuplementationincattletopreventhypocuprosis
AT romerojorgeroberto genotoxicityofcoopersuplementationincattletopreventhypocuprosis
AT rosadianaesther genotoxicityofcoopersuplementationincattletopreventhypocuprosis
AT fazzioluisemilio genotoxicityofcoopersuplementationincattletopreventhypocuprosis
AT delucajuliocesar genotoxicityofcoopersuplementationincattletopreventhypocuprosis
bdutipo_str Repositorios
_version_ 1764820492602048516

Ejemplares similares