Combination of cloxacillin and essential oil of <i>Melaleuca armillaris</i> as an alternative against <i>Staphylococcus aureus</i>

The emergence of resistance to antibiotics has been favored by abuse in the application of antimicrobials in human and animal medicine. Essential oils are a great resource to deal with this crisis. <i>Melaleuca armillaris</i> belongs to the family of Myrtaceae, rich in species with essen...

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Autores principales: Buldain, Daniel Cornelio, Buchamer, Andrea Verónica, Marchetti, María Laura, Aliverti, Florencia, Bandoni, Arnaldo Luis, Mestorino, Olga Nora
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
Antimicrobial
Cloxacillin
Essential oil
Melaleuca armillaris
MRSA
Resistance
Staphyloccocus aureus
Synergism
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97763
https://ri.conicet.gov.ar/11336/83944
https://www.frontiersin.org/article/10.3389/fvets.2018.00177/full
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97763
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Antimicrobial
Cloxacillin
Essential oil
Melaleuca armillaris
MRSA
Resistance
Staphyloccocus aureus
Synergism
spellingShingle Ciencias Veterinarias
Antimicrobial
Cloxacillin
Essential oil
Melaleuca armillaris
MRSA
Resistance
Staphyloccocus aureus
Synergism
Buldain, Daniel Cornelio
Buchamer, Andrea Verónica
Marchetti, María Laura
Aliverti, Florencia
Bandoni, Arnaldo Luis
Mestorino, Olga Nora
Combination of cloxacillin and essential oil of <i>Melaleuca armillaris</i> as an alternative against <i>Staphylococcus aureus</i>
topic_facet Ciencias Veterinarias
Antimicrobial
Cloxacillin
Essential oil
Melaleuca armillaris
MRSA
Resistance
Staphyloccocus aureus
Synergism
description The emergence of resistance to antibiotics has been favored by abuse in the application of antimicrobials in human and animal medicine. Essential oils are a great resource to deal with this crisis. <i>Melaleuca armillaris</i> belongs to the family of Myrtaceae, rich in species with essential oils. Plant extracts has shown antimicrobial activity in many investigations. Cloxacillin (CLOX) is an antibiotic widely used in veterinary medicine against <i>Staphylococcus aureus</i>. Our aim was to assess pharmacodynamic interaction established by combining essential oil of <i>M. armillaris</i> (EO) with CLOX in search of a synergistic effect that maximizes the antibacterial activity against <i>S. aureus</i>. The EO was obtained by steam distillation and its composition was analyzed by a GC-FID-MS. The most abundant components in the EO were 1.8 cineole (72.3%), limonene (7.8%). and a-pinene (6%). We worked with wild type <i>S. aureus</i> strains (n = 3) isolated from Holstein cows, and <i>S. aureus</i> ATCC 29213 as the reference strain. The Minimum Inhibitory Concentration (MIC) of CLOX, EO and the combination was determined by microdilution in broth at pH 7.4; 6.5 and 5.0. The checkerboard method was applied to evaluate the interaction between CLOX and EO. The Fractional Inhibitory Concentration index (FIC) was established. From those combinations that yielded the lowest FIC values, we evaluated the index of antibacterial activity (E), established as the difference between the Log<sub>10</sub> values of the number of viable bacteria at the initial (nt<sub>0</sub>) and at the end of the test (nt<sub>24</sub>). So, time-killing curves with CLOX and EO/CLOX combination at 0.5, 1, 2, 4, and 8 fold the MIC in broth at pH 7.4; 6.5 and 5.0 were prepared. We considered Bacteriostatic effect (E = 0) Bactericidal effect (E = -3) and Effect of virtual eradication of bacteria (E = -4). A clear synergic activity between the EO and the CLOX was demonstrated, which allows reducing the MIC of β-lactam against <i>S. aureus</i>. This interaction was favored by acidification of the medium, where lower concentrations of CLOX achieved a bactericidal effect, close to virtual eradication, in the presence of small amounts of EO.
format Articulo
Articulo
author Buldain, Daniel Cornelio
Buchamer, Andrea Verónica
Marchetti, María Laura
Aliverti, Florencia
Bandoni, Arnaldo Luis
Mestorino, Olga Nora
author_facet Buldain, Daniel Cornelio
Buchamer, Andrea Verónica
Marchetti, María Laura
Aliverti, Florencia
Bandoni, Arnaldo Luis
Mestorino, Olga Nora
author_sort Buldain, Daniel Cornelio
title Combination of cloxacillin and essential oil of <i>Melaleuca armillaris</i> as an alternative against <i>Staphylococcus aureus</i>
title_short Combination of cloxacillin and essential oil of <i>Melaleuca armillaris</i> as an alternative against <i>Staphylococcus aureus</i>
title_full Combination of cloxacillin and essential oil of <i>Melaleuca armillaris</i> as an alternative against <i>Staphylococcus aureus</i>
title_fullStr Combination of cloxacillin and essential oil of <i>Melaleuca armillaris</i> as an alternative against <i>Staphylococcus aureus</i>
title_full_unstemmed Combination of cloxacillin and essential oil of <i>Melaleuca armillaris</i> as an alternative against <i>Staphylococcus aureus</i>
title_sort combination of cloxacillin and essential oil of <i>melaleuca armillaris</i> as an alternative against <i>staphylococcus aureus</i>
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/97763
https://ri.conicet.gov.ar/11336/83944
https://www.frontiersin.org/article/10.3389/fvets.2018.00177/full
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