Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)

Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment ph...

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Detalles Bibliográficos
Autores principales: Medina, Rocío, Lucentini, César Gustavo, Franco, Mario Emilio Ernesto, Petroselli, Gabriela, Rosso, Janina Alejandra, Erra Balsells, Rosa, Balatti, Pedro Alberto, Saparrat, Mario Carlos Nazareno
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Biochemistry
Genetics
Microbiology
Molecular biology
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97948
https://ri.conicet.gov.ar/11336/88783
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97948
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Biochemistry
Genetics
Microbiology
Molecular biology
spellingShingle Biología
Biochemistry
Genetics
Microbiology
Molecular biology
Medina, Rocío
Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
topic_facet Biología
Biochemistry
Genetics
Microbiology
Molecular biology
description Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.
format Articulo
Articulo
author Medina, Rocío
Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
author_facet Medina, Rocío
Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
author_sort Medina, Rocío
title Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_short Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_full Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_fullStr Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_full_unstemmed Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_sort identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of fulvia fulva (syn. cladosporium fulvum)
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/97948
https://ri.conicet.gov.ar/11336/88783
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