Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels

After depletion of intracellular Ca<sup>2+</sup> stores the capacitative response triggers an extracellular Ca<sup>2+</sup> influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented...

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Autores principales: Roldán Palomo, Ana Rocío, Martín, Pedro, Rebolledo, Alejandro, Enrique, Nicolás Jorge, Flores, Luis Emilio, Milesi, María Verónica
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Exactas
Medicina
Human umbilical artery
Capacitative response
Trpc channels
Serotonin
Histamine
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97874
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97874
record_format dspace
spelling I19-R120-10915-978742024-06-14T18:51:51Z http://sedici.unlp.edu.ar/handle/10915/97874 Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels Roldán Palomo, Ana Rocío Martín, Pedro Rebolledo, Alejandro Enrique, Nicolás Jorge Flores, Luis Emilio Milesi, María Verónica 2012-08 2020-06-09T16:54:40Z en Ciencias Exactas Medicina Human umbilical artery Capacitative response Trpc channels Serotonin Histamine After depletion of intracellular Ca<sup>2+</sup> stores the capacitative response triggers an extracellular Ca<sup>2+</sup> influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca<sup>2+</sup> depletion by a Ca<sup>2+</sup>-free extracellular solution followed by Ca<sup>2+</sup> readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine was significantly less than a first one, likely because 2-APB inhibited store refilling by capacitative Ca<sup>2+</sup> entry. 2-APB inhibition of sarcoplasmic reticulum Ca<sup>2+</sup> release was excluded because this blocker did not affect serotonin force development in a Ca<sup>2+</sup>-free solution. The PCR technique showed the presence of mRNAs for STIM proteins (1 and 2), for Orai proteins (1, 2 and 3) and for TRPC channels (subtypes 1, 3, 4 and 6) in the smooth muscle of the human umbilical artery. Hence, this artery presents a capacitative contractile response triggered by stimulation with physiological vasoconstrictors and expresses mRNAs for proteins and channels previously identified as SOCs. Facultad de Ciencias Exactas Facultad de Ciencias Médicas Centro de Endocrinología Experimental y Aplicada Grupo de Investigación en Fisiología Vascular Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 73-81
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Medicina
Human umbilical artery
Capacitative response
Trpc channels
Serotonin
Histamine
spellingShingle Ciencias Exactas
Medicina
Human umbilical artery
Capacitative response
Trpc channels
Serotonin
Histamine
Roldán Palomo, Ana Rocío
Martín, Pedro
Rebolledo, Alejandro
Enrique, Nicolás Jorge
Flores, Luis Emilio
Milesi, María Verónica
Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels
topic_facet Ciencias Exactas
Medicina
Human umbilical artery
Capacitative response
Trpc channels
Serotonin
Histamine
description After depletion of intracellular Ca<sup>2+</sup> stores the capacitative response triggers an extracellular Ca<sup>2+</sup> influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca<sup>2+</sup> depletion by a Ca<sup>2+</sup>-free extracellular solution followed by Ca<sup>2+</sup> readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine was significantly less than a first one, likely because 2-APB inhibited store refilling by capacitative Ca<sup>2+</sup> entry. 2-APB inhibition of sarcoplasmic reticulum Ca<sup>2+</sup> release was excluded because this blocker did not affect serotonin force development in a Ca<sup>2+</sup>-free solution. The PCR technique showed the presence of mRNAs for STIM proteins (1 and 2), for Orai proteins (1, 2 and 3) and for TRPC channels (subtypes 1, 3, 4 and 6) in the smooth muscle of the human umbilical artery. Hence, this artery presents a capacitative contractile response triggered by stimulation with physiological vasoconstrictors and expresses mRNAs for proteins and channels previously identified as SOCs.
format Articulo
Articulo
author Roldán Palomo, Ana Rocío
Martín, Pedro
Rebolledo, Alejandro
Enrique, Nicolás Jorge
Flores, Luis Emilio
Milesi, María Verónica
author_facet Roldán Palomo, Ana Rocío
Martín, Pedro
Rebolledo, Alejandro
Enrique, Nicolás Jorge
Flores, Luis Emilio
Milesi, María Verónica
author_sort Roldán Palomo, Ana Rocío
title Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels
title_short Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels
title_full Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels
title_fullStr Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels
title_full_unstemmed Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels
title_sort human umbilical artery smooth muscle exhibits a 2-apb-sensitive capacitative contractile response evoked by vasoactive substances and expresses mrnas for stim, orai and trpc channels
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/97874
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